Rationally Engineered Tandem Facial Amphiphiles for Improved Membrane Protein Stabilization Efficacy

Manabendra Das; Yang Du; Jonas S. Mortensen; Parameswaran Hariharan; Hyun Sung Lee; Bernadette Byrne; Claus J. Loland; Lan Guan; Brian K. Kobilka; Pil Seok Chae

doi:10.1002/cbic.201800388

Rationally Engineered Tandem Facial Amphiphiles for Improved Membrane Protein Stabilization Efficacy

Manabendra Das, Yang Du, Jonas S. Mortensen, Parameswaran Hariharan, Hyun Sung Lee, Bernadette Byrne, Claus J. Loland, Lan Guan, Brian K. Kobilka, Pil Seok Chae^*

^*Corresponding author for this work

2 Citations (Scopus)

Abstract

A new family of tandem facial glucosides/maltosides (TFGs/TFMs) for membrane protein manipulation was prepared. The best detergent varied depending on the hydrophobic thickness of the target protein, but ether-based TFMs (TFM-C0E, TFM-C3E, and TFM-C5E) were notable for their ability to confer higher membrane protein stability than the previously developed amide-based TFA-1 (P. S. Chae, K. Gotfryd, J. Pacyna, L. J. W. Miercke, S. G. F. Rasmussen, R. A. Robbins, R. R. Rana, C. J. Loland, B. Kobilka, R. Stroud, B. Byrne, U. Gether, S. H. Gellman, J. Am. Chem. Soc. 2010, 132, 16750–16752). Thus, this study not only introduces novel agents with the potential to be used in membrane protein research but also highlights the importance of both the hydrophobic length and linker functionality of the detergent in stabilizing membrane proteins.

Original language	English
Journal	ChemBioChem
Volume	19
Issue number	20
Pages (from-to)	2225-2232
Number of pages	8
ISSN	1439-4227
DOIs	https://doi.org/10.1002/cbic.201800388
Publication status	Published - 2018

Keywords

amphiphiles
detergent design
detergent faciality
protein stabilization
protein structures

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10.1002/cbic.201800388

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@article{434daf203bb144dc9dc064e38ea62704,

title = "Rationally Engineered Tandem Facial Amphiphiles for Improved Membrane Protein Stabilization Efficacy",

abstract = "A new family of tandem facial glucosides/maltosides (TFGs/TFMs) for membrane protein manipulation was prepared. The best detergent varied depending on the hydrophobic thickness of the target protein, but ether-based TFMs (TFM-C0E, TFM-C3E, and TFM-C5E) were notable for their ability to confer higher membrane protein stability than the previously developed amide-based TFA-1 (P. S. Chae, K. Gotfryd, J. Pacyna, L. J. W. Miercke, S. G. F. Rasmussen, R. A. Robbins, R. R. Rana, C. J. Loland, B. Kobilka, R. Stroud, B. Byrne, U. Gether, S. H. Gellman, J. Am. Chem. Soc. 2010, 132, 16750–16752). Thus, this study not only introduces novel agents with the potential to be used in membrane protein research but also highlights the importance of both the hydrophobic length and linker functionality of the detergent in stabilizing membrane proteins.",

keywords = "amphiphiles, detergent design, detergent faciality, protein stabilization, protein structures",

author = "Manabendra Das and Yang Du and Mortensen, {Jonas S.} and Parameswaran Hariharan and Lee, {Hyun Sung} and Bernadette Byrne and Loland, {Claus J.} and Lan Guan and Kobilka, {Brian K.} and Chae, {Pil Seok}",

year = "2018",

doi = "10.1002/cbic.201800388",

language = "English",

volume = "19",

pages = "2225--2232",

journal = "ChemBioChem",

issn = "1439-4227",

publisher = "Wiley - V C H Verlag GmbH & Co. KGaA",

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T1 - Rationally Engineered Tandem Facial Amphiphiles for Improved Membrane Protein Stabilization Efficacy

AU - Das, Manabendra

AU - Du, Yang

AU - Mortensen, Jonas S.

AU - Hariharan, Parameswaran

AU - Lee, Hyun Sung

AU - Byrne, Bernadette

AU - Loland, Claus J.

AU - Guan, Lan

AU - Kobilka, Brian K.

AU - Chae, Pil Seok

PY - 2018

Y1 - 2018

N2 - A new family of tandem facial glucosides/maltosides (TFGs/TFMs) for membrane protein manipulation was prepared. The best detergent varied depending on the hydrophobic thickness of the target protein, but ether-based TFMs (TFM-C0E, TFM-C3E, and TFM-C5E) were notable for their ability to confer higher membrane protein stability than the previously developed amide-based TFA-1 (P. S. Chae, K. Gotfryd, J. Pacyna, L. J. W. Miercke, S. G. F. Rasmussen, R. A. Robbins, R. R. Rana, C. J. Loland, B. Kobilka, R. Stroud, B. Byrne, U. Gether, S. H. Gellman, J. Am. Chem. Soc. 2010, 132, 16750–16752). Thus, this study not only introduces novel agents with the potential to be used in membrane protein research but also highlights the importance of both the hydrophobic length and linker functionality of the detergent in stabilizing membrane proteins.

AB - A new family of tandem facial glucosides/maltosides (TFGs/TFMs) for membrane protein manipulation was prepared. The best detergent varied depending on the hydrophobic thickness of the target protein, but ether-based TFMs (TFM-C0E, TFM-C3E, and TFM-C5E) were notable for their ability to confer higher membrane protein stability than the previously developed amide-based TFA-1 (P. S. Chae, K. Gotfryd, J. Pacyna, L. J. W. Miercke, S. G. F. Rasmussen, R. A. Robbins, R. R. Rana, C. J. Loland, B. Kobilka, R. Stroud, B. Byrne, U. Gether, S. H. Gellman, J. Am. Chem. Soc. 2010, 132, 16750–16752). Thus, this study not only introduces novel agents with the potential to be used in membrane protein research but also highlights the importance of both the hydrophobic length and linker functionality of the detergent in stabilizing membrane proteins.

KW - amphiphiles

KW - detergent design

KW - detergent faciality

KW - protein stabilization

KW - protein structures

U2 - 10.1002/cbic.201800388

DO - 10.1002/cbic.201800388

M3 - Journal article

C2 - 30070754

AN - SCOPUS:85053614521

SN - 1439-4227

VL - 19

SP - 2225

EP - 2232

JO - ChemBioChem

JF - ChemBioChem

IS - 20

ER -

Rationally Engineered Tandem Facial Amphiphiles for Improved Membrane Protein Stabilization Efficacy

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Keywords

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