Removal of Polysorbate 80 by complexation prior to LC-MS analysis

TY - JOUR

T1 - Removal of Polysorbate 80 by complexation prior to LC-MS analysis

AU - Jäpelt, Kristina Bak

AU - Johnsen, Lea Giørtz

AU - Christensen, Jan H.

PY - 2016/3/1

Y1 - 2016/3/1

N2 - The presence of Polysorbate 80 in samples can challenge liquid chromatography-electrospray ionisation mass spectrometry (LC-ESI-MS) analysis as it is easily ionised and detected. In this study, we demonstrate that interference from Polysorbate 80 can be reduced by complexation with a metal ion followed by precipitation by thiocyanate. The precipitation procedure was tested on a mixture of low molecular weight compounds (e.g. amino acids and non-amino organic acids) and it was shown that none of the tested compounds were precipitated.

AB - The presence of Polysorbate 80 in samples can challenge liquid chromatography-electrospray ionisation mass spectrometry (LC-ESI-MS) analysis as it is easily ionised and detected. In this study, we demonstrate that interference from Polysorbate 80 can be reduced by complexation with a metal ion followed by precipitation by thiocyanate. The precipitation procedure was tested on a mixture of low molecular weight compounds (e.g. amino acids and non-amino organic acids) and it was shown that none of the tested compounds were precipitated.

KW - Journal Article

KW - Research Support, Non-U.S. Gov't

U2 - 10.1007/s00216-016-9326-1

DO - 10.1007/s00216-016-9326-1

M3 - Journal article

C2 - 26894763

SN - 0365-4877

VL - 408

SP - 2303

EP - 2307

JO - Analusis

JF - Analusis

IS - 9

ER -