Secretory IgA-mediated immune response in saliva and early detection of Pseudomonas aeruginosa in the lower airways of pediatric cystic fibrosis patients

Renan Marrichi Mauch; Claudio Lucio Rossi; Marcos Tadeu Nolasco da Silva; Talita Bianchi Aiello; José Dirceu Ribeiro; Antônio Fernando Ribeiro; Niels Høiby; Carlos Emilio Levy

doi:10.1007/s00430-019-00578-w

Secretory IgA-mediated immune response in saliva and early detection of Pseudomonas aeruginosa in the lower airways of pediatric cystic fibrosis patients

Renan Marrichi Mauch, Claudio Lucio Rossi, Marcos Tadeu Nolasco da Silva, Talita Bianchi Aiello, José Dirceu Ribeiro, Antônio Fernando Ribeiro, Niels Høiby, Carlos Emilio Levy^*

^*Corresponding author for this work

3 Citations (Scopus)

Abstract

Pseudomonas aeruginosa (Pa) detection in the paranasal sinuses may help to prevent or postpone bacterial aspiration to the lower airways (LAW) and chronic lung infection in cystic fibrosis (CF). We assessed the ability of an ELISA test for measurement of specific Pa secretory IgA (sIgA) in saliva (a potential marker of sinus colonization) to early detect changes in the Pa LAW status (indicated by microbiological sputum or cough swab culture and specific serum IgG levels) of 65 patients for three years, in different investigation scenarios. Increased sIgA levels were detected in saliva up to 22 months before changes in culture/serology. Patients who remained Pa-positive had significantly increased sIgA levels than patients who remained Pa-negative, both at the baseline (39.6 U/mL vs. 19.2 U/mL; p = 0.02) and at the end of the follow-up (119.4 U/mL vs. 25.2 U/mL; p < 0.001). No association was found between sIgA levels in saliva and emergence or recurrence of Pa in the LAW. A positive median sIgA result in the first year of follow-up implied up to 12.5-fold increased risk of subsequent Pa exposure in the LAW. Our test detected early changes in the P. aeruginosa LAW status and risk of exposure to P. aeruginosa in the LAW with two years in advance. Comparison with sinus culture is needed to assess the test’s ability to identify CF patients in need of a sinus approach for Pa investigation, which could provide opportunities of Pa eradication before its aspiration to the lungs.

Original language	English
Journal	Medical Microbiology and Immunology
Volume	208
Issue number	2
Pages (from-to)	205-213
Number of pages	9
ISSN	0300-8584
DOIs	https://doi.org/10.1007/s00430-019-00578-w
Publication status	Published - 2019

Keywords

Cystic fibrosis
Diagnosis
Pseudomonas aeruginosa
Respiratory tract infections
Saliva
Secretory IgA

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10.1007/s00430-019-00578-w

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Mauch, R. M., Rossi, C. L., Nolasco da Silva, M. T., Bianchi Aiello, T., Ribeiro, J. D., Ribeiro, A. F., Høiby, N., & Levy, C. E. (2019). Secretory IgA-mediated immune response in saliva and early detection of Pseudomonas aeruginosa in the lower airways of pediatric cystic fibrosis patients. Medical Microbiology and Immunology, 208(2), 205-213. https://doi.org/10.1007/s00430-019-00578-w

Secretory IgA-mediated immune response in saliva and early detection of Pseudomonas aeruginosa in the lower airways of pediatric cystic fibrosis patients. / Mauch, Renan Marrichi; Rossi, Claudio Lucio; Nolasco da Silva, Marcos Tadeu et al.
In: Medical Microbiology and Immunology, Vol. 208, No. 2, 2019, p. 205-213.

Research output: Contribution to journal › Journal article › Research › peer-review

Mauch, RM, Rossi, CL, Nolasco da Silva, MT, Bianchi Aiello, T, Ribeiro, JD, Ribeiro, AF, Høiby, N & Levy, CE 2019, 'Secretory IgA-mediated immune response in saliva and early detection of Pseudomonas aeruginosa in the lower airways of pediatric cystic fibrosis patients', Medical Microbiology and Immunology, vol. 208, no. 2, pp. 205-213. https://doi.org/10.1007/s00430-019-00578-w

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abstract = "Pseudomonas aeruginosa (Pa) detection in the paranasal sinuses may help to prevent or postpone bacterial aspiration to the lower airways (LAW) and chronic lung infection in cystic fibrosis (CF). We assessed the ability of an ELISA test for measurement of specific Pa secretory IgA (sIgA) in saliva (a potential marker of sinus colonization) to early detect changes in the Pa LAW status (indicated by microbiological sputum or cough swab culture and specific serum IgG levels) of 65 patients for three years, in different investigation scenarios. Increased sIgA levels were detected in saliva up to 22 months before changes in culture/serology. Patients who remained Pa-positive had significantly increased sIgA levels than patients who remained Pa-negative, both at the baseline (39.6 U/mL vs. 19.2 U/mL; p = 0.02) and at the end of the follow-up (119.4 U/mL vs. 25.2 U/mL; p < 0.001). No association was found between sIgA levels in saliva and emergence or recurrence of Pa in the LAW. A positive median sIgA result in the first year of follow-up implied up to 12.5-fold increased risk of subsequent Pa exposure in the LAW. Our test detected early changes in the P. aeruginosa LAW status and risk of exposure to P. aeruginosa in the LAW with two years in advance. Comparison with sinus culture is needed to assess the test{\textquoteright}s ability to identify CF patients in need of a sinus approach for Pa investigation, which could provide opportunities of Pa eradication before its aspiration to the lungs.",

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author = "Mauch, {Renan Marrichi} and Rossi, {Claudio Lucio} and {Nolasco da Silva}, {Marcos Tadeu} and {Bianchi Aiello}, Talita and Ribeiro, {Jos{\'e} Dirceu} and Ribeiro, {Ant{\^o}nio Fernando} and Niels H{\o}iby and Levy, {Carlos Emilio}",

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doi = "10.1007/s00430-019-00578-w",

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T1 - Secretory IgA-mediated immune response in saliva and early detection of Pseudomonas aeruginosa in the lower airways of pediatric cystic fibrosis patients

AU - Mauch, Renan Marrichi

AU - Rossi, Claudio Lucio

AU - Nolasco da Silva, Marcos Tadeu

AU - Bianchi Aiello, Talita

AU - Ribeiro, José Dirceu

AU - Ribeiro, Antônio Fernando

AU - Høiby, Niels

AU - Levy, Carlos Emilio

PY - 2019

Y1 - 2019

N2 - Pseudomonas aeruginosa (Pa) detection in the paranasal sinuses may help to prevent or postpone bacterial aspiration to the lower airways (LAW) and chronic lung infection in cystic fibrosis (CF). We assessed the ability of an ELISA test for measurement of specific Pa secretory IgA (sIgA) in saliva (a potential marker of sinus colonization) to early detect changes in the Pa LAW status (indicated by microbiological sputum or cough swab culture and specific serum IgG levels) of 65 patients for three years, in different investigation scenarios. Increased sIgA levels were detected in saliva up to 22 months before changes in culture/serology. Patients who remained Pa-positive had significantly increased sIgA levels than patients who remained Pa-negative, both at the baseline (39.6 U/mL vs. 19.2 U/mL; p = 0.02) and at the end of the follow-up (119.4 U/mL vs. 25.2 U/mL; p < 0.001). No association was found between sIgA levels in saliva and emergence or recurrence of Pa in the LAW. A positive median sIgA result in the first year of follow-up implied up to 12.5-fold increased risk of subsequent Pa exposure in the LAW. Our test detected early changes in the P. aeruginosa LAW status and risk of exposure to P. aeruginosa in the LAW with two years in advance. Comparison with sinus culture is needed to assess the test’s ability to identify CF patients in need of a sinus approach for Pa investigation, which could provide opportunities of Pa eradication before its aspiration to the lungs.

AB - Pseudomonas aeruginosa (Pa) detection in the paranasal sinuses may help to prevent or postpone bacterial aspiration to the lower airways (LAW) and chronic lung infection in cystic fibrosis (CF). We assessed the ability of an ELISA test for measurement of specific Pa secretory IgA (sIgA) in saliva (a potential marker of sinus colonization) to early detect changes in the Pa LAW status (indicated by microbiological sputum or cough swab culture and specific serum IgG levels) of 65 patients for three years, in different investigation scenarios. Increased sIgA levels were detected in saliva up to 22 months before changes in culture/serology. Patients who remained Pa-positive had significantly increased sIgA levels than patients who remained Pa-negative, both at the baseline (39.6 U/mL vs. 19.2 U/mL; p = 0.02) and at the end of the follow-up (119.4 U/mL vs. 25.2 U/mL; p < 0.001). No association was found between sIgA levels in saliva and emergence or recurrence of Pa in the LAW. A positive median sIgA result in the first year of follow-up implied up to 12.5-fold increased risk of subsequent Pa exposure in the LAW. Our test detected early changes in the P. aeruginosa LAW status and risk of exposure to P. aeruginosa in the LAW with two years in advance. Comparison with sinus culture is needed to assess the test’s ability to identify CF patients in need of a sinus approach for Pa investigation, which could provide opportunities of Pa eradication before its aspiration to the lungs.

KW - Cystic fibrosis

KW - Diagnosis

KW - Pseudomonas aeruginosa

KW - Respiratory tract infections

KW - Saliva

KW - Secretory IgA

U2 - 10.1007/s00430-019-00578-w

DO - 10.1007/s00430-019-00578-w

M3 - Journal article

C2 - 30706137

AN - SCOPUS:85060919596

SN - 0300-8584

VL - 208

SP - 205

EP - 213

JO - Medical Microbiology and Immunology

JF - Medical Microbiology and Immunology

IS - 2

ER -

Secretory IgA-mediated immune response in saliva and early detection of Pseudomonas aeruginosa in the lower airways of pediatric cystic fibrosis patients

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Keywords

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