Regulation of ribosome synthesis in Tetrahymena pyriformis. 2. Coordination of synthesis of ribosomal proteins and ribosomal RNA during nutritional shift-up

TY - JOUR

T1 - Regulation of ribosome synthesis in Tetrahymena pyriformis. 2. Coordination of synthesis of ribosomal proteins and ribosomal RNA during nutritional shift-up

AU - Dreisig, H

AU - Andreasen, P H

AU - Kristiansen, K

N1 - Keywords: Animals; Culture Media; Dactinomycin; Kinetics; Polyribosomes; RNA, Ribosomal; Ribosomal Proteins; Ribosomes; Tetrahymena pyriformis

PY - 1984

Y1 - 1984

N2 - The addition of nutrients to long-time-starved cells of Tetrahymena pyriformis leads to a 50-60-fold increase in the rate of synthesis of ribosomal proteins (r-proteins). This is achieved by a 6-fold increase in the relative rate of r-protein synthesis and a 8-10-fold increase in the rate of total protein synthesis. Synthesis of r-proteins constitutes one third of total cellular protein synthesis 2-4 h after refeeding and the absolute rate of r-protein synthesis is approximately three-times greater than in exponentially growing cells. The synthesis of the individual r-proteins is coordinately regulated during a nutritional shift-up, and de novo synthesized r-proteins are stable. Addition of actinomycin D prevents the increase in the rate of r-protein synthesis. The rates of synthesis of rRNA and r-protein increase in concert, implying coordinate regulation. Furthermore, a comparison of the observed accumulation of r-proteins with the predicted accumulation based on the accumulation of rRNA suggests that rRNA and r-protein are synthesized in a stoichiometrically balanced way during the entire refeeding period.

AB - The addition of nutrients to long-time-starved cells of Tetrahymena pyriformis leads to a 50-60-fold increase in the rate of synthesis of ribosomal proteins (r-proteins). This is achieved by a 6-fold increase in the relative rate of r-protein synthesis and a 8-10-fold increase in the rate of total protein synthesis. Synthesis of r-proteins constitutes one third of total cellular protein synthesis 2-4 h after refeeding and the absolute rate of r-protein synthesis is approximately three-times greater than in exponentially growing cells. The synthesis of the individual r-proteins is coordinately regulated during a nutritional shift-up, and de novo synthesized r-proteins are stable. Addition of actinomycin D prevents the increase in the rate of r-protein synthesis. The rates of synthesis of rRNA and r-protein increase in concert, implying coordinate regulation. Furthermore, a comparison of the observed accumulation of r-proteins with the predicted accumulation based on the accumulation of rRNA suggests that rRNA and r-protein are synthesized in a stoichiometrically balanced way during the entire refeeding period.

U2 - 10.1111/j.1432-1033.1984.tb08127.x

DO - 10.1111/j.1432-1033.1984.tb08127.x

M3 - Journal article

C2 - 6426954

SN - 1742-464X

VL - 140

SP - 477

EP - 483

JO - FEBS Journal

JF - FEBS Journal

IS - 3

ER -