Role of phospholipase A2 in the induction of drip loss in porcine muscle.

Kristian A Poulsen; Jette F Young; Peter Theil; Miriam Kolko; Niels Oksbjerg; Ian H Lambert

doi:10.1021/jf062341n

Role of phospholipase A2 in the induction of drip loss in porcine muscle.

Kristian A Poulsen, Jette F Young, Peter Theil, Miriam Kolko, Niels Oksbjerg, Ian H Lambert

Cell Biology and Physiology

8 Citations (Scopus)

Abstract

The role of phospholipase A2 in the induction of drip loss from pig muscle has been investigated. In samples from porcine M. longissimus dorsi, total PLA2 activity as well as mRNA and protein levels of the group VIA iPLA2 (iPLA2-VIA) increased during the initial 4 h post-mortem period. Morphological studies of porcine muscle showed that at 4 h post-mortem, gaps had formed between muscle fibers and that the sarcolemma membrane borders appeared blurred. At the same time iPLA2-VIA protein levels were increased inside muscle fibers and at the sarcolemma. iPLA2-VIA mRNA abundance in samples from different breeds of pigs with variations in drip loss revealed no clear correlation between drip loss level and iPLA2-VIA expression. Together, these data indicate that during the post-mortem period, iPLA2-VIA expression and activity is increased at the muscle fiber membranes. PLA2 activity may affect membrane permeability and consequently the progression of drip formation in porcine muscle.

Original language	English
Journal	Journal of Agricultural and Food Chemistry
Volume	55
Issue number	5
Pages (from-to)	1970-6
Number of pages	6
ISSN	0021-8561
DOIs	https://doi.org/10.1021/jf062341n
Publication status	Published - 2007

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@article{c2c3c4902e3f11ddb7b4000ea68e967b,

title = "Role of phospholipase A2 in the induction of drip loss in porcine muscle.",

abstract = "The role of phospholipase A2 in the induction of drip loss from pig muscle has been investigated. In samples from porcine M. longissimus dorsi, total PLA2 activity as well as mRNA and protein levels of the group VIA iPLA2 (iPLA2-VIA) increased during the initial 4 h post-mortem period. Morphological studies of porcine muscle showed that at 4 h post-mortem, gaps had formed between muscle fibers and that the sarcolemma membrane borders appeared blurred. At the same time iPLA2-VIA protein levels were increased inside muscle fibers and at the sarcolemma. iPLA2-VIA mRNA abundance in samples from different breeds of pigs with variations in drip loss revealed no clear correlation between drip loss level and iPLA2-VIA expression. Together, these data indicate that during the post-mortem period, iPLA2-VIA expression and activity is increased at the muscle fiber membranes. PLA2 activity may affect membrane permeability and consequently the progression of drip formation in porcine muscle.",

author = "Poulsen, {Kristian A} and Young, {Jette F} and Peter Theil and Miriam Kolko and Niels Oksbjerg and Lambert, {Ian H}",

note = "Keywords: Animals; Body Water; Hydrogen-Ion Concentration; Muscle, Skeletal; Phospholipases A; Phospholipases A2; Postmortem Changes; RNA, Messenger; Swine",

year = "2007",

doi = "10.1021/jf062341n",

language = "English",

volume = "55",

pages = "1970--6",

journal = "Journal of Agricultural and Food Chemistry",

issn = "0021-8561",

publisher = "American Chemical Society",

number = "5",

}

TY - JOUR

T1 - Role of phospholipase A2 in the induction of drip loss in porcine muscle.

AU - Poulsen, Kristian A

AU - Young, Jette F

AU - Theil, Peter

AU - Kolko, Miriam

AU - Oksbjerg, Niels

AU - Lambert, Ian H

N1 - Keywords: Animals; Body Water; Hydrogen-Ion Concentration; Muscle, Skeletal; Phospholipases A; Phospholipases A2; Postmortem Changes; RNA, Messenger; Swine

PY - 2007

Y1 - 2007

N2 - The role of phospholipase A2 in the induction of drip loss from pig muscle has been investigated. In samples from porcine M. longissimus dorsi, total PLA2 activity as well as mRNA and protein levels of the group VIA iPLA2 (iPLA2-VIA) increased during the initial 4 h post-mortem period. Morphological studies of porcine muscle showed that at 4 h post-mortem, gaps had formed between muscle fibers and that the sarcolemma membrane borders appeared blurred. At the same time iPLA2-VIA protein levels were increased inside muscle fibers and at the sarcolemma. iPLA2-VIA mRNA abundance in samples from different breeds of pigs with variations in drip loss revealed no clear correlation between drip loss level and iPLA2-VIA expression. Together, these data indicate that during the post-mortem period, iPLA2-VIA expression and activity is increased at the muscle fiber membranes. PLA2 activity may affect membrane permeability and consequently the progression of drip formation in porcine muscle.

AB - The role of phospholipase A2 in the induction of drip loss from pig muscle has been investigated. In samples from porcine M. longissimus dorsi, total PLA2 activity as well as mRNA and protein levels of the group VIA iPLA2 (iPLA2-VIA) increased during the initial 4 h post-mortem period. Morphological studies of porcine muscle showed that at 4 h post-mortem, gaps had formed between muscle fibers and that the sarcolemma membrane borders appeared blurred. At the same time iPLA2-VIA protein levels were increased inside muscle fibers and at the sarcolemma. iPLA2-VIA mRNA abundance in samples from different breeds of pigs with variations in drip loss revealed no clear correlation between drip loss level and iPLA2-VIA expression. Together, these data indicate that during the post-mortem period, iPLA2-VIA expression and activity is increased at the muscle fiber membranes. PLA2 activity may affect membrane permeability and consequently the progression of drip formation in porcine muscle.

U2 - 10.1021/jf062341n

DO - 10.1021/jf062341n

M3 - Journal article

C2 - 17288434

SN - 0021-8561

VL - 55

SP - 1970

EP - 1976

JO - Journal of Agricultural and Food Chemistry

JF - Journal of Agricultural and Food Chemistry

IS - 5

ER -

Role of phospholipase A2 in the induction of drip loss in porcine muscle.

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