Quantification of specific E. coli in gut mucosa from Crohn's disease patients

Stina Rikke Jensen; Lisbeth Nielsen Fink; Carsten Struve; Claus Sternberg; Jens Bo Andersen; Jørn Brynskov; Ole Haagen Nielsen; Susanne Brix

doi:10.1016/j.mimet.2011.04.002

Quantification of specific E. coli in gut mucosa from Crohn's disease patients

Stina Rikke Jensen, Lisbeth Nielsen Fink, Carsten Struve, Claus Sternberg, Jens Bo Andersen, Jørn Brynskov, Ole Haagen Nielsen, Susanne Brix

Department of Clinical Medicine

7 Citations (Scopus)

Abstract

We here present a method based on qRT-PCR to quantify E. coli LF82 in intestinal human samples. Two different primer-probe sets were designed to detect LF82, and a third to target total E. coli. The assay showed high robustness and specificity for detection of LF82 in the presence of intestinal tissue.

Original language	English
Journal	Journal of Microbiological Methods
Volume	86
Issue number	1
Pages (from-to)	111-4
Number of pages	4
ISSN	0167-7012
DOIs	https://doi.org/10.1016/j.mimet.2011.04.002
Publication status	Published - Jul 2011

Keywords

Crohn Disease
DNA Primers
Escherichia coli
Humans
Intestinal Mucosa
Polymerase Chain Reaction
Evaluation Studies
Journal Article
Research Support, Non-U.S. Gov't

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10.1016/j.mimet.2011.04.002

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title = "Quantification of specific E. coli in gut mucosa from Crohn's disease patients",

abstract = "We here present a method based on qRT-PCR to quantify E. coli LF82 in intestinal human samples. Two different primer-probe sets were designed to detect LF82, and a third to target total E. coli. The assay showed high robustness and specificity for detection of LF82 in the presence of intestinal tissue.",

keywords = "Crohn Disease, DNA Primers, Escherichia coli, Humans, Intestinal Mucosa, Polymerase Chain Reaction, Evaluation Studies, Journal Article, Research Support, Non-U.S. Gov't",

author = "Jensen, {Stina Rikke} and Fink, {Lisbeth Nielsen} and Carsten Struve and Claus Sternberg and Andersen, {Jens Bo} and J{\o}rn Brynskov and Nielsen, {Ole Haagen} and Susanne Brix",

year = "2011",

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doi = "10.1016/j.mimet.2011.04.002",

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T1 - Quantification of specific E. coli in gut mucosa from Crohn's disease patients

AU - Jensen, Stina Rikke

AU - Fink, Lisbeth Nielsen

AU - Struve, Carsten

AU - Sternberg, Claus

AU - Andersen, Jens Bo

AU - Brynskov, Jørn

AU - Nielsen, Ole Haagen

AU - Brix, Susanne

PY - 2011/7

Y1 - 2011/7

N2 - We here present a method based on qRT-PCR to quantify E. coli LF82 in intestinal human samples. Two different primer-probe sets were designed to detect LF82, and a third to target total E. coli. The assay showed high robustness and specificity for detection of LF82 in the presence of intestinal tissue.

AB - We here present a method based on qRT-PCR to quantify E. coli LF82 in intestinal human samples. Two different primer-probe sets were designed to detect LF82, and a third to target total E. coli. The assay showed high robustness and specificity for detection of LF82 in the presence of intestinal tissue.

KW - Crohn Disease

KW - DNA Primers

KW - Escherichia coli

KW - Humans

KW - Intestinal Mucosa

KW - Polymerase Chain Reaction

KW - Evaluation Studies

KW - Journal Article

KW - Research Support, Non-U.S. Gov't

U2 - 10.1016/j.mimet.2011.04.002

DO - 10.1016/j.mimet.2011.04.002

M3 - Journal article

C2 - 21504765

SN - 0167-7012

VL - 86

SP - 111

EP - 114

JO - Journal of Microbiological Methods

JF - Journal of Microbiological Methods

IS - 1

ER -

Quantification of specific E. coli in gut mucosa from Crohn's disease patients

Abstract

Keywords

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