Roche genome sequencer FLX based high-throughput sequencing of ancient DNA

David E Alquezar-Planas; Sarah Louise Fordyce

doi:10.1007/978-1-61779-870-2_7

Roche genome sequencer FLX based high-throughput sequencing of ancient DNA

David E Alquezar-Planas, Sarah Louise Fordyce

Section of Forensic Genetics

Abstract

Since the development of so-called "next generation" high-throughput sequencing in 2005, this technology has been applied to a variety of fields. Such applications include disease studies, evolutionary investigations, and ancient DNA. Each application requires a specialized protocol to ensure that the data produced is optimal. Although much of the procedure can be followed directly from the manufacturer's protocols, the key differences lie in the library preparation steps. This chapter presents an optimized protocol for the sequencing of fossil remains and museum specimens, commonly referred to as "ancient DNA," using the Roche GS FLX 454 platform.

Original language	English
Journal	Methods in Molecular Biology
Volume	888
Pages (from-to)	109-18
Number of pages	10
ISSN	1064-3745
DOIs	https://doi.org/10.1007/978-1-61779-870-2_7
Publication status	Published - 2012

Keywords

Animals
Biological Specimen Banks
Chromosome Mapping
DNA
DNA Fragmentation
Extinction, Biological
Gene Library
Genome
High-Throughput Nucleotide Sequencing
Humans
Mammoths
Neanderthals
Nucleic Acid Amplification Techniques
Sequence Analysis, DNA
Ursidae

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10.1007/978-1-61779-870-2_7

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title = "Roche genome sequencer FLX based high-throughput sequencing of ancient DNA",

abstract = "Since the development of so-called {"}next generation{"} high-throughput sequencing in 2005, this technology has been applied to a variety of fields. Such applications include disease studies, evolutionary investigations, and ancient DNA. Each application requires a specialized protocol to ensure that the data produced is optimal. Although much of the procedure can be followed directly from the manufacturer's protocols, the key differences lie in the library preparation steps. This chapter presents an optimized protocol for the sequencing of fossil remains and museum specimens, commonly referred to as {"}ancient DNA,{"} using the Roche GS FLX 454 platform.",

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AU - Alquezar-Planas, David E

AU - Fordyce, Sarah Louise

PY - 2012

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N2 - Since the development of so-called "next generation" high-throughput sequencing in 2005, this technology has been applied to a variety of fields. Such applications include disease studies, evolutionary investigations, and ancient DNA. Each application requires a specialized protocol to ensure that the data produced is optimal. Although much of the procedure can be followed directly from the manufacturer's protocols, the key differences lie in the library preparation steps. This chapter presents an optimized protocol for the sequencing of fossil remains and museum specimens, commonly referred to as "ancient DNA," using the Roche GS FLX 454 platform.

AB - Since the development of so-called "next generation" high-throughput sequencing in 2005, this technology has been applied to a variety of fields. Such applications include disease studies, evolutionary investigations, and ancient DNA. Each application requires a specialized protocol to ensure that the data produced is optimal. Although much of the procedure can be followed directly from the manufacturer's protocols, the key differences lie in the library preparation steps. This chapter presents an optimized protocol for the sequencing of fossil remains and museum specimens, commonly referred to as "ancient DNA," using the Roche GS FLX 454 platform.

KW - Animals

KW - Biological Specimen Banks

KW - Chromosome Mapping

KW - DNA

KW - DNA Fragmentation

KW - Extinction, Biological

KW - Gene Library

KW - Genome

KW - High-Throughput Nucleotide Sequencing

KW - Humans

KW - Mammoths

KW - Neanderthals

KW - Nucleic Acid Amplification Techniques

KW - Sequence Analysis, DNA

KW - Ursidae

U2 - 10.1007/978-1-61779-870-2_7

DO - 10.1007/978-1-61779-870-2_7

M3 - Journal article

C2 - 22665278

SN - 1064-3745

VL - 888

SP - 109

EP - 118

JO - Methods in Molecular Biology

JF - Methods in Molecular Biology

ER -

Roche genome sequencer FLX based high-throughput sequencing of ancient DNA

Abstract

Keywords

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