Rapid photochemical surface patterning of proteins in thiol-ene based microfluidic devices

Josiane P. Lafleur; Radoslaw Kwapiszewski; Thomas G. Jensen; Jörg P. Kutter

Rapid photochemical surface patterning of proteins in thiol-ene based microfluidic devices

Josiane P. Lafleur, Radoslaw Kwapiszewski, Thomas G. Jensen, Jörg P. Kutter^*

^*Corresponding author for this work

Abstract

The ability to immobilize biomolecules at specific locations on the surface of solid supports is central to many biochip applications. This paper reports the rapid one-step photochemical surface patterning of biomolecules in thiol-ene microfluidic chips. Adjusting the stoichiometric ratio of "thiol" and "ene" monomers present in the microfluidic chip bulk material provides a simple and efficient way of tuning the chip's surface chemistry. Here, thiol-ene chips displaying an excess of functional thiol groups at their surfaces are functionalized with biotin and streptavidin in a controlled fashion using photolithography. We also present quantitative data on the number of functional groups available for surface modification on thiol-ene substrates and their stability.

Original language	English
Title of host publication	Proceedings of the 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012
Number of pages	3
Publisher	Chemical and Biological Microsystems Society
Publication date	1 Jan 2012
Pages	1258-1260
ISBN (Print)	9780979806452
Publication status	Published - 1 Jan 2012

Keywords

Biochips
Biomolecule immobilization
Surface functionalization
Thiol-ene polymers

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Lafleur, J. P., Kwapiszewski, R., Jensen, T. G., & Kutter, J. P. (2012). Rapid photochemical surface patterning of proteins in thiol-ene based microfluidic devices. In Proceedings of the 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012 (pp. 1258-1260). Chemical and Biological Microsystems Society. http://www.scopus.com/inward/record.url?scp=84901814641&partnerID=8YFLogxK

Rapid photochemical surface patterning of proteins in thiol-ene based microfluidic devices. / Lafleur, Josiane P.; Kwapiszewski, Radoslaw; Jensen, Thomas G. et al.

Proceedings of the 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012. Chemical and Biological Microsystems Society, 2012. p. 1258-1260.

Research output: Chapter in Book/Report/Conference proceeding › Article in proceedings › Research › peer-review

Lafleur, JP, Kwapiszewski, R, Jensen, TG & Kutter, JP 2012, Rapid photochemical surface patterning of proteins in thiol-ene based microfluidic devices. in Proceedings of the 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012. Chemical and Biological Microsystems Society, pp. 1258-1260. <http://www.scopus.com/inward/record.url?scp=84901814641&partnerID=8YFLogxK>

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N2 - The ability to immobilize biomolecules at specific locations on the surface of solid supports is central to many biochip applications. This paper reports the rapid one-step photochemical surface patterning of biomolecules in thiol-ene microfluidic chips. Adjusting the stoichiometric ratio of "thiol" and "ene" monomers present in the microfluidic chip bulk material provides a simple and efficient way of tuning the chip's surface chemistry. Here, thiol-ene chips displaying an excess of functional thiol groups at their surfaces are functionalized with biotin and streptavidin in a controlled fashion using photolithography. We also present quantitative data on the number of functional groups available for surface modification on thiol-ene substrates and their stability.

AB - The ability to immobilize biomolecules at specific locations on the surface of solid supports is central to many biochip applications. This paper reports the rapid one-step photochemical surface patterning of biomolecules in thiol-ene microfluidic chips. Adjusting the stoichiometric ratio of "thiol" and "ene" monomers present in the microfluidic chip bulk material provides a simple and efficient way of tuning the chip's surface chemistry. Here, thiol-ene chips displaying an excess of functional thiol groups at their surfaces are functionalized with biotin and streptavidin in a controlled fashion using photolithography. We also present quantitative data on the number of functional groups available for surface modification on thiol-ene substrates and their stability.

KW - Biochips

KW - Biomolecule immobilization

KW - Surface functionalization

KW - Thiol-ene polymers

M3 - Article in proceedings

AN - SCOPUS:84901814641

SN - 9780979806452

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EP - 1260

BT - Proceedings of the 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012

PB - Chemical and Biological Microsystems Society

ER -

Rapid photochemical surface patterning of proteins in thiol-ene based microfluidic devices

Abstract

Keywords

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