Wichmann, O., Muehlberger, N., Jelinek, T., Alifrangis, M., Peyerl-Hoffmann, G., Muhlen, M., Grobusch, M. P., Gascon, J., Matteelli, A., Laferl, H., Bisoffi, Z., Ehrhardt, S., Cuadros, J., Hatz, C., Gjorup, I., McWhinney, P., Beran, J., da Cunha, S., Schulze, M., ... European Network on Surveillance of Imported Infectious Diseases (2004). Screening for mutations related to atovaquone/proguanil resistance in treatment failures and other imported isolates of Plasmodium falciparum in Europe. Journal of Infectious Diseases, 190(9), 1541-6. https://doi.org/10.1086/424469
Screening for mutations related to atovaquone/proguanil resistance in treatment failures and other imported isolates of Plasmodium falciparum in Europe. / Wichmann, Ole; Muehlberger, Nikolai; Jelinek, Tomas et al.
In:
Journal of Infectious Diseases, Vol. 190, No. 9, 2004, p. 1541-6.
Research output: Contribution to journal › Journal article › Research › peer-review
Wichmann, O, Muehlberger, N, Jelinek, T, Alifrangis, M, Peyerl-Hoffmann, G, Muhlen, M, Grobusch, MP, Gascon, J, Matteelli, A, Laferl, H, Bisoffi, Z, Ehrhardt, S, Cuadros, J, Hatz, C, Gjorup, I, McWhinney, P, Beran, J, da Cunha, S, Schulze, M, Kollaritsch, H, Kern, P, Fry, G, Richter, J & European Network on Surveillance of Imported Infectious Diseases 2004, 'Screening for mutations related to atovaquone/proguanil resistance in treatment failures and other imported isolates of Plasmodium falciparum in Europe', Journal of Infectious Diseases, vol. 190, no. 9, pp. 1541-6. https://doi.org/10.1086/424469
Wichmann O, Muehlberger N, Jelinek T, Alifrangis M, Peyerl-Hoffmann G, Muhlen M et al. Screening for mutations related to atovaquone/proguanil resistance in treatment failures and other imported isolates of Plasmodium falciparum in Europe. Journal of Infectious Diseases. 2004;190(9):1541-6. doi: 10.1086/424469
Wichmann, Ole ; Muehlberger, Nikolai ; Jelinek, Tomas et al. / Screening for mutations related to atovaquone/proguanil resistance in treatment failures and other imported isolates of Plasmodium falciparum in Europe. In: Journal of Infectious Diseases. 2004 ; Vol. 190, No. 9. pp. 1541-6.
@article{aaac9370a99911ddb5e9000ea68e967b,
title = "Screening for mutations related to atovaquone/proguanil resistance in treatment failures and other imported isolates of Plasmodium falciparum in Europe",
abstract = "BACKGROUND: Two single-point mutations of the Plasmodium falciparum cytochrome b gene (Tyr268Asn and Tyr268Ser) were recently reported in cases of atovaquone/proguanil (Malarone) treatment failure. However, little is known about the prevalence of codon-268 mutations and their quantitative association with treatment failure. METHODS: We set out to assess the prevalence of codon-268 mutations in P. falciparum isolates imported into Europe and to quantify their association with atovaquone/proguanil treatment failure. Isolates of P. falciparum collected by the European Network on Imported Infectious Disease Surveillance between April 2000 and August 2003 were analyzed for codon-268 mutations, by use of polymerase chain reaction-restriction fragment-length polymorphism. RESULTS: We successfully screened 504 samples for the presence of either Tyr268Ser or Tyr268Asn. One case of Ser268 and no cases of Asn268 were detected. Therefore, we can be 95% confident that the prevalence of Ser268 in the European patient pool does not exceed 0.96% and that Asn268 is less frequent than 0.77%. In 58 patients treated with atovaquone/proguanil, Tyr268Ser was present in 1 of 5 patients with treatment failure but in 0 of 53 successfully treated patients. CONCLUSIONS: Tyr268Ser seems to be a sufficient, but not a necessary, cause for atovaquone/proguanil treatment failure. The prevalence of both codon-268 mutations is currently unlikely to be >1% in the European patient pool.",
author = "Ole Wichmann and Nikolai Muehlberger and Tomas Jelinek and Michael Alifrangis and Gabriele Peyerl-Hoffmann and Marion Muhlen and Grobusch, {Martin P} and Joaquim Gascon and Alberto Matteelli and Hermann Laferl and Zeno Bisoffi and Stephan Ehrhardt and Juan Cuadros and Christoph Hatz and Ida Gjorup and Paul McWhinney and Jiri Beran and {da Cunha}, Saraiva and Marco Schulze and Herwig Kollaritsch and Peter Kern and Graham Fry and Joachim Richter and {European Network on Surveillance of Imported Infectious Diseases}",
note = "Keywords: Adolescent; Adult; Aged; Amino Acid Substitution; Animals; Antimalarials; Atovaquone; Chloroguanide; Codon; Cytochromes b; DNA, Protozoan; Drug Combinations; Drug Resistance; Epidemiology, Molecular; Europe; Female; Genes, Protozoan; Humans; Malaria, Falciparum; Male; Middle Aged; Mutation, Missense; Naphthoquinones; Plasmodium falciparum; Point Mutation; Polymorphism, Restriction Fragment Length; Protozoan Proteins; Treatment Failure",
year = "2004",
doi = "10.1086/424469",
language = "English",
volume = "190",
pages = "1541--6",
journal = "Journal of Infectious Diseases",
issn = "0022-1899",
publisher = "Oxford University Press",
number = "9",
}
TY - JOUR
T1 - Screening for mutations related to atovaquone/proguanil resistance in treatment failures and other imported isolates of Plasmodium falciparum in Europe
AU - Wichmann, Ole
AU - Muehlberger, Nikolai
AU - Jelinek, Tomas
AU - Alifrangis, Michael
AU - Peyerl-Hoffmann, Gabriele
AU - Muhlen, Marion
AU - Grobusch, Martin P
AU - Gascon, Joaquim
AU - Matteelli, Alberto
AU - Laferl, Hermann
AU - Bisoffi, Zeno
AU - Ehrhardt, Stephan
AU - Cuadros, Juan
AU - Hatz, Christoph
AU - Gjorup, Ida
AU - McWhinney, Paul
AU - Beran, Jiri
AU - da Cunha, Saraiva
AU - Schulze, Marco
AU - Kollaritsch, Herwig
AU - Kern, Peter
AU - Fry, Graham
AU - Richter, Joachim
AU - European Network on Surveillance of Imported Infectious Diseases
N1 - Keywords: Adolescent; Adult; Aged; Amino Acid Substitution; Animals; Antimalarials; Atovaquone; Chloroguanide; Codon; Cytochromes b; DNA, Protozoan; Drug Combinations; Drug Resistance; Epidemiology, Molecular; Europe; Female; Genes, Protozoan; Humans; Malaria, Falciparum; Male; Middle Aged; Mutation, Missense; Naphthoquinones; Plasmodium falciparum; Point Mutation; Polymorphism, Restriction Fragment Length; Protozoan Proteins; Treatment Failure
PY - 2004
Y1 - 2004
N2 - BACKGROUND: Two single-point mutations of the Plasmodium falciparum cytochrome b gene (Tyr268Asn and Tyr268Ser) were recently reported in cases of atovaquone/proguanil (Malarone) treatment failure. However, little is known about the prevalence of codon-268 mutations and their quantitative association with treatment failure. METHODS: We set out to assess the prevalence of codon-268 mutations in P. falciparum isolates imported into Europe and to quantify their association with atovaquone/proguanil treatment failure. Isolates of P. falciparum collected by the European Network on Imported Infectious Disease Surveillance between April 2000 and August 2003 were analyzed for codon-268 mutations, by use of polymerase chain reaction-restriction fragment-length polymorphism. RESULTS: We successfully screened 504 samples for the presence of either Tyr268Ser or Tyr268Asn. One case of Ser268 and no cases of Asn268 were detected. Therefore, we can be 95% confident that the prevalence of Ser268 in the European patient pool does not exceed 0.96% and that Asn268 is less frequent than 0.77%. In 58 patients treated with atovaquone/proguanil, Tyr268Ser was present in 1 of 5 patients with treatment failure but in 0 of 53 successfully treated patients. CONCLUSIONS: Tyr268Ser seems to be a sufficient, but not a necessary, cause for atovaquone/proguanil treatment failure. The prevalence of both codon-268 mutations is currently unlikely to be >1% in the European patient pool.
AB - BACKGROUND: Two single-point mutations of the Plasmodium falciparum cytochrome b gene (Tyr268Asn and Tyr268Ser) were recently reported in cases of atovaquone/proguanil (Malarone) treatment failure. However, little is known about the prevalence of codon-268 mutations and their quantitative association with treatment failure. METHODS: We set out to assess the prevalence of codon-268 mutations in P. falciparum isolates imported into Europe and to quantify their association with atovaquone/proguanil treatment failure. Isolates of P. falciparum collected by the European Network on Imported Infectious Disease Surveillance between April 2000 and August 2003 were analyzed for codon-268 mutations, by use of polymerase chain reaction-restriction fragment-length polymorphism. RESULTS: We successfully screened 504 samples for the presence of either Tyr268Ser or Tyr268Asn. One case of Ser268 and no cases of Asn268 were detected. Therefore, we can be 95% confident that the prevalence of Ser268 in the European patient pool does not exceed 0.96% and that Asn268 is less frequent than 0.77%. In 58 patients treated with atovaquone/proguanil, Tyr268Ser was present in 1 of 5 patients with treatment failure but in 0 of 53 successfully treated patients. CONCLUSIONS: Tyr268Ser seems to be a sufficient, but not a necessary, cause for atovaquone/proguanil treatment failure. The prevalence of both codon-268 mutations is currently unlikely to be >1% in the European patient pool.
U2 - 10.1086/424469
DO - 10.1086/424469
M3 - Journal article
C2 - 15478057
SN - 0022-1899
VL - 190
SP - 1541
EP - 1546
JO - Journal of Infectious Diseases
JF - Journal of Infectious Diseases
IS - 9
ER -
