Radiosynthesis and characterisation of a potent and selective GPR139 agonist radioligand

Sebastiaan Kuhne; Anne Cathrine Nøhr; AleŠ Marek; TomáŠ Elbert; Anders Bue Klein; Hans Bräuner-Osborne; Petrine Wellendorph; Daniel Sejer Pedersen

doi:10.1039/c5ra21326f

Radiosynthesis and characterisation of a potent and selective GPR139 agonist radioligand

Sebastiaan Kuhne, Anne Cathrine Nøhr, AleŠ Marek, TomáŠ Elbert, Anders Bue Klein, Hans Bräuner-Osborne, Petrine Wellendorph, Daniel Sejer Pedersen^*

^*Corresponding author for this work

6 Citations (Scopus)

Abstract

Compound 1 is a selective and potent agonist of the G protein-coupled receptor GPR139 (EC₅₀ = 39 nM). In this study, we describe the synthesis, radiolabelling and in vitro evaluation of [³H]-1 for the characterisation of GPR139 and its spatial expression in the brain using autoradiography. Two different synthesis routes for the radiolabelling of 1 based on a reductive debromination strategy were investigated using deuterium (D₂, g). The route based on reductive debromination of the bromonaphthyl precursor 5 proved superior over arylbromide 4 and was employed for the radiolabelling experiments. Reductive debromination of precursor 5 was accomplished using ³H₂, Pd/C and triethylamine in DMF at ambient temperature to give target molecule [³H]-1 with a specific activity of 19.3 Ci mmol^-1 and a radiochemical purity of ≥95%. By application of autoradiography and binding studies, it was not possible to discriminate [³H]-1 binding to wildtype mice brains from GPR139 knockout mice brains and total binding from non-specific binding in CHO-k1 cells stably expressing human GPR139 receptor. Based on these experiments we conclude that [³H]-1 is not a suitable radioligand for the characterisation of GPR139.

Original language	English
Journal	RSC Advances
Volume	6
Issue number	2
Pages (from-to)	947-952
Number of pages	6
ISSN	2046-2069
DOIs	https://doi.org/10.1039/c5ra21326f
Publication status	Published - 2016

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@article{3e998e9df83d409a9805e675a2a5a299,

title = "Radiosynthesis and characterisation of a potent and selective GPR139 agonist radioligand",

abstract = "Compound 1 is a selective and potent agonist of the G protein-coupled receptor GPR139 (EC50 = 39 nM). In this study, we describe the synthesis, radiolabelling and in vitro evaluation of [3H]-1 for the characterisation of GPR139 and its spatial expression in the brain using autoradiography. Two different synthesis routes for the radiolabelling of 1 based on a reductive debromination strategy were investigated using deuterium (D2, g). The route based on reductive debromination of the bromonaphthyl precursor 5 proved superior over arylbromide 4 and was employed for the radiolabelling experiments. Reductive debromination of precursor 5 was accomplished using 3H2, Pd/C and triethylamine in DMF at ambient temperature to give target molecule [3H]-1 with a specific activity of 19.3 Ci mmol-1 and a radiochemical purity of ≥95%. By application of autoradiography and binding studies, it was not possible to discriminate [3H]-1 binding to wildtype mice brains from GPR139 knockout mice brains and total binding from non-specific binding in CHO-k1 cells stably expressing human GPR139 receptor. Based on these experiments we conclude that [3H]-1 is not a suitable radioligand for the characterisation of GPR139.",

author = "Sebastiaan Kuhne and N{\o}hr, {Anne Cathrine} and Ale{\v S} Marek and Tom{\'a}{\v S} Elbert and Klein, {Anders Bue} and Hans Br{\"a}uner-Osborne and Petrine Wellendorph and Pedersen, {Daniel Sejer}",

year = "2016",

doi = "10.1039/c5ra21326f",

language = "English",

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pages = "947--952",

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T1 - Radiosynthesis and characterisation of a potent and selective GPR139 agonist radioligand

AU - Kuhne, Sebastiaan

AU - Nøhr, Anne Cathrine

AU - Marek, AleŠ

AU - Elbert, TomáŠ

AU - Klein, Anders Bue

AU - Bräuner-Osborne, Hans

AU - Wellendorph, Petrine

AU - Pedersen, Daniel Sejer

PY - 2016

Y1 - 2016

N2 - Compound 1 is a selective and potent agonist of the G protein-coupled receptor GPR139 (EC50 = 39 nM). In this study, we describe the synthesis, radiolabelling and in vitro evaluation of [3H]-1 for the characterisation of GPR139 and its spatial expression in the brain using autoradiography. Two different synthesis routes for the radiolabelling of 1 based on a reductive debromination strategy were investigated using deuterium (D2, g). The route based on reductive debromination of the bromonaphthyl precursor 5 proved superior over arylbromide 4 and was employed for the radiolabelling experiments. Reductive debromination of precursor 5 was accomplished using 3H2, Pd/C and triethylamine in DMF at ambient temperature to give target molecule [3H]-1 with a specific activity of 19.3 Ci mmol-1 and a radiochemical purity of ≥95%. By application of autoradiography and binding studies, it was not possible to discriminate [3H]-1 binding to wildtype mice brains from GPR139 knockout mice brains and total binding from non-specific binding in CHO-k1 cells stably expressing human GPR139 receptor. Based on these experiments we conclude that [3H]-1 is not a suitable radioligand for the characterisation of GPR139.

AB - Compound 1 is a selective and potent agonist of the G protein-coupled receptor GPR139 (EC50 = 39 nM). In this study, we describe the synthesis, radiolabelling and in vitro evaluation of [3H]-1 for the characterisation of GPR139 and its spatial expression in the brain using autoradiography. Two different synthesis routes for the radiolabelling of 1 based on a reductive debromination strategy were investigated using deuterium (D2, g). The route based on reductive debromination of the bromonaphthyl precursor 5 proved superior over arylbromide 4 and was employed for the radiolabelling experiments. Reductive debromination of precursor 5 was accomplished using 3H2, Pd/C and triethylamine in DMF at ambient temperature to give target molecule [3H]-1 with a specific activity of 19.3 Ci mmol-1 and a radiochemical purity of ≥95%. By application of autoradiography and binding studies, it was not possible to discriminate [3H]-1 binding to wildtype mice brains from GPR139 knockout mice brains and total binding from non-specific binding in CHO-k1 cells stably expressing human GPR139 receptor. Based on these experiments we conclude that [3H]-1 is not a suitable radioligand for the characterisation of GPR139.

U2 - 10.1039/c5ra21326f

DO - 10.1039/c5ra21326f

M3 - Journal article

AN - SCOPUS:84953896371

SN - 2046-2069

VL - 6

SP - 947

EP - 952

JO - RSC Advances

JF - RSC Advances

IS - 2

ER -

Radiosynthesis and characterisation of a potent and selective GPR139 agonist radioligand

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