Retinal basement membrane alterations in human diabetic retinopathy eyes

A. V. Ljubimov; R. E. Burgeson; R. J. Butkowski; J. R. Couchman; Z. Huang; A. B. Nesburn; M. C. Kenney

Retinal basement membrane alterations in human diabetic retinopathy eyes

A. V. Ljubimov^*, R. E. Burgeson, R. J. Butkowski, J. R. Couchman, Z. Huang, A. B. Nesburn, M. C. Kenney

^*Corresponding author for this work

Abstract

Purpose. To uncover extracellular matrix (ECM) and basement membrane (BM) alterations in retinas of diabetic retinopathy (DR) eyes. Methods. Cryostat sections of human autopsy eyes obtained from NDRI (6 normal, 5 diabetic without DR, and 12 diabetic with DR, including 7 IDDM and 5 NIDDM) were subjected to immunofluorescence with antibodies to 25 ECM and BM components. Results. Fibrotic alterations of the retinal BM, or inner limiting membrane (ILM), were found in DR retinas. Normal and non-DR diabetic ILM had laminin-4, α3-α5 type IV collagen, perlecan and entactin/nidogen. In most DR cases, ILM also stained strongly for fibronectin and types I, III, IV (α1-α2) and V collagen. In diabetic preretinal membranes (n=3), perlecan, bamacan, types VI, XII and XIV collagen were newly found. In areas of neovascularization (n=4), BMs of new blood vessels in DR retinas and preretinal membranes stained strongly for tenascin and type XII collagen. In retinal vessels of normal, diabetic and even adjacent DR retinas, only weak and irregular staining for both components could be revealed. Conclusions. Fibrotic changes of ILM in DR may lead to the isolation of retina from vitreous-derived growth inhibitors, and promote neovascularization and formation of preretinal membranes. BM changes in newly formed retinal blood vessels may reflect and/or contribute to the abnormalities of their structure (pericyte absence) and function.

Original language	English
Journal	Investigative Ophthalmology and Visual Science
Volume	37
Issue number	3
ISSN	0146-0404
Publication status	Published - 15 Feb 1996
Externally published	Yes

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title = "Retinal basement membrane alterations in human diabetic retinopathy eyes",

abstract = "Purpose. To uncover extracellular matrix (ECM) and basement membrane (BM) alterations in retinas of diabetic retinopathy (DR) eyes. Methods. Cryostat sections of human autopsy eyes obtained from NDRI (6 normal, 5 diabetic without DR, and 12 diabetic with DR, including 7 IDDM and 5 NIDDM) were subjected to immunofluorescence with antibodies to 25 ECM and BM components. Results. Fibrotic alterations of the retinal BM, or inner limiting membrane (ILM), were found in DR retinas. Normal and non-DR diabetic ILM had laminin-4, α3-α5 type IV collagen, perlecan and entactin/nidogen. In most DR cases, ILM also stained strongly for fibronectin and types I, III, IV (α1-α2) and V collagen. In diabetic preretinal membranes (n=3), perlecan, bamacan, types VI, XII and XIV collagen were newly found. In areas of neovascularization (n=4), BMs of new blood vessels in DR retinas and preretinal membranes stained strongly for tenascin and type XII collagen. In retinal vessels of normal, diabetic and even adjacent DR retinas, only weak and irregular staining for both components could be revealed. Conclusions. Fibrotic changes of ILM in DR may lead to the isolation of retina from vitreous-derived growth inhibitors, and promote neovascularization and formation of preretinal membranes. BM changes in newly formed retinal blood vessels may reflect and/or contribute to the abnormalities of their structure (pericyte absence) and function.",

author = "Ljubimov, {A. V.} and Burgeson, {R. E.} and Butkowski, {R. J.} and Couchman, {J. R.} and Z. Huang and Nesburn, {A. B.} and Kenney, {M. C.}",

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TY - JOUR

T1 - Retinal basement membrane alterations in human diabetic retinopathy eyes

AU - Ljubimov, A. V.

AU - Burgeson, R. E.

AU - Butkowski, R. J.

AU - Couchman, J. R.

AU - Huang, Z.

AU - Nesburn, A. B.

AU - Kenney, M. C.

PY - 1996/2/15

Y1 - 1996/2/15

N2 - Purpose. To uncover extracellular matrix (ECM) and basement membrane (BM) alterations in retinas of diabetic retinopathy (DR) eyes. Methods. Cryostat sections of human autopsy eyes obtained from NDRI (6 normal, 5 diabetic without DR, and 12 diabetic with DR, including 7 IDDM and 5 NIDDM) were subjected to immunofluorescence with antibodies to 25 ECM and BM components. Results. Fibrotic alterations of the retinal BM, or inner limiting membrane (ILM), were found in DR retinas. Normal and non-DR diabetic ILM had laminin-4, α3-α5 type IV collagen, perlecan and entactin/nidogen. In most DR cases, ILM also stained strongly for fibronectin and types I, III, IV (α1-α2) and V collagen. In diabetic preretinal membranes (n=3), perlecan, bamacan, types VI, XII and XIV collagen were newly found. In areas of neovascularization (n=4), BMs of new blood vessels in DR retinas and preretinal membranes stained strongly for tenascin and type XII collagen. In retinal vessels of normal, diabetic and even adjacent DR retinas, only weak and irregular staining for both components could be revealed. Conclusions. Fibrotic changes of ILM in DR may lead to the isolation of retina from vitreous-derived growth inhibitors, and promote neovascularization and formation of preretinal membranes. BM changes in newly formed retinal blood vessels may reflect and/or contribute to the abnormalities of their structure (pericyte absence) and function.

AB - Purpose. To uncover extracellular matrix (ECM) and basement membrane (BM) alterations in retinas of diabetic retinopathy (DR) eyes. Methods. Cryostat sections of human autopsy eyes obtained from NDRI (6 normal, 5 diabetic without DR, and 12 diabetic with DR, including 7 IDDM and 5 NIDDM) were subjected to immunofluorescence with antibodies to 25 ECM and BM components. Results. Fibrotic alterations of the retinal BM, or inner limiting membrane (ILM), were found in DR retinas. Normal and non-DR diabetic ILM had laminin-4, α3-α5 type IV collagen, perlecan and entactin/nidogen. In most DR cases, ILM also stained strongly for fibronectin and types I, III, IV (α1-α2) and V collagen. In diabetic preretinal membranes (n=3), perlecan, bamacan, types VI, XII and XIV collagen were newly found. In areas of neovascularization (n=4), BMs of new blood vessels in DR retinas and preretinal membranes stained strongly for tenascin and type XII collagen. In retinal vessels of normal, diabetic and even adjacent DR retinas, only weak and irregular staining for both components could be revealed. Conclusions. Fibrotic changes of ILM in DR may lead to the isolation of retina from vitreous-derived growth inhibitors, and promote neovascularization and formation of preretinal membranes. BM changes in newly formed retinal blood vessels may reflect and/or contribute to the abnormalities of their structure (pericyte absence) and function.

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M3 - Journal article

AN - SCOPUS:33750190969

SN - 0146-0404

VL - 37

JO - Investigative Ophthalmology and Visual Science

JF - Investigative Ophthalmology and Visual Science

IS - 3

ER -

Retinal basement membrane alterations in human diabetic retinopathy eyes

Abstract

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