Reconstruction of Optical Thickness from Hoffman Modulation Contrast Images

Niels Holm Olsen; Jon Sporring; Mads Nielsen; Christina Hnida; Søren Ziebe

doi:10.1007/3-540-45103-X_70

Reconstruction of Optical Thickness from Hoffman Modulation Contrast Images

Niels Holm Olsen, Jon Sporring, Mads Nielsen, Christina Hnida, Søren Ziebe

Department of Computer Science

2 Citations (Scopus)

Abstract

Hoffman microscopy imaging systems are part of numerous fertility clinics world-wide. We discuss the physics of the Hoffman imaging system from optical thickness to image intensity, implement a simple, yet fast, reconstruction algorithm using Fast Fourier Transformation and discuss the usability of the method on a number of cells from a human embryo. Novelty is identifying the non-linearity of a typical Hoffman imaging system, and the application of Fourier Transformation to reconstruct the optical thickness.

Original language	English
Title of host publication	Image Analysis : 13th Scandinavian Conference, SCIA 2003 Halmstad, Sweden, June 29 – July 2, 2003 Proceedings
Publisher	Springer
Publication date	2003
Pages	445-454
ISBN (Print)	978-3-540-40601-3
DOIs	https://doi.org/10.1007/3-540-45103-X_70
Publication status	Published - 2003
Event	13th Scandinavian Conference in Image Analysis - SCIA - Halmstad, Sweden Duration: 29 Nov 2010 → … Conference number: 13

Conference

Conference	13th Scandinavian Conference in Image Analysis - SCIA
Number	13
Country/Territory	Sweden
City	Halmstad
Period	29/11/2010 → …

Series	Lecture notes in computer science
Volume	2749/2003
ISSN	0302-9743

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10.1007/3-540-45103-X_70

http://www.springerlink.de/content/3umvxyubw02xy8l6/fulltext.pdf

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Olsen, N. H., Sporring, J., Nielsen, M., Hnida, C., & Ziebe, S. (2003). Reconstruction of Optical Thickness from Hoffman Modulation Contrast Images. In Image Analysis: 13th Scandinavian Conference, SCIA 2003 Halmstad, Sweden, June 29 – July 2, 2003 Proceedings (pp. 445-454). Springer. Lecture notes in computer science Vol. 2749/2003 https://doi.org/10.1007/3-540-45103-X_70

Reconstruction of Optical Thickness from Hoffman Modulation Contrast Images. / Olsen, Niels Holm; Sporring, Jon ; Nielsen, Mads et al.

Image Analysis: 13th Scandinavian Conference, SCIA 2003 Halmstad, Sweden, June 29 – July 2, 2003 Proceedings. Springer, 2003. p. 445-454 (Lecture notes in computer science, Vol. 2749/2003).

Research output: Chapter in Book/Report/Conference proceeding › Article in proceedings › Research › peer-review

Olsen, NH, Sporring, J , Nielsen, M, Hnida, C & Ziebe, S 2003, Reconstruction of Optical Thickness from Hoffman Modulation Contrast Images. in Image Analysis: 13th Scandinavian Conference, SCIA 2003 Halmstad, Sweden, June 29 – July 2, 2003 Proceedings. Springer, Lecture notes in computer science, vol. 2749/2003, pp. 445-454, 13th Scandinavian Conference in Image Analysis - SCIA, Halmstad, Sweden, 29/11/2010. https://doi.org/10.1007/3-540-45103-X_70

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abstract = "Hoffman microscopy imaging systems are part of numerous fertility clinics world-wide. We discuss the physics of the Hoffman imaging system from optical thickness to image intensity, implement a simple, yet fast, reconstruction algorithm using Fast Fourier Transformation and discuss the usability of the method on a number of cells from a human embryo. Novelty is identifying the non-linearity of a typical Hoffman imaging system, and the application of Fourier Transformation to reconstruct the optical thickness.",

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AB - Hoffman microscopy imaging systems are part of numerous fertility clinics world-wide. We discuss the physics of the Hoffman imaging system from optical thickness to image intensity, implement a simple, yet fast, reconstruction algorithm using Fast Fourier Transformation and discuss the usability of the method on a number of cells from a human embryo. Novelty is identifying the non-linearity of a typical Hoffman imaging system, and the application of Fourier Transformation to reconstruct the optical thickness.

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Reconstruction of Optical Thickness from Hoffman Modulation Contrast Images

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