Salivary tissue inhibitor of metalloproteinases-1 localization and glycosylation profile analysis

Lars Holten-Andersen; Morten Thaysen-Andersen; Siri Beier Jensen; Christian von Buchwald; Peter Højrup; Hanne Kjær Offenberg; Hans Jørgen Nielsen; Nils Brünner; Birgitte Nauntofte; Jesper Reibel

doi:10.1111/j.1600-0463.2011.02796.x

Salivary tissue inhibitor of metalloproteinases-1 localization and glycosylation profile analysis

Lars Holten-Andersen, Morten Thaysen-Andersen, Siri Beier Jensen, Christian von Buchwald, Peter Højrup, Hanne Kjær Offenberg, Hans Jørgen Nielsen, Nils Brünner, Birgitte Nauntofte, Jesper Reibel

5 Citations (Scopus)

Abstract

Salivary tissue inhibitor of metalloproteinases-1 (TIMP-1) has been associated with pathological conditions in the oral cavity, but the origin of TIMP-1 in saliva remains unknown. Hence, we studied the localization of TIMP-1 in salivary gland tissue and also investigated if TIMP-1 found in blood and saliva is identical. Human salivary gland tissue samples (four parotid gland and four submandibular gland biopsies) were analysed for the presence of TIMP-1 mRNA and protein expression. To assess TIMP-1 glycosylation profiles in blood and saliva, the protein was isolated from plasma and unstimulated and stimulated whole saliva as well as stimulated parotid and submandibular saliva and analysed by MALDI-TOF mass spectrometry. TIMP-1 protein was demonstrated in mucous acinar cells of the submandibular gland and in ductal cells of both the parotid and submandibular gland. However, no TIMP-1 mRNA was detected in any of these cells. The glycosylation profiles of TIMP-1 isolated from whole saliva and saliva from the major glands were highly similar. In contrast, a significant difference was found between the glycoprofiles of salivary TIMP-1 and plasma TIMP-1. Although no clear evidence of TIMP-1 transcription in major salivary glands was demonstrated our results suggest that TIMP-1 in saliva does not originate from plasma.

Original language	English
Journal	A P M I S. Acta Pathologica, Microbiologica et Immunologica Scandinavica
Volume	119
Issue number	11
Pages (from-to)	741-749
Number of pages	9
ISSN	0903-4641
DOIs	https://doi.org/10.1111/j.1600-0463.2011.02796.x
Publication status	Published - Nov 2011

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Holten-Andersen, L., Thaysen-Andersen, M., Jensen, S. B., von Buchwald, C., Højrup, P., Offenberg, H. K., Nielsen, H. J., Brünner, N., Nauntofte, B., & Reibel, J. (2011). Salivary tissue inhibitor of metalloproteinases-1 localization and glycosylation profile analysis. A P M I S. Acta Pathologica, Microbiologica et Immunologica Scandinavica, 119(11), 741-749. https://doi.org/10.1111/j.1600-0463.2011.02796.x

Salivary tissue inhibitor of metalloproteinases-1 localization and glycosylation profile analysis. / Holten-Andersen, Lars; Thaysen-Andersen, Morten; Jensen, Siri Beier et al.

In: A P M I S. Acta Pathologica, Microbiologica et Immunologica Scandinavica, Vol. 119, No. 11, 11.2011, p. 741-749.

Research output: Contribution to journal › Journal article › Research › peer-review

Holten-Andersen, L, Thaysen-Andersen, M, Jensen, SB, von Buchwald, C, Højrup, P, Offenberg, HK, Nielsen, HJ, Brünner, N, Nauntofte, B & Reibel, J 2011, 'Salivary tissue inhibitor of metalloproteinases-1 localization and glycosylation profile analysis', A P M I S. Acta Pathologica, Microbiologica et Immunologica Scandinavica, vol. 119, no. 11, pp. 741-749. https://doi.org/10.1111/j.1600-0463.2011.02796.x

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abstract = "Salivary tissue inhibitor of metalloproteinases-1 (TIMP-1) has been associated with pathological conditions in the oral cavity, but the origin of TIMP-1 in saliva remains unknown. Hence, we studied the localization of TIMP-1 in salivary gland tissue and also investigated if TIMP-1 found in blood and saliva is identical. Human salivary gland tissue samples (four parotid gland and four submandibular gland biopsies) were analysed for the presence of TIMP-1 mRNA and protein expression. To assess TIMP-1 glycosylation profiles in blood and saliva, the protein was isolated from plasma and unstimulated and stimulated whole saliva as well as stimulated parotid and submandibular saliva and analysed by MALDI-TOF mass spectrometry. TIMP-1 protein was demonstrated in mucous acinar cells of the submandibular gland and in ductal cells of both the parotid and submandibular gland. However, no TIMP-1 mRNA was detected in any of these cells. The glycosylation profiles of TIMP-1 isolated from whole saliva and saliva from the major glands were highly similar. In contrast, a significant difference was found between the glycoprofiles of salivary TIMP-1 and plasma TIMP-1. Although no clear evidence of TIMP-1 transcription in major salivary glands was demonstrated our results suggest that TIMP-1 in saliva does not originate from plasma.",

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AB - Salivary tissue inhibitor of metalloproteinases-1 (TIMP-1) has been associated with pathological conditions in the oral cavity, but the origin of TIMP-1 in saliva remains unknown. Hence, we studied the localization of TIMP-1 in salivary gland tissue and also investigated if TIMP-1 found in blood and saliva is identical. Human salivary gland tissue samples (four parotid gland and four submandibular gland biopsies) were analysed for the presence of TIMP-1 mRNA and protein expression. To assess TIMP-1 glycosylation profiles in blood and saliva, the protein was isolated from plasma and unstimulated and stimulated whole saliva as well as stimulated parotid and submandibular saliva and analysed by MALDI-TOF mass spectrometry. TIMP-1 protein was demonstrated in mucous acinar cells of the submandibular gland and in ductal cells of both the parotid and submandibular gland. However, no TIMP-1 mRNA was detected in any of these cells. The glycosylation profiles of TIMP-1 isolated from whole saliva and saliva from the major glands were highly similar. In contrast, a significant difference was found between the glycoprofiles of salivary TIMP-1 and plasma TIMP-1. Although no clear evidence of TIMP-1 transcription in major salivary glands was demonstrated our results suggest that TIMP-1 in saliva does not originate from plasma.

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Salivary tissue inhibitor of metalloproteinases-1 localization and glycosylation profile analysis

Abstract

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