Region and cell-type resolved quantitative proteomic map of the human heart

TY - JOUR

T1 - Region and cell-type resolved quantitative proteomic map of the human heart

AU - Doll, Sophia

AU - Dreßen, Martina

AU - Geyer, Philipp E

AU - Itzhak, Daniel N

AU - Braun, Christian

AU - Doppler, Stefanie A

AU - Meier, Florian

AU - Deutsch, Marcus-Andre

AU - Lahm, Harald

AU - Lange, Rüdiger

AU - Krane, Markus

AU - Mann, Matthias

PY - 2017/12/1

Y1 - 2017/12/1

N2 - The heart is a central human organ and its diseases are the leading cause of death worldwide, but an in-depth knowledge of the identity and quantity of its constituent proteins is still lacking. Here, we determine the healthy human heart proteome by measuring 16 anatomical regions and three major cardiac cell types by high-resolution mass spectrometry-based proteomics. From low microgram sample amounts, we quantify over 10,700 proteins in this high dynamic range tissue. We combine copy numbers per cell with protein organellar assignments to build a model of the heart proteome at the subcellular level. Analysis of cardiac fibroblasts identifies cellular receptors as potential cell surface markers. Application of our heart map to atrial fibrillation reveals individually distinct mitochondrial dysfunctions. The heart map is available at maxqb.biochem.mpg.de as a resource for future analyses of normal heart function and disease.

AB - The heart is a central human organ and its diseases are the leading cause of death worldwide, but an in-depth knowledge of the identity and quantity of its constituent proteins is still lacking. Here, we determine the healthy human heart proteome by measuring 16 anatomical regions and three major cardiac cell types by high-resolution mass spectrometry-based proteomics. From low microgram sample amounts, we quantify over 10,700 proteins in this high dynamic range tissue. We combine copy numbers per cell with protein organellar assignments to build a model of the heart proteome at the subcellular level. Analysis of cardiac fibroblasts identifies cellular receptors as potential cell surface markers. Application of our heart map to atrial fibrillation reveals individually distinct mitochondrial dysfunctions. The heart map is available at maxqb.biochem.mpg.de as a resource for future analyses of normal heart function and disease.

KW - Journal Article

U2 - 10.1038/s41467-017-01747-2

DO - 10.1038/s41467-017-01747-2

M3 - Journal article

C2 - 29133944

SN - 2041-1723

VL - 8

JO - Nature Communications

JF - Nature Communications

IS - 1

M1 - 1469

ER -