Abstract
To define the significance of glutamate ionotropic receptors in sPLA -mediated neuronal cell death we used the NMDA receptor antagonist MK-801 and the AMPA receptor antagonist PNQX. In primary neuronal cell cultures both MK-801 and PNQX inhibited sPLA - and glutamate-induced neuronal death. [ H]Arachidonic acid release induced by both sPLA and glutamate was partially blocked by MK-801, indicating that the glutamate-NMDA-cPLA pathway contributes to sPLA -induced arachidonic acid release. Systemic administration of MK-801 to rats that had sPLA injected into the right striatum significantly decreased neuronal cell death. We conclude that glutamatergic synaptic activity modulates sPLA -induced neuronal cell death.
| Original language | English |
|---|---|
| Journal | NeuroReport |
| Volume | 13 |
| Issue number | 15 |
| Pages (from-to) | 1963-6 |
| Number of pages | 4 |
| ISSN | 0959-4965 |
| Publication status | Published - 28 Oct 2002 |
Keywords
- Animals
- Arachidonic Acid
- Astrocytes
- Body Temperature
- Brain
- Cell Death
- Cells, Cultured
- Cerebral Infarction
- Dizocilpine Maleate
- Dose-Response Relationship, Drug
- Excitatory Amino Acid Antagonists
- Excitatory Amino Acids
- Fetus
- Group II Phospholipases A2
- Humans
- Neostriatum
- Neurons
- Phospholipases A
- Phospholipases A2
- Quinoxalines
- Rats
- Rats, Wistar
- Receptors, AMPA
- Receptors, N-Methyl-D-Aspartate