rs2735383, located at a microRNA binding site in the 3'UTR of NBS1, is not associated with breast cancer risk

Jingjing Liu; Ivona Lončar; J Margriet Collée; Manjeet K Bolla; Joe Dennis; Kyriaki Michailidou; Qin Wang; Irene L Andrulis; Monica Barile; Matthias W Beckmann; Sabine Behrens; Javier Benitez; Carl Blomqvist; Bram Boeckx; Natalia V Bogdanova; Stig E Bojesen; Hiltrud Brauch; Paul Brennan; Hermann Brenner; Annegien Broeks; Barbara Burwinkel; Jenny Chang-Claude; Shou-Tung Chen; Georgia Chenevix-Trench; Ching Y Cheng; Ji-Yeob Choi; Fergus J Couch; Angela Cox; Simon S Cross; Katarina Cuk; Kamila Czene; Thilo Dörk; Isabel Dos-Santos-Silva; Peter A Fasching; Jonine Figueroa; Henrik Flyger; Montserrat García-Closas; Graham G Giles; Gord Glendon; Mark S Goldberg; Anna González-Neira; Pascal Guénel; Christopher A Haiman; Ute Hamann; Steven N Hart; Mikael Hartman; Sigrid Hatse; John L Hopper; Hidemi Ito; Anna Jakubowska; NBCS Collaborators

doi:10.1038/srep36874

rs2735383, located at a microRNA binding site in the 3'UTR of NBS1, is not associated with breast cancer risk

Jingjing Liu, Ivona Lončar, J Margriet Collée, Manjeet K Bolla, Joe Dennis, Kyriaki Michailidou, Qin Wang, Irene L Andrulis, Monica Barile, Matthias W Beckmann, Sabine Behrens, Javier Benitez, Carl Blomqvist, Bram Boeckx, Natalia V Bogdanova, Stig E Bojesen, Hiltrud Brauch, Paul Brennan, Hermann Brenner, Annegien BroeksBarbara Burwinkel, Jenny Chang-Claude, Shou-Tung Chen, Georgia Chenevix-Trench, Ching Y Cheng, Ji-Yeob Choi, Fergus J Couch, Angela Cox, Simon S Cross, Katarina Cuk, Kamila Czene, Thilo Dörk, Isabel Dos-Santos-Silva, Peter A Fasching, Jonine Figueroa, Henrik Flyger, Montserrat García-Closas, Graham G Giles, Gord Glendon, Mark S Goldberg, Anna González-Neira, Pascal Guénel, Christopher A Haiman, Ute Hamann, Steven N Hart, Mikael Hartman, Sigrid Hatse, John L Hopper, Hidemi Ito, Anna Jakubowska, NBCS Collaborators

Department of Clinical Medicine

1 Citation (Scopus)

Abstract

NBS1, also known as NBN, plays an important role in maintaining genomic stability. Interestingly, rs2735383 G > C, located in a microRNA binding site in the 3'-untranslated region (UTR) of NBS1, was shown to be associated with increased susceptibility to lung and colorectal cancer. However, the relation between rs2735383 and susceptibility to breast cancer is not yet clear. Therefore, we genotyped rs2735383 in 1,170 familial non-BRCA1/2 breast cancer cases and 1,077 controls using PCR-based restriction fragment length polymorphism (RFLP-PCR) analysis, but found no association between rs2735383CC and breast cancer risk (OR = 1.214, 95% CI = 0.936-1.574, P = 0.144). Because we could not exclude a small effect size due to a limited sample size, we further analyzed imputed rs2735383 genotypes (r(2) > 0.999) of 47,640 breast cancer cases and 46,656 controls from the Breast Cancer Association Consortium (BCAC). However, rs2735383CC was not associated with overall breast cancer risk in European (OR = 1.014, 95% CI = 0.969-1.060, P = 0.556) nor in Asian women (OR = 0.998, 95% CI = 0.905-1.100, P = 0.961). Subgroup analyses by age, age at menarche, age at menopause, menopausal status, number of pregnancies, breast feeding, family history and receptor status also did not reveal a significant association. This study therefore does not support the involvement of the genotype at NBS1 rs2735383 in breast cancer susceptibility.

Original language	English
Article number	36874
Journal	Scientific Reports
Volume	6
Number of pages	13
ISSN	2045-2322
DOIs	https://doi.org/10.1038/srep36874
Publication status	Published - 15 Nov 2016

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Liu, J., Lončar, I., Collée, J. M., Bolla, M. K., Dennis, J., Michailidou, K., Wang, Q., Andrulis, I. L., Barile, M., Beckmann, M. W., Behrens, S., Benitez, J., Blomqvist, C., Boeckx, B., Bogdanova, N. V., Bojesen, S. E., Brauch, H., Brennan, P., Brenner, H., ... NBCS Collaborators (2016). rs2735383, located at a microRNA binding site in the 3'UTR of NBS1, is not associated with breast cancer risk. Scientific Reports, 6, Article 36874. https://doi.org/10.1038/srep36874

Liu, J, Lončar, I, Collée, JM, Bolla, MK, Dennis, J, Michailidou, K, Wang, Q, Andrulis, IL, Barile, M, Beckmann, MW, Behrens, S, Benitez, J, Blomqvist, C, Boeckx, B, Bogdanova, NV, Bojesen, SE, Brauch, H, Brennan, P, Brenner, H, Broeks, A, Burwinkel, B, Chang-Claude, J, Chen, S-T, Chenevix-Trench, G, Cheng, CY, Choi, J-Y, Couch, FJ, Cox, A, Cross, SS, Cuk, K, Czene, K, Dörk, T, Dos-Santos-Silva, I, Fasching, PA, Figueroa, J, Flyger, H, García-Closas, M, Giles, GG, Glendon, G, Goldberg, MS, González-Neira, A, Guénel, P, Haiman, CA, Hamann, U, Hart, SN, Hartman, M, Hatse, S, Hopper, JL, Ito, H, Jakubowska, A & NBCS Collaborators 2016, 'rs2735383, located at a microRNA binding site in the 3'UTR of NBS1, is not associated with breast cancer risk', Scientific Reports, vol. 6, 36874. https://doi.org/10.1038/srep36874

@article{68749cca0e27444f8aa1a86d68373bee,

title = "rs2735383, located at a microRNA binding site in the 3'UTR of NBS1, is not associated with breast cancer risk",

abstract = "NBS1, also known as NBN, plays an important role in maintaining genomic stability. Interestingly, rs2735383 G > C, located in a microRNA binding site in the 3'-untranslated region (UTR) of NBS1, was shown to be associated with increased susceptibility to lung and colorectal cancer. However, the relation between rs2735383 and susceptibility to breast cancer is not yet clear. Therefore, we genotyped rs2735383 in 1,170 familial non-BRCA1/2 breast cancer cases and 1,077 controls using PCR-based restriction fragment length polymorphism (RFLP-PCR) analysis, but found no association between rs2735383CC and breast cancer risk (OR = 1.214, 95% CI = 0.936-1.574, P = 0.144). Because we could not exclude a small effect size due to a limited sample size, we further analyzed imputed rs2735383 genotypes (r(2) > 0.999) of 47,640 breast cancer cases and 46,656 controls from the Breast Cancer Association Consortium (BCAC). However, rs2735383CC was not associated with overall breast cancer risk in European (OR = 1.014, 95% CI = 0.969-1.060, P = 0.556) nor in Asian women (OR = 0.998, 95% CI = 0.905-1.100, P = 0.961). Subgroup analyses by age, age at menarche, age at menopause, menopausal status, number of pregnancies, breast feeding, family history and receptor status also did not reveal a significant association. This study therefore does not support the involvement of the genotype at NBS1 rs2735383 in breast cancer susceptibility.",

author = "Jingjing Liu and Ivona Lon{\v c}ar and Coll{\'e}e, {J Margriet} and Bolla, {Manjeet K} and Joe Dennis and Kyriaki Michailidou and Qin Wang and Andrulis, {Irene L} and Monica Barile and Beckmann, {Matthias W} and Sabine Behrens and Javier Benitez and Carl Blomqvist and Bram Boeckx and Bogdanova, {Natalia V} and Bojesen, {Stig E} and Hiltrud Brauch and Paul Brennan and Hermann Brenner and Annegien Broeks and Barbara Burwinkel and Jenny Chang-Claude and Shou-Tung Chen and Georgia Chenevix-Trench and Cheng, {Ching Y} and Ji-Yeob Choi and Couch, {Fergus J} and Angela Cox and Cross, {Simon S} and Katarina Cuk and Kamila Czene and Thilo D{\"o}rk and Isabel Dos-Santos-Silva and Fasching, {Peter A} and Jonine Figueroa and Henrik Flyger and Montserrat Garc{\'i}a-Closas and Giles, {Graham G} and Gord Glendon and Goldberg, {Mark S} and Anna Gonz{\'a}lez-Neira and Pascal Gu{\'e}nel and Haiman, {Christopher A} and Ute Hamann and Hart, {Steven N} and Mikael Hartman and Sigrid Hatse and Hopper, {John L} and Hidemi Ito and Anna Jakubowska and {NBCS Collaborators}",

year = "2016",

month = nov,

day = "15",

doi = "10.1038/srep36874",

language = "English",

volume = "6",

journal = "Scientific Reports",

issn = "2045-2322",

publisher = "nature publishing group",

}

TY - JOUR

T1 - rs2735383, located at a microRNA binding site in the 3'UTR of NBS1, is not associated with breast cancer risk

AU - Liu, Jingjing

AU - Lončar, Ivona

AU - Collée, J Margriet

AU - Bolla, Manjeet K

AU - Dennis, Joe

AU - Michailidou, Kyriaki

AU - Wang, Qin

AU - Andrulis, Irene L

AU - Barile, Monica

AU - Beckmann, Matthias W

AU - Behrens, Sabine

AU - Benitez, Javier

AU - Blomqvist, Carl

AU - Boeckx, Bram

AU - Bogdanova, Natalia V

AU - Bojesen, Stig E

AU - Brauch, Hiltrud

AU - Brennan, Paul

AU - Brenner, Hermann

AU - Broeks, Annegien

AU - Burwinkel, Barbara

AU - Chang-Claude, Jenny

AU - Chen, Shou-Tung

AU - Chenevix-Trench, Georgia

AU - Cheng, Ching Y

AU - Choi, Ji-Yeob

AU - Couch, Fergus J

AU - Cox, Angela

AU - Cross, Simon S

AU - Cuk, Katarina

AU - Czene, Kamila

AU - Dörk, Thilo

AU - Dos-Santos-Silva, Isabel

AU - Fasching, Peter A

AU - Figueroa, Jonine

AU - Flyger, Henrik

AU - García-Closas, Montserrat

AU - Giles, Graham G

AU - Glendon, Gord

AU - Goldberg, Mark S

AU - González-Neira, Anna

AU - Guénel, Pascal

AU - Haiman, Christopher A

AU - Hamann, Ute

AU - Hart, Steven N

AU - Hartman, Mikael

AU - Hatse, Sigrid

AU - Hopper, John L

AU - Ito, Hidemi

AU - Jakubowska, Anna

AU - NBCS Collaborators

PY - 2016/11/15

Y1 - 2016/11/15

N2 - NBS1, also known as NBN, plays an important role in maintaining genomic stability. Interestingly, rs2735383 G > C, located in a microRNA binding site in the 3'-untranslated region (UTR) of NBS1, was shown to be associated with increased susceptibility to lung and colorectal cancer. However, the relation between rs2735383 and susceptibility to breast cancer is not yet clear. Therefore, we genotyped rs2735383 in 1,170 familial non-BRCA1/2 breast cancer cases and 1,077 controls using PCR-based restriction fragment length polymorphism (RFLP-PCR) analysis, but found no association between rs2735383CC and breast cancer risk (OR = 1.214, 95% CI = 0.936-1.574, P = 0.144). Because we could not exclude a small effect size due to a limited sample size, we further analyzed imputed rs2735383 genotypes (r(2) > 0.999) of 47,640 breast cancer cases and 46,656 controls from the Breast Cancer Association Consortium (BCAC). However, rs2735383CC was not associated with overall breast cancer risk in European (OR = 1.014, 95% CI = 0.969-1.060, P = 0.556) nor in Asian women (OR = 0.998, 95% CI = 0.905-1.100, P = 0.961). Subgroup analyses by age, age at menarche, age at menopause, menopausal status, number of pregnancies, breast feeding, family history and receptor status also did not reveal a significant association. This study therefore does not support the involvement of the genotype at NBS1 rs2735383 in breast cancer susceptibility.

AB - NBS1, also known as NBN, plays an important role in maintaining genomic stability. Interestingly, rs2735383 G > C, located in a microRNA binding site in the 3'-untranslated region (UTR) of NBS1, was shown to be associated with increased susceptibility to lung and colorectal cancer. However, the relation between rs2735383 and susceptibility to breast cancer is not yet clear. Therefore, we genotyped rs2735383 in 1,170 familial non-BRCA1/2 breast cancer cases and 1,077 controls using PCR-based restriction fragment length polymorphism (RFLP-PCR) analysis, but found no association between rs2735383CC and breast cancer risk (OR = 1.214, 95% CI = 0.936-1.574, P = 0.144). Because we could not exclude a small effect size due to a limited sample size, we further analyzed imputed rs2735383 genotypes (r(2) > 0.999) of 47,640 breast cancer cases and 46,656 controls from the Breast Cancer Association Consortium (BCAC). However, rs2735383CC was not associated with overall breast cancer risk in European (OR = 1.014, 95% CI = 0.969-1.060, P = 0.556) nor in Asian women (OR = 0.998, 95% CI = 0.905-1.100, P = 0.961). Subgroup analyses by age, age at menarche, age at menopause, menopausal status, number of pregnancies, breast feeding, family history and receptor status also did not reveal a significant association. This study therefore does not support the involvement of the genotype at NBS1 rs2735383 in breast cancer susceptibility.

U2 - 10.1038/srep36874

DO - 10.1038/srep36874

M3 - Journal article

C2 - 27845421

SN - 2045-2322

VL - 6

JO - Scientific Reports

JF - Scientific Reports

M1 - 36874

ER -

rs2735383, located at a microRNA binding site in the 3'UTR of NBS1, is not associated with breast cancer risk

Abstract

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