Revisiting bovine pyometra - new insights into the disease using a culture-independent deep seguencing approach

Lif Rødtness Vesterby Knudsen; Cecilia Christensen Karstrup; Hanne Gervi Pedersen; Jørgen Steen Agerholm; Tim Kåre Jensen; Kirstine Klitgaard Schou

doi:10.1016/j.vetmic.2014.12.006

Revisiting bovine pyometra - new insights into the disease using a culture-independent deep seguencing approach

Lif Rødtness Vesterby Knudsen, Cecilia Christensen Karstrup, Hanne Gervi Pedersen, Jørgen Steen Agerholm, Tim Kåre Jensen, Kirstine Klitgaard Schou

24 Citations (Scopus)

Abstract

The bacteria present in the uterus during pyometra have previously been studied using bacteriological culturing. These studies identified Fusobacterium necrophorum and Trueperella pyogenes as the major contributors to the pathogenesis of pyometra. However, an increasing number of culture-independent studies have demonstrated that the bacterial diversity in most environments is underestimated in culture-based studies. Consequently, fastidious pyometra-associated pathogens may have been overlooked. Therefore, the primary purpose of this study was to investigate the diversity of bacteria in the uterus of cows with pyometra by using culture-independent 16S rRNA PCR combined with next generation sequencing.We investigated the microbial composition in the uterus of 21 cows with pyometra, which were obtained from a Danish slaughterhouse. Similar to the observations from the culture studies, Fusobacteriaceae, the family that F. necrophorum belongs to, was the operational taxonomic unit (OTU) observed in the largest quantities. By contrast, the Actinomycetaceae family, which includes T. pyogenes, constituted only 1% of the total number of reads. Thus we cannot confirm the previously reported role of species from this family in the pathogenesis of pyometra. Finally, we identified a large number of sequences representing three families of Gram-negative bacteria in the pyometra samples: Porphyromonadaceae, Mycoplasmataceae, and Pasteurellaceae. It is likely that these families comprise potential pathogenic species of a fastidious nature, which have been overlooked in previous studies. Our results increase the knowledge of the complexity of the pyometra microbiota and suggest that pathogens in addition to F. necrophorum may be involved in the pathogenesis of pyometra.

Original language	English
Journal	Veterinary Microbiology
Volume	175
Pages (from-to)	319-324
Number of pages	6
ISSN	0378-1135
DOIs	https://doi.org/10.1016/j.vetmic.2014.12.006
Publication status	Published - 2015

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10.1016/j.vetmic.2014.12.006

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title = "Revisiting bovine pyometra - new insights into the disease using a culture-independent deep seguencing approach",

abstract = "The bacteria present in the uterus during pyometra have previously been studied using bacteriological culturing. These studies identified Fusobacterium necrophorum and Trueperella pyogenes as the major contributors to the pathogenesis of pyometra. However, an increasing number of culture-independent studies have demonstrated that the bacterial diversity in most environments is underestimated in culture-based studies. Consequently, fastidious pyometra-associated pathogens may have been overlooked. Therefore, the primary purpose of this study was to investigate the diversity of bacteria in the uterus of cows with pyometra by using culture-independent 16S rRNA PCR combined with next generation sequencing.We investigated the microbial composition in the uterus of 21 cows with pyometra, which were obtained from a Danish slaughterhouse. Similar to the observations from the culture studies, Fusobacteriaceae, the family that F. necrophorum belongs to, was the operational taxonomic unit (OTU) observed in the largest quantities. By contrast, the Actinomycetaceae family, which includes T. pyogenes, constituted only 1% of the total number of reads. Thus we cannot confirm the previously reported role of species from this family in the pathogenesis of pyometra. Finally, we identified a large number of sequences representing three families of Gram-negative bacteria in the pyometra samples: Porphyromonadaceae, Mycoplasmataceae, and Pasteurellaceae. It is likely that these families comprise potential pathogenic species of a fastidious nature, which have been overlooked in previous studies. Our results increase the knowledge of the complexity of the pyometra microbiota and suggest that pathogens in addition to F. necrophorum may be involved in the pathogenesis of pyometra.",

author = "{R{\o}dtness Vesterby Knudsen}, Lif and Karstrup, {Cecilia Christensen} and Pedersen, {Hanne Gervi} and Agerholm, {J{\o}rgen Steen} and Jensen, {Tim K{\aa}re} and Schou, {Kirstine Klitgaard}",

note = "Hej Jeg kan ikke frems{\o}ge tidskriftet?? Med venlig hilsen Michala Barrett Levinsen",

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T1 - Revisiting bovine pyometra - new insights into the disease using a culture-independent deep seguencing approach

AU - Rødtness Vesterby Knudsen, Lif

AU - Karstrup, Cecilia Christensen

AU - Pedersen, Hanne Gervi

AU - Agerholm, Jørgen Steen

AU - Jensen, Tim Kåre

AU - Schou, Kirstine Klitgaard

N1 - Hej Jeg kan ikke fremsøge tidskriftet?? Med venlig hilsen Michala Barrett Levinsen

PY - 2015

Y1 - 2015

N2 - The bacteria present in the uterus during pyometra have previously been studied using bacteriological culturing. These studies identified Fusobacterium necrophorum and Trueperella pyogenes as the major contributors to the pathogenesis of pyometra. However, an increasing number of culture-independent studies have demonstrated that the bacterial diversity in most environments is underestimated in culture-based studies. Consequently, fastidious pyometra-associated pathogens may have been overlooked. Therefore, the primary purpose of this study was to investigate the diversity of bacteria in the uterus of cows with pyometra by using culture-independent 16S rRNA PCR combined with next generation sequencing.We investigated the microbial composition in the uterus of 21 cows with pyometra, which were obtained from a Danish slaughterhouse. Similar to the observations from the culture studies, Fusobacteriaceae, the family that F. necrophorum belongs to, was the operational taxonomic unit (OTU) observed in the largest quantities. By contrast, the Actinomycetaceae family, which includes T. pyogenes, constituted only 1% of the total number of reads. Thus we cannot confirm the previously reported role of species from this family in the pathogenesis of pyometra. Finally, we identified a large number of sequences representing three families of Gram-negative bacteria in the pyometra samples: Porphyromonadaceae, Mycoplasmataceae, and Pasteurellaceae. It is likely that these families comprise potential pathogenic species of a fastidious nature, which have been overlooked in previous studies. Our results increase the knowledge of the complexity of the pyometra microbiota and suggest that pathogens in addition to F. necrophorum may be involved in the pathogenesis of pyometra.

AB - The bacteria present in the uterus during pyometra have previously been studied using bacteriological culturing. These studies identified Fusobacterium necrophorum and Trueperella pyogenes as the major contributors to the pathogenesis of pyometra. However, an increasing number of culture-independent studies have demonstrated that the bacterial diversity in most environments is underestimated in culture-based studies. Consequently, fastidious pyometra-associated pathogens may have been overlooked. Therefore, the primary purpose of this study was to investigate the diversity of bacteria in the uterus of cows with pyometra by using culture-independent 16S rRNA PCR combined with next generation sequencing.We investigated the microbial composition in the uterus of 21 cows with pyometra, which were obtained from a Danish slaughterhouse. Similar to the observations from the culture studies, Fusobacteriaceae, the family that F. necrophorum belongs to, was the operational taxonomic unit (OTU) observed in the largest quantities. By contrast, the Actinomycetaceae family, which includes T. pyogenes, constituted only 1% of the total number of reads. Thus we cannot confirm the previously reported role of species from this family in the pathogenesis of pyometra. Finally, we identified a large number of sequences representing three families of Gram-negative bacteria in the pyometra samples: Porphyromonadaceae, Mycoplasmataceae, and Pasteurellaceae. It is likely that these families comprise potential pathogenic species of a fastidious nature, which have been overlooked in previous studies. Our results increase the knowledge of the complexity of the pyometra microbiota and suggest that pathogens in addition to F. necrophorum may be involved in the pathogenesis of pyometra.

U2 - 10.1016/j.vetmic.2014.12.006

DO - 10.1016/j.vetmic.2014.12.006

M3 - Journal article

C2 - 25550285

SN - 0378-1135

VL - 175

SP - 319

EP - 324

JO - Veterinary Microbiology

JF - Veterinary Microbiology

ER -

Revisiting bovine pyometra - new insights into the disease using a culture-independent deep seguencing approach

Abstract

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