Risk evaluation of the Arctic environmental POP exposure based on critical body residue and critical daily dose using captive Greenland sledge dogs (Canis familiaris) as surrogate species

Christian Sonne; Kim Gustavson; Igor Eulaers; Jean-Pierre Desforges; Robert J Letcher; Frank F Rigét; Bjarne Styrishave; Rune Dietz

doi:10.1016/j.envint.2015.11.017

Risk evaluation of the Arctic environmental POP exposure based on critical body residue and critical daily dose using captive Greenland sledge dogs (Canis familiaris) as surrogate species

Christian Sonne, Kim Gustavson, Igor Eulaers, Jean-Pierre Desforges, Robert J Letcher, Frank F Rigét, Bjarne Styrishave, Rune Dietz

8 Citations (Scopus)

Abstract

The risk from POP (persistent organic pollutant) exposure and subsequent reproductive, immunotoxic and liver histopathological effects was evaluated in a classical parallel trial on Greenland sledge dogs (Canis familiaris) fed contaminated minke whale (Balaenoptera acutorostrata) blubber. First the critical body residues (CBRs) were estimated using the physiologically-based pharmacokinetic (PBPK) model for seven POP compounds based on rat critical daily doses (CDDs). These were then compared with the actual daily oral POP doses (DD) and body residues (BR) in the sledge dogs by calculating risk quotients (RQ_DD: DD/CDD; RQ_BR: BR/CBR; ≥1 indicates risk). The results showed that risk quotients for reproductive, immunotoxic and liver histopathological effects were significantly lowest in the control group (p<0.01) while risk quotients based on daily doses (RQ_DD) were significantly lower than RQs based on body residues (RQ_BR) (all p<0.01). RQ_BR in the exposed group ranged from 1.0-12 for reproductive and immunotoxic effects while those for liver histopathological effects ranged from 0.7-3.0. PCBs (polychlorinated biphenyls) and chlordanes were the dominant driver behind high immune and reproductive RQs while dieldrin was the most important factor behind RQs for liver histopathology. Principal component analyses and Spearman rank correlation analyses showed that complement and cellular immune parameters were significantly negative correlated with RQ_BR (all p<0.05) while logistic regression showed that RQ_DD had a significant effect on the number of born cups (p=0.03). No significantly relations were found between RQs and hormone concentrations, number of gestations, antibody titres or liver histopathology. These results confirm previous studies showing that POP exposure negatively impacts steroid hormones, various immune parameters, as well as liver histopathology in sledge dogs. It is also clear that RQ_BR is the best reflector of health effects from POP exposure and that it is especially accurate in predicting immune and reproductive effects. We recommend that PBPK modelled (CBR) and RQ_BR should be used in the assessment of POP exposure and health effects in Arctic top predators.

Original language	English
Journal	Environment International
Volume	88
Pages (from-to)	221-7
Number of pages	7
ISSN	0160-4120
DOIs	https://doi.org/10.1016/j.envint.2015.11.017
Publication status	Published - 1 Mar 2016

Keywords

Adipose Tissue
Animals
Arctic Regions
Dogs
Environmental Exposure
Environmental Pollutants
Female
Greenland
Liver
Minke Whale
Organic Chemicals
Random Allocation
Reproduction
Risk Assessment
Journal Article
Research Support, Non-U.S. Gov't

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10.1016/j.envint.2015.11.017

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Sonne, C., Gustavson, K., Eulaers, I., Desforges, J.-P., Letcher, R. J., Rigét, F. F., Styrishave, B., & Dietz, R. (2016). Risk evaluation of the Arctic environmental POP exposure based on critical body residue and critical daily dose using captive Greenland sledge dogs (Canis familiaris) as surrogate species. Environment International, 88, 221-7. https://doi.org/10.1016/j.envint.2015.11.017

Risk evaluation of the Arctic environmental POP exposure based on critical body residue and critical daily dose using captive Greenland sledge dogs (Canis familiaris) as surrogate species. / Sonne, Christian; Gustavson, Kim; Eulaers, Igor et al.
In: Environment International, Vol. 88, 01.03.2016, p. 221-7.

Research output: Contribution to journal › Journal article › Research › peer-review

Sonne, C, Gustavson, K, Eulaers, I, Desforges, J-P, Letcher, RJ, Rigét, FF, Styrishave, B & Dietz, R 2016, 'Risk evaluation of the Arctic environmental POP exposure based on critical body residue and critical daily dose using captive Greenland sledge dogs (Canis familiaris) as surrogate species', Environment International, vol. 88, pp. 221-7. https://doi.org/10.1016/j.envint.2015.11.017

@article{3ac1e69c91544d14883eab78ef523959,

title = "Risk evaluation of the Arctic environmental POP exposure based on critical body residue and critical daily dose using captive Greenland sledge dogs (Canis familiaris) as surrogate species",

abstract = "The risk from POP (persistent organic pollutant) exposure and subsequent reproductive, immunotoxic and liver histopathological effects was evaluated in a classical parallel trial on Greenland sledge dogs (Canis familiaris) fed contaminated minke whale (Balaenoptera acutorostrata) blubber. First the critical body residues (CBRs) were estimated using the physiologically-based pharmacokinetic (PBPK) model for seven POP compounds based on rat critical daily doses (CDDs). These were then compared with the actual daily oral POP doses (DD) and body residues (BR) in the sledge dogs by calculating risk quotients (RQDD: DD/CDD; RQBR: BR/CBR; ≥1 indicates risk). The results showed that risk quotients for reproductive, immunotoxic and liver histopathological effects were significantly lowest in the control group (p<0.01) while risk quotients based on daily doses (RQDD) were significantly lower than RQs based on body residues (RQBR) (all p<0.01). RQBR in the exposed group ranged from 1.0-12 for reproductive and immunotoxic effects while those for liver histopathological effects ranged from 0.7-3.0. PCBs (polychlorinated biphenyls) and chlordanes were the dominant driver behind high immune and reproductive RQs while dieldrin was the most important factor behind RQs for liver histopathology. Principal component analyses and Spearman rank correlation analyses showed that complement and cellular immune parameters were significantly negative correlated with RQBR (all p<0.05) while logistic regression showed that RQDD had a significant effect on the number of born cups (p=0.03). No significantly relations were found between RQs and hormone concentrations, number of gestations, antibody titres or liver histopathology. These results confirm previous studies showing that POP exposure negatively impacts steroid hormones, various immune parameters, as well as liver histopathology in sledge dogs. It is also clear that RQBR is the best reflector of health effects from POP exposure and that it is especially accurate in predicting immune and reproductive effects. We recommend that PBPK modelled (CBR) and RQBR should be used in the assessment of POP exposure and health effects in Arctic top predators.",

keywords = "Adipose Tissue, Animals, Arctic Regions, Dogs, Environmental Exposure, Environmental Pollutants, Female, Greenland, Liver, Minke Whale, Organic Chemicals, Random Allocation, Reproduction, Risk Assessment, Journal Article, Research Support, Non-U.S. Gov't",

author = "Christian Sonne and Kim Gustavson and Igor Eulaers and Jean-Pierre Desforges and Letcher, {Robert J} and Rig{\'e}t, {Frank F} and Bjarne Styrishave and Rune Dietz",

year = "2016",

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doi = "10.1016/j.envint.2015.11.017",

language = "English",

volume = "88",

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journal = "Environment International",

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T1 - Risk evaluation of the Arctic environmental POP exposure based on critical body residue and critical daily dose using captive Greenland sledge dogs (Canis familiaris) as surrogate species

AU - Sonne, Christian

AU - Gustavson, Kim

AU - Eulaers, Igor

AU - Desforges, Jean-Pierre

AU - Letcher, Robert J

AU - Rigét, Frank F

AU - Styrishave, Bjarne

AU - Dietz, Rune

PY - 2016/3/1

Y1 - 2016/3/1

N2 - The risk from POP (persistent organic pollutant) exposure and subsequent reproductive, immunotoxic and liver histopathological effects was evaluated in a classical parallel trial on Greenland sledge dogs (Canis familiaris) fed contaminated minke whale (Balaenoptera acutorostrata) blubber. First the critical body residues (CBRs) were estimated using the physiologically-based pharmacokinetic (PBPK) model for seven POP compounds based on rat critical daily doses (CDDs). These were then compared with the actual daily oral POP doses (DD) and body residues (BR) in the sledge dogs by calculating risk quotients (RQDD: DD/CDD; RQBR: BR/CBR; ≥1 indicates risk). The results showed that risk quotients for reproductive, immunotoxic and liver histopathological effects were significantly lowest in the control group (p<0.01) while risk quotients based on daily doses (RQDD) were significantly lower than RQs based on body residues (RQBR) (all p<0.01). RQBR in the exposed group ranged from 1.0-12 for reproductive and immunotoxic effects while those for liver histopathological effects ranged from 0.7-3.0. PCBs (polychlorinated biphenyls) and chlordanes were the dominant driver behind high immune and reproductive RQs while dieldrin was the most important factor behind RQs for liver histopathology. Principal component analyses and Spearman rank correlation analyses showed that complement and cellular immune parameters were significantly negative correlated with RQBR (all p<0.05) while logistic regression showed that RQDD had a significant effect on the number of born cups (p=0.03). No significantly relations were found between RQs and hormone concentrations, number of gestations, antibody titres or liver histopathology. These results confirm previous studies showing that POP exposure negatively impacts steroid hormones, various immune parameters, as well as liver histopathology in sledge dogs. It is also clear that RQBR is the best reflector of health effects from POP exposure and that it is especially accurate in predicting immune and reproductive effects. We recommend that PBPK modelled (CBR) and RQBR should be used in the assessment of POP exposure and health effects in Arctic top predators.

AB - The risk from POP (persistent organic pollutant) exposure and subsequent reproductive, immunotoxic and liver histopathological effects was evaluated in a classical parallel trial on Greenland sledge dogs (Canis familiaris) fed contaminated minke whale (Balaenoptera acutorostrata) blubber. First the critical body residues (CBRs) were estimated using the physiologically-based pharmacokinetic (PBPK) model for seven POP compounds based on rat critical daily doses (CDDs). These were then compared with the actual daily oral POP doses (DD) and body residues (BR) in the sledge dogs by calculating risk quotients (RQDD: DD/CDD; RQBR: BR/CBR; ≥1 indicates risk). The results showed that risk quotients for reproductive, immunotoxic and liver histopathological effects were significantly lowest in the control group (p<0.01) while risk quotients based on daily doses (RQDD) were significantly lower than RQs based on body residues (RQBR) (all p<0.01). RQBR in the exposed group ranged from 1.0-12 for reproductive and immunotoxic effects while those for liver histopathological effects ranged from 0.7-3.0. PCBs (polychlorinated biphenyls) and chlordanes were the dominant driver behind high immune and reproductive RQs while dieldrin was the most important factor behind RQs for liver histopathology. Principal component analyses and Spearman rank correlation analyses showed that complement and cellular immune parameters were significantly negative correlated with RQBR (all p<0.05) while logistic regression showed that RQDD had a significant effect on the number of born cups (p=0.03). No significantly relations were found between RQs and hormone concentrations, number of gestations, antibody titres or liver histopathology. These results confirm previous studies showing that POP exposure negatively impacts steroid hormones, various immune parameters, as well as liver histopathology in sledge dogs. It is also clear that RQBR is the best reflector of health effects from POP exposure and that it is especially accurate in predicting immune and reproductive effects. We recommend that PBPK modelled (CBR) and RQBR should be used in the assessment of POP exposure and health effects in Arctic top predators.

KW - Adipose Tissue

KW - Animals

KW - Arctic Regions

KW - Dogs

KW - Environmental Exposure

KW - Environmental Pollutants

KW - Female

KW - Greenland

KW - Liver

KW - Minke Whale

KW - Organic Chemicals

KW - Random Allocation

KW - Reproduction

KW - Risk Assessment

KW - Journal Article

KW - Research Support, Non-U.S. Gov't

U2 - 10.1016/j.envint.2015.11.017

DO - 10.1016/j.envint.2015.11.017

M3 - Journal article

C2 - 26773392

SN - 0160-4120

VL - 88

SP - 221

EP - 227

JO - Environment International

JF - Environment International

ER -

Risk evaluation of the Arctic environmental POP exposure based on critical body residue and critical daily dose using captive Greenland sledge dogs (Canis familiaris) as surrogate species

Abstract

Keywords

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