Quantitative immunohistochemistry: standardization and possible application in research and surgical pathology.

TY - JOUR

T1 - Quantitative immunohistochemistry: standardization and possible application in research and surgical pathology.

AU - Fritz, P

AU - Hönes, J

AU - Lutz, D

AU - Multhaupt, Hinke Arnolda B V H

AU - Mischlinski, A

AU - Dörrer, A

AU - Schwarzmann, P

AU - Tuczek, H V

AU - Müller, W

N1 - Keywords: Arginase; Arthritis, Rheumatoid; Breast; Breast Neoplasms; Cell Line, Transformed; Densitometry; Humans; Image Processing, Computer-Assisted; Immunoenzyme Techniques; Immunoglobulins; Immunohistochemistry; Photometry; Receptors, Estrogen; Synovial Membrane

PY - 1989

Y1 - 1989

N2 - We describe three different methods of calibration of quantitative immunohistochemistry, two of them based on self-made dye-agarose or antigen-agarose substrates. Quantification of immunostained objects (immunosignals) can be done by (1) microscope photometry (plug method) or scanning-photometry; (2) and densitometry of a television transformed microscope image. Both approaches are subjected to the different methods of calibration and three examples of application.

AB - We describe three different methods of calibration of quantitative immunohistochemistry, two of them based on self-made dye-agarose or antigen-agarose substrates. Quantification of immunostained objects (immunosignals) can be done by (1) microscope photometry (plug method) or scanning-photometry; (2) and densitometry of a television transformed microscope image. Both approaches are subjected to the different methods of calibration and three examples of application.

M3 - Journal article

C2 - 2505317

SN - 0567-7556

VL - 37

SP - 213

EP - 219

JO - Acta histochemica. Supplementband

JF - Acta histochemica. Supplementband

ER -