Regular cellular distribution of plasmids by oscillating and filament-forming ParA ATPase of plasmid pB171

Gitte Ebersbach; Simon Ringgaard; Jakob Moller-Jensen; Qing Wang; David J. Sherratt; Kenn Gerdes

doi:10.1111/j.1365-2958.2006.05322.x

Regular cellular distribution of plasmids by oscillating and filament-forming ParA ATPase of plasmid pB171

Gitte Ebersbach, Simon Ringgaard, Jakob Moller-Jensen, Qing Wang, David J. Sherratt, Kenn Gerdes

92 Citations (Scopus)

Abstract

Centromere-like loci from bacteria segregate plasmids to progeny cells before cell division. The ParA ATPase (a MinD homologue) of the par2 locus from plasmid pB171 forms oscillating helical structures over the nucleoid. Here we show that par2 distributes plasmid foci regularly along the length of the cell even in cells with many plasmids. In vitro, ParA binds ATP and ADP and has a cooperative ATPase activity. Moreover, ParA forms ATP-dependent filaments and cables, suggesting that ParA can provide the mechanical force for the observed regular distribution of plasmids. ParA and ParB interact with each other in a bacterial two-hybrid assay but do not interact with FtsZ, eight other essential cell division proteins or MreB actin. Based on these observations, we propose a simple model for how oscillating ParA filaments can mediate regular cellular distribution of plasmids. The model functions without the involvement of partition-specific host cell receptors and is thus consistent with the striking observation that partition loci can function in heterologous host organisms.

Original language	English
Journal	Molecular Microbiology
Volume	61
Issue number	6
Pages (from-to)	1428-1442
Number of pages	15
ISSN	0950-382X
DOIs	https://doi.org/10.1111/j.1365-2958.2006.05322.x
Publication status	Published - 2006
Externally published	Yes

Access to Document

10.1111/j.1365-2958.2006.05322.x

Cite this

@article{ec2aef75bc584c208230359c683b1381,

title = "Regular cellular distribution of plasmids by oscillating and filament-forming ParA ATPase of plasmid pB171",

abstract = "Centromere-like loci from bacteria segregate plasmids to progeny cells before cell division. The ParA ATPase (a MinD homologue) of the par2 locus from plasmid pB171 forms oscillating helical structures over the nucleoid. Here we show that par2 distributes plasmid foci regularly along the length of the cell even in cells with many plasmids. In vitro, ParA binds ATP and ADP and has a cooperative ATPase activity. Moreover, ParA forms ATP-dependent filaments and cables, suggesting that ParA can provide the mechanical force for the observed regular distribution of plasmids. ParA and ParB interact with each other in a bacterial two-hybrid assay but do not interact with FtsZ, eight other essential cell division proteins or MreB actin. Based on these observations, we propose a simple model for how oscillating ParA filaments can mediate regular cellular distribution of plasmids. The model functions without the involvement of partition-specific host cell receptors and is thus consistent with the striking observation that partition loci can function in heterologous host organisms.",

author = "Gitte Ebersbach and Simon Ringgaard and Jakob Moller-Jensen and Qing Wang and Sherratt, {David J.} and Kenn Gerdes",

year = "2006",

doi = "10.1111/j.1365-2958.2006.05322.x",

language = "English",

volume = "61",

pages = "1428--1442",

journal = "Molecular Microbiology",

issn = "0950-382X",

publisher = "Wiley-Blackwell",

number = "6",

}

TY - JOUR

T1 - Regular cellular distribution of plasmids by oscillating and filament-forming ParA ATPase of plasmid pB171

AU - Ebersbach, Gitte

AU - Ringgaard, Simon

AU - Moller-Jensen, Jakob

AU - Wang, Qing

AU - Sherratt, David J.

AU - Gerdes, Kenn

PY - 2006

Y1 - 2006

N2 - Centromere-like loci from bacteria segregate plasmids to progeny cells before cell division. The ParA ATPase (a MinD homologue) of the par2 locus from plasmid pB171 forms oscillating helical structures over the nucleoid. Here we show that par2 distributes plasmid foci regularly along the length of the cell even in cells with many plasmids. In vitro, ParA binds ATP and ADP and has a cooperative ATPase activity. Moreover, ParA forms ATP-dependent filaments and cables, suggesting that ParA can provide the mechanical force for the observed regular distribution of plasmids. ParA and ParB interact with each other in a bacterial two-hybrid assay but do not interact with FtsZ, eight other essential cell division proteins or MreB actin. Based on these observations, we propose a simple model for how oscillating ParA filaments can mediate regular cellular distribution of plasmids. The model functions without the involvement of partition-specific host cell receptors and is thus consistent with the striking observation that partition loci can function in heterologous host organisms.

AB - Centromere-like loci from bacteria segregate plasmids to progeny cells before cell division. The ParA ATPase (a MinD homologue) of the par2 locus from plasmid pB171 forms oscillating helical structures over the nucleoid. Here we show that par2 distributes plasmid foci regularly along the length of the cell even in cells with many plasmids. In vitro, ParA binds ATP and ADP and has a cooperative ATPase activity. Moreover, ParA forms ATP-dependent filaments and cables, suggesting that ParA can provide the mechanical force for the observed regular distribution of plasmids. ParA and ParB interact with each other in a bacterial two-hybrid assay but do not interact with FtsZ, eight other essential cell division proteins or MreB actin. Based on these observations, we propose a simple model for how oscillating ParA filaments can mediate regular cellular distribution of plasmids. The model functions without the involvement of partition-specific host cell receptors and is thus consistent with the striking observation that partition loci can function in heterologous host organisms.

U2 - 10.1111/j.1365-2958.2006.05322.x

DO - 10.1111/j.1365-2958.2006.05322.x

M3 - Journal article

C2 - 16899080

SN - 0950-382X

VL - 61

SP - 1428

EP - 1442

JO - Molecular Microbiology

JF - Molecular Microbiology

IS - 6

ER -