Reduced cannabinoid receptor 1 protein in subcutaneous adipose tissue of obese

Marianne Faurholt Bennetzen; Thomas Svava Nielsen; S K Paulsen; J Bendix; S Fisker; N Jessen; S Lund; Bjørn Richelsen; S B Pedersen

doi:10.1111/j.1365-2362.2009.02231.x

Reduced cannabinoid receptor 1 protein in subcutaneous adipose tissue of obese

Marianne Faurholt Bennetzen, Thomas Svava Nielsen, S K Paulsen, J Bendix, S Fisker, N Jessen, S Lund, Bjørn Richelsen, S B Pedersen

16 Citations (Scopus)

Abstract

Background Cannabinoid 1 receptors are identified in various tissues involved in the internal metabolism including adipose tissue and the endocannabinoid system is claimed to be overactive in the obese state. To study the potential involvement of cannabinoid receptor 1 in the endocannabinoid system over-activity in adipose tissue in the obese state, we investigated the cannabinoid receptor 1 levels in adipose tissue from different fat depots in lean and obese humans. Materials and methods The adipose tissue samples were analysed by Western blot and by RT-PCR. Results Both the gene expression and the protein of cannabinoid receptor 1 were lower in subcutaneous abdominal adipose tissue from obese subjects as compared with lean subjects (P < 0·01 and P = 0·058). Moreover, in lean subjects, the level of cannabinoid receptor 1 was significantly higher in subcutaneous adipose tissue compared with visceral adipose tissue (P < 0·05) for both gene expression and protein. The level of cannabinoid receptor 1 was similar between the two depots in obese subjects. The expression of cannabinoid receptor 1 was higher in subcutaneous gluteal adipose tissue as compared with subcutaneous abdominal adipose tissue (P < 0·05). Conclusion We found in lean subjects, a robust lower level of cannabinoid receptor 1 in visceral adipose tissue compared with subcutaneous adipose tissue (both RNA and protein levels), but similar levels of cannabinoid receptor 1 between the two depots in obese subjects. Our present findings do not indicate that cannabinoid receptor 1 is directly involved in the endocannabinoid system over-activity in adipose tissue in obesity.

Original language	English
Journal	European Journal of Clinical Investigation
Volume	40
Issue number	2
Pages (from-to)	121-6
Number of pages	6
ISSN	0014-2972
DOIs	https://doi.org/10.1111/j.1365-2362.2009.02231.x
Publication status	Published - Feb 2010
Externally published	Yes

Keywords

Adipocytes
Adult
Blotting, Western
Female
Humans
Intra-Abdominal Fat
Male
Middle Aged
Obesity
RNA, Messenger
Receptors, Cannabinoid
Reverse Transcriptase Polymerase Chain Reaction
Subcutaneous Fat

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1111/j.1365-2362.2009.02231.x

Cite this

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title = "Reduced cannabinoid receptor 1 protein in subcutaneous adipose tissue of obese",

abstract = "Background Cannabinoid 1 receptors are identified in various tissues involved in the internal metabolism including adipose tissue and the endocannabinoid system is claimed to be overactive in the obese state. To study the potential involvement of cannabinoid receptor 1 in the endocannabinoid system over-activity in adipose tissue in the obese state, we investigated the cannabinoid receptor 1 levels in adipose tissue from different fat depots in lean and obese humans. Materials and methods The adipose tissue samples were analysed by Western blot and by RT-PCR. Results Both the gene expression and the protein of cannabinoid receptor 1 were lower in subcutaneous abdominal adipose tissue from obese subjects as compared with lean subjects (P < 0·01 and P = 0·058). Moreover, in lean subjects, the level of cannabinoid receptor 1 was significantly higher in subcutaneous adipose tissue compared with visceral adipose tissue (P < 0·05) for both gene expression and protein. The level of cannabinoid receptor 1 was similar between the two depots in obese subjects. The expression of cannabinoid receptor 1 was higher in subcutaneous gluteal adipose tissue as compared with subcutaneous abdominal adipose tissue (P < 0·05). Conclusion We found in lean subjects, a robust lower level of cannabinoid receptor 1 in visceral adipose tissue compared with subcutaneous adipose tissue (both RNA and protein levels), but similar levels of cannabinoid receptor 1 between the two depots in obese subjects. Our present findings do not indicate that cannabinoid receptor 1 is directly involved in the endocannabinoid system over-activity in adipose tissue in obesity.",

keywords = "Adipocytes, Adult, Blotting, Western, Female, Humans, Intra-Abdominal Fat, Male, Middle Aged, Obesity, RNA, Messenger, Receptors, Cannabinoid, Reverse Transcriptase Polymerase Chain Reaction, Subcutaneous Fat",

author = "Bennetzen, {Marianne Faurholt} and Nielsen, {Thomas Svava} and Paulsen, {S K} and J Bendix and S Fisker and N Jessen and S Lund and Bj{\o}rn Richelsen and Pedersen, {S B}",

year = "2010",

month = feb,

doi = "10.1111/j.1365-2362.2009.02231.x",

language = "English",

volume = "40",

pages = "121--6",

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TY - JOUR

T1 - Reduced cannabinoid receptor 1 protein in subcutaneous adipose tissue of obese

AU - Bennetzen, Marianne Faurholt

AU - Nielsen, Thomas Svava

AU - Paulsen, S K

AU - Bendix, J

AU - Fisker, S

AU - Jessen, N

AU - Lund, S

AU - Richelsen, Bjørn

AU - Pedersen, S B

PY - 2010/2

Y1 - 2010/2

N2 - Background Cannabinoid 1 receptors are identified in various tissues involved in the internal metabolism including adipose tissue and the endocannabinoid system is claimed to be overactive in the obese state. To study the potential involvement of cannabinoid receptor 1 in the endocannabinoid system over-activity in adipose tissue in the obese state, we investigated the cannabinoid receptor 1 levels in adipose tissue from different fat depots in lean and obese humans. Materials and methods The adipose tissue samples were analysed by Western blot and by RT-PCR. Results Both the gene expression and the protein of cannabinoid receptor 1 were lower in subcutaneous abdominal adipose tissue from obese subjects as compared with lean subjects (P < 0·01 and P = 0·058). Moreover, in lean subjects, the level of cannabinoid receptor 1 was significantly higher in subcutaneous adipose tissue compared with visceral adipose tissue (P < 0·05) for both gene expression and protein. The level of cannabinoid receptor 1 was similar between the two depots in obese subjects. The expression of cannabinoid receptor 1 was higher in subcutaneous gluteal adipose tissue as compared with subcutaneous abdominal adipose tissue (P < 0·05). Conclusion We found in lean subjects, a robust lower level of cannabinoid receptor 1 in visceral adipose tissue compared with subcutaneous adipose tissue (both RNA and protein levels), but similar levels of cannabinoid receptor 1 between the two depots in obese subjects. Our present findings do not indicate that cannabinoid receptor 1 is directly involved in the endocannabinoid system over-activity in adipose tissue in obesity.

AB - Background Cannabinoid 1 receptors are identified in various tissues involved in the internal metabolism including adipose tissue and the endocannabinoid system is claimed to be overactive in the obese state. To study the potential involvement of cannabinoid receptor 1 in the endocannabinoid system over-activity in adipose tissue in the obese state, we investigated the cannabinoid receptor 1 levels in adipose tissue from different fat depots in lean and obese humans. Materials and methods The adipose tissue samples were analysed by Western blot and by RT-PCR. Results Both the gene expression and the protein of cannabinoid receptor 1 were lower in subcutaneous abdominal adipose tissue from obese subjects as compared with lean subjects (P < 0·01 and P = 0·058). Moreover, in lean subjects, the level of cannabinoid receptor 1 was significantly higher in subcutaneous adipose tissue compared with visceral adipose tissue (P < 0·05) for both gene expression and protein. The level of cannabinoid receptor 1 was similar between the two depots in obese subjects. The expression of cannabinoid receptor 1 was higher in subcutaneous gluteal adipose tissue as compared with subcutaneous abdominal adipose tissue (P < 0·05). Conclusion We found in lean subjects, a robust lower level of cannabinoid receptor 1 in visceral adipose tissue compared with subcutaneous adipose tissue (both RNA and protein levels), but similar levels of cannabinoid receptor 1 between the two depots in obese subjects. Our present findings do not indicate that cannabinoid receptor 1 is directly involved in the endocannabinoid system over-activity in adipose tissue in obesity.

KW - Adipocytes

KW - Adult

KW - Blotting, Western

KW - Female

KW - Humans

KW - Intra-Abdominal Fat

KW - Male

KW - Middle Aged

KW - Obesity

KW - RNA, Messenger

KW - Receptors, Cannabinoid

KW - Reverse Transcriptase Polymerase Chain Reaction

KW - Subcutaneous Fat

U2 - 10.1111/j.1365-2362.2009.02231.x

DO - 10.1111/j.1365-2362.2009.02231.x

M3 - Journal article

C2 - 20039929

SN - 0014-2972

VL - 40

SP - 121

EP - 126

JO - Zeitschrift fur klinische Medizin

JF - Zeitschrift fur klinische Medizin

IS - 2

ER -

Reduced cannabinoid receptor 1 protein in subcutaneous adipose tissue of obese

Abstract

Keywords

UN SDGs

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