Abstract
The RNase III family of endoribonucleases participates in maturation and decay of cellular and viral transcripts by processing of double-stranded RNA. RNase III degradation is inherent to most, antisense RNA-regulated gene systems in Escherichia coil, In the hok/sok system from plasmid RI, Sok antisense RNA targets the holt mRNA for RNase III-mediated degradation. An intermediate in the pairing reaction between Sok RNA and hoh mRNA forms a three-way junction. A complex between a chimeric antisense RNA and hok mRNA that mimics the three-way junction was cleaved by RNase III both in vivo and in vitro. Footprinting using E117A RNase III binding to partially complementary RNAs showed protection of the 13 base pairs of interstrand duplex and of the bottom part of the transcriptional terminator hairpin of the antisense RNA. This suggests that the 13 base pairs of RNA duplex are coaxially stacked on the antisense RNA terminator stem-loop and that each stem forms a monomer half-site, allowing symmetrical binding of the RNase III dimer. This processing scheme shows an unanticipated diversity in RNase III substrates and may have a more general implication for RNA metabolism.
| Original language | English |
|---|---|
| Journal | Journal of Biological Chemistry |
| Volume | 274 |
| Issue number | 37 |
| Pages (from-to) | 26572-26578 |
| Number of pages | 7 |
| ISSN | 0021-9258 |
| DOIs | |
| Publication status | Published - 1999 |
| Externally published | Yes |