Reduction of molecular gas diffusion through gaskets in leaf gas exchange cuvettes by leaf-mediated pores

TY - JOUR

T1 - Reduction of molecular gas diffusion through gaskets in leaf gas exchange cuvettes by leaf-mediated pores

AU - Boesgaard, Kristine Stove

AU - Mikkelsen, Teis Nørgaard

AU - Ro-Poulsen, Helge

AU - Ibrom, Andreas

N1 - CENPERMOA[2013]

PY - 2013/7

Y1 - 2013/7

N2 - There is an ongoing debate on how to correct leaf gas exchange measurements for the unavoidable diffusion leakage that occurs when measurements are done in non-ambient CO2 concentrations. In this study, we present a theory on how the CO2 diffusion gradient over the gasket is affected by leaf-mediated pores (LMP) and how LMP reduce diffusive exchange across the gaskets. Recent discussions have so far neglected the processes in the quasi-laminar boundary layer around the gasket. Counter intuitively, LMP reduce the leakage through gaskets, which can be explained by assuming that the boundary layer at the exterior of the cuvette is enriched with air from the inside of the cuvette. The effect can thus be reduced by reducing the boundary layer thickness. The theory clarifies conflicting results from earlier studies. We developed leaf adaptor frames that eliminate LMP during measurements on delicate plant material such as grass leaves with circular cross section, and the effectiveness is shown with respiration measurements on a harp of Deschampsia flexuosa leaves. We conclude that the best solution for measurements with portable photosynthesis systems is to avoid LMP rather than trying to correct for the effects. Abstract: Unavoidable diffusion leakage in portable photosynthesis systems is continually debated. This study introduces an improved theory on the diffusion leakage and concludes how other published studies within this area represent different parts of the presented theory. Avoiding leaf mediated pores between leaf cuvette gaskets are resulting in an unavoidable but stable diffusion rate, why it is concluded that available portable photosynthesis systems can be accurate measuring small fluxes, such as respiration.

AB - There is an ongoing debate on how to correct leaf gas exchange measurements for the unavoidable diffusion leakage that occurs when measurements are done in non-ambient CO2 concentrations. In this study, we present a theory on how the CO2 diffusion gradient over the gasket is affected by leaf-mediated pores (LMP) and how LMP reduce diffusive exchange across the gaskets. Recent discussions have so far neglected the processes in the quasi-laminar boundary layer around the gasket. Counter intuitively, LMP reduce the leakage through gaskets, which can be explained by assuming that the boundary layer at the exterior of the cuvette is enriched with air from the inside of the cuvette. The effect can thus be reduced by reducing the boundary layer thickness. The theory clarifies conflicting results from earlier studies. We developed leaf adaptor frames that eliminate LMP during measurements on delicate plant material such as grass leaves with circular cross section, and the effectiveness is shown with respiration measurements on a harp of Deschampsia flexuosa leaves. We conclude that the best solution for measurements with portable photosynthesis systems is to avoid LMP rather than trying to correct for the effects. Abstract: Unavoidable diffusion leakage in portable photosynthesis systems is continually debated. This study introduces an improved theory on the diffusion leakage and concludes how other published studies within this area represent different parts of the presented theory. Avoiding leaf mediated pores between leaf cuvette gaskets are resulting in an unavoidable but stable diffusion rate, why it is concluded that available portable photosynthesis systems can be accurate measuring small fluxes, such as respiration.

U2 - 10.1111/pce.12064

DO - 10.1111/pce.12064

M3 - Journal article

C2 - 23320654

SN - 0140-7791

VL - 36

SP - 1352

EP - 1362

JO - Plant, Cell and Environment

JF - Plant, Cell and Environment

IS - 7

ER -