Quantification of vitamin D3 and its hydroxylated metabolites in waxy leaf nightshade (Solanum glaucophyllum Desf.), tomato (Solanum lycopersicum L.) and bell pepper (Capsicum annuum L.)

Rie Bak Jäpelt; Daniele Silvestro; Jørn Smedsgaard; Poul Erik Jensen; Jette Jakobsen

doi:10.1016/j.foodchem.2012.11.064

Quantification of vitamin D₃and its hydroxylated metabolites in waxy leaf nightshade (Solanum glaucophyllum Desf.), tomato (Solanum lycopersicum L.) and bell pepper (Capsicum annuum L.)

Rie Bak Jäpelt, Daniele Silvestro, Jørn Smedsgaard, Poul Erik Jensen, Jette Jakobsen

Section for Molecular Plant Biology

14 Citations (Scopus)

Abstract

Changes in vitamin D₃ and its metabolites were investigated following UVB- and heat-treatment in the leaves of Solanum glaucophyllum Desf.; Solanum lycopersicum L. and Capsicum annuum L. The analytical method used was a sensitive and selective liquid chromatography electrospray ionisation tandem mass spectrometry (LC-ESI-MS/MS) method including Diels-Alder derivatisation. Vitamin D₃ and 25-hydroxy vitamin D₃ were found in the leaves of all plants after UVB-treatment. S. glaucophyllum had the highest content, 200 ng vitamin D₃/g dry weight and 31 ng 25-hydroxy vitamin D₃/g dry weight, and was the only plant that also contained 1,25 dihydroxy vitamin D₃ in both free (32 ng/g dry weight) and glycosylated form (17 ng/g dry weight).

Original language	English
Journal	Food Chemistry
Volume	138
Issue number	2-3
Pages (from-to)	1206-1211
Number of pages	6
ISSN	0308-8146
DOIs	https://doi.org/10.1016/j.foodchem.2012.11.064
Publication status	Published - 1 Jun 2013

Access to Document

10.1016/j.foodchem.2012.11.064

Cite this

Quantification of vitamin D₃and its hydroxylated metabolites in waxy leaf nightshade (Solanum glaucophyllum Desf.), tomato (Solanum lycopersicum L.) and bell pepper (Capsicum annuum L.). / Jäpelt, Rie Bak; Silvestro, Daniele; Smedsgaard, Jørn et al.

In: Food Chemistry, Vol. 138, No. 2-3, 01.06.2013, p. 1206-1211.

Research output: Contribution to journal › Journal article › Research › peer-review

@article{83387525dcb4430cab15654d6f39c91b,

title = "Quantification of vitamin D3 and its hydroxylated metabolites in waxy leaf nightshade (Solanum glaucophyllum Desf.), tomato (Solanum lycopersicum L.) and bell pepper (Capsicum annuum L.)",

abstract = "Changes in vitamin D3 and its metabolites were investigated following UVB- and heat-treatment in the leaves of Solanum glaucophyllum Desf.; Solanum lycopersicum L. and Capsicum annuum L. The analytical method used was a sensitive and selective liquid chromatography electrospray ionisation tandem mass spectrometry (LC-ESI-MS/MS) method including Diels-Alder derivatisation. Vitamin D3 and 25-hydroxy vitamin D3 were found in the leaves of all plants after UVB-treatment. S. glaucophyllum had the highest content, 200 ng vitamin D3/g dry weight and 31 ng 25-hydroxy vitamin D3/g dry weight, and was the only plant that also contained 1,25 dihydroxy vitamin D3 in both free (32 ng/g dry weight) and glycosylated form (17 ng/g dry weight).",

author = "J{\"a}pelt, {Rie Bak} and Daniele Silvestro and J{\o}rn Smedsgaard and Jensen, {Poul Erik} and Jette Jakobsen",

year = "2013",

month = jun,

day = "1",

doi = "10.1016/j.foodchem.2012.11.064",

language = "English",

volume = "138",

pages = "1206--1211",

journal = "Food Chemistry",

issn = "0308-8146",

publisher = "Elsevier",

number = "2-3",

}

TY - JOUR

T1 - Quantification of vitamin D3 and its hydroxylated metabolites in waxy leaf nightshade (Solanum glaucophyllum Desf.), tomato (Solanum lycopersicum L.) and bell pepper (Capsicum annuum L.)

AU - Jäpelt, Rie Bak

AU - Silvestro, Daniele

AU - Smedsgaard, Jørn

AU - Jensen, Poul Erik

AU - Jakobsen, Jette

PY - 2013/6/1

Y1 - 2013/6/1

N2 - Changes in vitamin D3 and its metabolites were investigated following UVB- and heat-treatment in the leaves of Solanum glaucophyllum Desf.; Solanum lycopersicum L. and Capsicum annuum L. The analytical method used was a sensitive and selective liquid chromatography electrospray ionisation tandem mass spectrometry (LC-ESI-MS/MS) method including Diels-Alder derivatisation. Vitamin D3 and 25-hydroxy vitamin D3 were found in the leaves of all plants after UVB-treatment. S. glaucophyllum had the highest content, 200 ng vitamin D3/g dry weight and 31 ng 25-hydroxy vitamin D3/g dry weight, and was the only plant that also contained 1,25 dihydroxy vitamin D3 in both free (32 ng/g dry weight) and glycosylated form (17 ng/g dry weight).

AB - Changes in vitamin D3 and its metabolites were investigated following UVB- and heat-treatment in the leaves of Solanum glaucophyllum Desf.; Solanum lycopersicum L. and Capsicum annuum L. The analytical method used was a sensitive and selective liquid chromatography electrospray ionisation tandem mass spectrometry (LC-ESI-MS/MS) method including Diels-Alder derivatisation. Vitamin D3 and 25-hydroxy vitamin D3 were found in the leaves of all plants after UVB-treatment. S. glaucophyllum had the highest content, 200 ng vitamin D3/g dry weight and 31 ng 25-hydroxy vitamin D3/g dry weight, and was the only plant that also contained 1,25 dihydroxy vitamin D3 in both free (32 ng/g dry weight) and glycosylated form (17 ng/g dry weight).

U2 - 10.1016/j.foodchem.2012.11.064

DO - 10.1016/j.foodchem.2012.11.064

M3 - Journal article

C2 - 23411232

SN - 0308-8146

VL - 138

SP - 1206

EP - 1211

JO - Food Chemistry

JF - Food Chemistry

IS - 2-3

ER -

Quantification of vitamin D3 and its hydroxylated metabolites in waxy leaf nightshade (Solanum glaucophyllum Desf.), tomato (Solanum lycopersicum L.) and bell pepper (Capsicum annuum L.)

Abstract

Access to Document

Fingerprint

Cite this

Quantification of vitamin D₃and its hydroxylated metabolites in waxy leaf nightshade (Solanum glaucophyllum Desf.), tomato (Solanum lycopersicum L.) and bell pepper (Capsicum annuum L.)