Seasonal changes in nitrogen availability, and root and microbial uptake of 15N13C9-phenylalanine and 15N-ammonium in situ at a temperate heath

Louise C. Andresen; Anders Michelsen; Sven Evert Jonasson; Lena Ström

doi:10.1016/j.apsoil.2011.09.005

Seasonal changes in nitrogen availability, and root and microbial uptake of ¹⁵N¹³C₉-phenylalanine and ¹⁵N-ammonium in situ at a temperate heath

Louise C. Andresen, Anders Michelsen, Sven Evert Jonasson, Lena Ström

Terrestrial Ecology

12 Citations (Scopus)

Abstract

In the plant biosynthesis of secondary compounds, phenylalanine is a precursor of condensed tannins.
Tannins are deposited into the soil in plant root exudates and dead plant material and have been suggested to precipitate some soil nutrients and hence reduce nutrient availability for plants. Free amino acid,inorganic and microbial N concentration during the growing season was investigated in an ecosystem
with a natural tannin chemosphere. The influence of tannins on the uptake of nitrogen in plants and microbes was followed by injecting tannic acid (TA), ammonium-15N and phenylalanine-15N/13C9.
Plants preferred ammonium over phenylalanine, while microbes had no preference. Soil microbes had a 77% uptake of intact phenylalanine. Phenylalanine was acquired intact by both grasses and Calluna, with
63% and 38% uptake of intact phenylalanine in grass fine roots and Calluna roots, respectively. Inorganic N and amino acid concentrations were lowest in the period with highest plant activity and grass root biomass but were unaffected by TA addition.

Original language	English
Journal	Applied Soil Ecology
Volume	51
Pages (from-to)	94-101
Number of pages	8
ISSN	0929-1393
DOIs	https://doi.org/10.1016/j.apsoil.2011.09.005
Publication status	Published - Nov 2011

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10.1016/j.apsoil.2011.09.005

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@article{2d3d11990e4045f68c3d323b371002c5,

title = "Seasonal changes in nitrogen availability, and root and microbial uptake of 15N13C9-phenylalanine and 15N-ammonium in situ at a temperate heath",

abstract = "In the plant biosynthesis of secondary compounds, phenylalanine is a precursor of condensed tannins. Tannins are deposited into the soil in plant root exudates and dead plant material and have been suggested to precipitate some soil nutrients and hence reduce nutrient availability for plants. Free amino acid,inorganic and microbial N concentration during the growing season was investigated in an ecosystem with a natural tannin chemosphere. The influence of tannins on the uptake of nitrogen in plants and microbes was followed by injecting tannic acid (TA), ammonium-15N and phenylalanine-15N/13C9. Plants preferred ammonium over phenylalanine, while microbes had no preference. Soil microbes had a 77% uptake of intact phenylalanine. Phenylalanine was acquired intact by both grasses and Calluna, with 63% and 38% uptake of intact phenylalanine in grass fine roots and Calluna roots, respectively. Inorganic N and amino acid concentrations were lowest in the period with highest plant activity and grass root biomass but were unaffected by TA addition.",

author = "Andresen, {Louise C.} and Anders Michelsen and Jonasson, {Sven Evert} and Lena Str{\"o}m",

year = "2011",

month = nov,

doi = "10.1016/j.apsoil.2011.09.005",

language = "English",

volume = "51",

pages = "94--101",

journal = "Applied Soil Ecology",

issn = "0929-1393",

publisher = "Elsevier",

}

TY - JOUR

T1 - Seasonal changes in nitrogen availability, and root and microbial uptake of 15N13C9-phenylalanine and 15N-ammonium in situ at a temperate heath

AU - Andresen, Louise C.

AU - Michelsen, Anders

AU - Jonasson, Sven Evert

AU - Ström, Lena

PY - 2011/11

Y1 - 2011/11

N2 - In the plant biosynthesis of secondary compounds, phenylalanine is a precursor of condensed tannins. Tannins are deposited into the soil in plant root exudates and dead plant material and have been suggested to precipitate some soil nutrients and hence reduce nutrient availability for plants. Free amino acid,inorganic and microbial N concentration during the growing season was investigated in an ecosystem with a natural tannin chemosphere. The influence of tannins on the uptake of nitrogen in plants and microbes was followed by injecting tannic acid (TA), ammonium-15N and phenylalanine-15N/13C9. Plants preferred ammonium over phenylalanine, while microbes had no preference. Soil microbes had a 77% uptake of intact phenylalanine. Phenylalanine was acquired intact by both grasses and Calluna, with 63% and 38% uptake of intact phenylalanine in grass fine roots and Calluna roots, respectively. Inorganic N and amino acid concentrations were lowest in the period with highest plant activity and grass root biomass but were unaffected by TA addition.

AB - In the plant biosynthesis of secondary compounds, phenylalanine is a precursor of condensed tannins. Tannins are deposited into the soil in plant root exudates and dead plant material and have been suggested to precipitate some soil nutrients and hence reduce nutrient availability for plants. Free amino acid,inorganic and microbial N concentration during the growing season was investigated in an ecosystem with a natural tannin chemosphere. The influence of tannins on the uptake of nitrogen in plants and microbes was followed by injecting tannic acid (TA), ammonium-15N and phenylalanine-15N/13C9. Plants preferred ammonium over phenylalanine, while microbes had no preference. Soil microbes had a 77% uptake of intact phenylalanine. Phenylalanine was acquired intact by both grasses and Calluna, with 63% and 38% uptake of intact phenylalanine in grass fine roots and Calluna roots, respectively. Inorganic N and amino acid concentrations were lowest in the period with highest plant activity and grass root biomass but were unaffected by TA addition.

U2 - 10.1016/j.apsoil.2011.09.005

DO - 10.1016/j.apsoil.2011.09.005

M3 - Journal article

SN - 0929-1393

VL - 51

SP - 94

EP - 101

JO - Applied Soil Ecology

JF - Applied Soil Ecology

ER -