RNA preparation and characterization for gene expression studies

Michael Stangegaard

doi:10.1007/978-1-59745-538-1_8

RNA preparation and characterization for gene expression studies

Michael Stangegaard

Section of Forensic Genetics

Abstract

Much information can be obtained from knowledge of the relative expression level of each gene in the transcriptome. With the current advances in technology as little as a single cell is required as starting material for gene expression experiments. The mRNA from a single cell may be linearly amplified to an excess of 10(6)-fold. Reverse transcription and fluorescent labeling of the amplified RNA yields a stable target for subsequent hybridization to DNA microarrays.

Original language	English
Title of host publication	Methods in Molecular Biology : DNA Microarrays for Biomedical Research
Editors	Hans Martin Dufva
Number of pages	17
Volume	529
Place of Publication	New York, NY
Publisher	Springer Science+Business Media
Publication date	2009
Edition	1
Pages	115-132
ISBN (Print)	1064-3745, 978-1-934115-69-5
ISBN (Electronic)	1940-6029, 978-1-59745-538-1
DOIs	https://doi.org/10.1007/978-1-59745-538-1_8
Publication status	Published - 2009

Keywords

Faculty of Social Sciences

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10.1007/978-1-59745-538-1_8

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RNA preparation and characterization for gene expression studies

Abstract

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