Recovery, enrichment and selectivity in liquid-phase microextraction: Comparison with conventional liquid-liquid extraction

TY - JOUR

T1 - Recovery, enrichment and selectivity in liquid-phase microextraction

T2 - Comparison with conventional liquid-liquid extraction

AU - Ho, Tung Si

AU - Pedersen-Bjergaard, Stig

AU - Rasmussen, Knut Einar

PY - 2002/7/19

Y1 - 2002/7/19

N2 - Mathematical descriptions for extraction recovery and enrichment were applied for liquid-phase microextraction (LPME) and comparison with conventional two- and three-phase liquid-liquid extraction techniques (LLE) was made. The LPME theoretical calculations were verified by experimental determination of actual partition coefficients and by data obtained with LPME in a robust hollow fibre formate. With hollow fibre LPME operated in the two-phase mode, analytes were extracted from 1 to 4 ml aqueous samples into 25-50 μl of an organic solvent present in the pores and in the lumen of the porous hollow fibres. Compared with conventional two-phase LLE, two-phase LPME provided substantially higher enrichments for compounds with relatively large partition coefficients (Korg/d>500). In contrast, because of the large volume of organic solvent relative to the sample volume, LLE provided high recovery and moderate enrichment even for compounds with relatively low partition coefficients (Korg/d>5). Thus, two-phase LPME may be used for substantially enhanced extraction selectivity and enrichment of relatively hydrophobic analytes as compared with LLE whereas conventional two-phase LLE is superior for more hydrophilic analytes. Similar results were found for three-phase LPME where analytes where extracted from 1 to 4 ml aqueous samples through approximately 20 μl organic solvent immobilized within the pores of the hollow fibre and into 25 μl of an aqueous acceptor solution inside the lumen of the hollow fibre. The fundamental differences of LPME and LLE were further demonstrated with practical experiments on extraction of the basic drugs promethazine, methadone, and haloperidol from human plasma and urine.

AB - Mathematical descriptions for extraction recovery and enrichment were applied for liquid-phase microextraction (LPME) and comparison with conventional two- and three-phase liquid-liquid extraction techniques (LLE) was made. The LPME theoretical calculations were verified by experimental determination of actual partition coefficients and by data obtained with LPME in a robust hollow fibre formate. With hollow fibre LPME operated in the two-phase mode, analytes were extracted from 1 to 4 ml aqueous samples into 25-50 μl of an organic solvent present in the pores and in the lumen of the porous hollow fibres. Compared with conventional two-phase LLE, two-phase LPME provided substantially higher enrichments for compounds with relatively large partition coefficients (Korg/d>500). In contrast, because of the large volume of organic solvent relative to the sample volume, LLE provided high recovery and moderate enrichment even for compounds with relatively low partition coefficients (Korg/d>5). Thus, two-phase LPME may be used for substantially enhanced extraction selectivity and enrichment of relatively hydrophobic analytes as compared with LLE whereas conventional two-phase LLE is superior for more hydrophilic analytes. Similar results were found for three-phase LPME where analytes where extracted from 1 to 4 ml aqueous samples through approximately 20 μl organic solvent immobilized within the pores of the hollow fibre and into 25 μl of an aqueous acceptor solution inside the lumen of the hollow fibre. The fundamental differences of LPME and LLE were further demonstrated with practical experiments on extraction of the basic drugs promethazine, methadone, and haloperidol from human plasma and urine.

KW - Extraction methods

KW - Haloperidol

KW - Hollow fibres

KW - Liquid-liquid extraction

KW - Liquid-phase microextraction

KW - Mathematical modelling

KW - Methadone

KW - Partition coefficients

KW - Promethazine

KW - Selectivity

UR - http://www.scopus.com/inward/record.url?scp=0037135065&partnerID=8YFLogxK

U2 - 10.1016/S0021-9673(02)00215-7

DO - 10.1016/S0021-9673(02)00215-7

M3 - Journal article

C2 - 12187984

AN - SCOPUS:0037135065

SN - 0301-4770

VL - 963

SP - 3

EP - 17

JO - Journal of Chromatography

JF - Journal of Chromatography

IS - 1-2

ER -