Retinal pigment epithelial cells upregulate expression of complement factors after co-culture with activated T cells

TY - JOUR

T1 - Retinal pigment epithelial cells upregulate expression of complement factors after co-culture with activated T cells

AU - Juel, Helene Bæk

AU - Kaestel, Charlotte

AU - Folkersen, Lasse

AU - Faber, Carsten

AU - Heegaard, Niels Henrik Helweg

AU - Borup, Rehannah

AU - Nissen, Mogens Holst

PY - 2011/3

Y1 - 2011/3

N2 - In this study we examined the effect of T cell-derived cytokines on retinal pigment epithelial (RPE) cells with respect to expression of complement components. We used an in vitro co-culture system in which CD3/CD28-activated human T cells were separated from the human RPE cell line (ARPE-19) by a membrane. Differential gene expression in the RPE cells of complement factor genes was identified using gene arrays, and selected gene transcripts were validated by q-RT-PCR. Protein expression was determined by ELISA and immunoblotting. Co-culture with activated T cells increased RPE mRNA and/or protein expression of complement components C3, factors B, H, H-like 1, CD46, CD55, CD59, and clusterin, in a dose-dependent manner. Soluble factors derived from activated T cells are capable of increasing expression of complement components in RPE cells. This is important for the further understanding of inflammatory ocular diseases such as uveitis and age-related macular degeneration. --------------------------------------------------------------------------------

AB - In this study we examined the effect of T cell-derived cytokines on retinal pigment epithelial (RPE) cells with respect to expression of complement components. We used an in vitro co-culture system in which CD3/CD28-activated human T cells were separated from the human RPE cell line (ARPE-19) by a membrane. Differential gene expression in the RPE cells of complement factor genes was identified using gene arrays, and selected gene transcripts were validated by q-RT-PCR. Protein expression was determined by ELISA and immunoblotting. Co-culture with activated T cells increased RPE mRNA and/or protein expression of complement components C3, factors B, H, H-like 1, CD46, CD55, CD59, and clusterin, in a dose-dependent manner. Soluble factors derived from activated T cells are capable of increasing expression of complement components in RPE cells. This is important for the further understanding of inflammatory ocular diseases such as uveitis and age-related macular degeneration. --------------------------------------------------------------------------------

KW - Faculty of Health and Medical Sciences

KW - RPE

KW - komplement

KW - T celler

U2 - 10.1016/j.exer.2011.01.003

DO - 10.1016/j.exer.2011.01.003

M3 - Journal article

C2 - 21255569

SN - 0014-4835

VL - 92

SP - 180

EP - 188

JO - Experimental Eye Research

JF - Experimental Eye Research

IS - 3

ER -