Redistribution and persistence of microorganisms and steroid hormones after soil-injection of swine slurry

TY - JOUR

T1 - Redistribution and persistence of microorganisms and steroid hormones after soil-injection of swine slurry

AU - Amin, Mostofa

AU - Bech, Tina B.

AU - Forslund, Anita

AU - Hansen, Martin

AU - Petersen, Søren O.

AU - Lægdsmand, Mette

N1 - © 2013.

PY - 2014/1/1

Y1 - 2014/1/1

N2 - The redistribution and fate of contaminants in pig slurry after direct injection were investigated at two field sites, Silstrup (sandy clay loam) and Estrup (sandy loam), in Denmark. Intact soil samples were collected for up to seven weeks after slurry injection and concentrations of Salmonella Typhimurium Bacteriophage 28B (phage 28B), Escherichia coli, steroid hormones and other slurry components (water, volatile solids, chloride and mineral N) determined in and around the injection slit. The two experiments at Silstrup and Estrup differed with respect to slurry solid content (6.3 vs. 0.8%), as well as soil clay content (27 vs. 15%) and differed considerably with respect to the initial redistribution of slurry-borne contaminants in soil. The transport of microorganisms from the slurry injection slit to the surrounding soil was much lower than that of mineral N and chloride due to attachment and entrapment. The redistribution of E. coli was more affected by site-specific conditions compared to phage 28B, possibly due to the larger cell size of E. coli. The overall recovery of phage 28B was 0.8-4%, and of E. coli 0.0-1.3% in different samples, by the end of the study. Nine different steroid hormones were detected in the slurry slit, and a slow redistribution to the surrounding soil was observed. Overall recovery of estrogens was 0.0 to 6.6% in different samples. The study showed that the combination of soil and slurry properties determined the initial spreading of contaminants, and hence the potential for subsequent leaching.

AB - The redistribution and fate of contaminants in pig slurry after direct injection were investigated at two field sites, Silstrup (sandy clay loam) and Estrup (sandy loam), in Denmark. Intact soil samples were collected for up to seven weeks after slurry injection and concentrations of Salmonella Typhimurium Bacteriophage 28B (phage 28B), Escherichia coli, steroid hormones and other slurry components (water, volatile solids, chloride and mineral N) determined in and around the injection slit. The two experiments at Silstrup and Estrup differed with respect to slurry solid content (6.3 vs. 0.8%), as well as soil clay content (27 vs. 15%) and differed considerably with respect to the initial redistribution of slurry-borne contaminants in soil. The transport of microorganisms from the slurry injection slit to the surrounding soil was much lower than that of mineral N and chloride due to attachment and entrapment. The redistribution of E. coli was more affected by site-specific conditions compared to phage 28B, possibly due to the larger cell size of E. coli. The overall recovery of phage 28B was 0.8-4%, and of E. coli 0.0-1.3% in different samples, by the end of the study. Nine different steroid hormones were detected in the slurry slit, and a slow redistribution to the surrounding soil was observed. Overall recovery of estrogens was 0.0 to 6.6% in different samples. The study showed that the combination of soil and slurry properties determined the initial spreading of contaminants, and hence the potential for subsequent leaching.

KW - Animals

KW - Colony Count, Microbial

KW - Denmark

KW - Escherichia coli

KW - Gas Chromatography-Mass Spectrometry

KW - Gonadal Steroid Hormones

KW - Manure

KW - Nitrogen

KW - Salmonella Phages

KW - Soil Microbiology

KW - Soil Pollutants

KW - Sus scrofa

KW - Tandem Mass Spectrometry

KW - water treatment

KW - aecal contaminati on

KW - heavy metals

KW - food hygiene

KW - waste- water nutrients value

U2 - 10.1016/j.scitotenv.2013.07.051

DO - 10.1016/j.scitotenv.2013.07.051

M3 - Journal article

C2 - 23994734

SN - 0048-9697

VL - 466-467

SP - 1003

EP - 1010

JO - Science of the Total Environment

JF - Science of the Total Environment

ER -