Quantification of bioavailable chlortetracycline in pig feces using a bacterial whole-cell biosensor

Lars Hestbjerg Hansen; Frank Aarestrup; Søren Johannes Sørensen

doi:10.1016/S0378-1135(02)00029-9

Quantification of bioavailable chlortetracycline in pig feces using a bacterial whole-cell biosensor

Lars Hestbjerg Hansen, Frank Aarestrup, Søren Johannes Sørensen

22 Citations (Scopus)

Abstract

Bacterial whole-cell biosensors were used to measure the concentration of chlortetracycline (CTC) in the feces of pigs. In this study, the Escherichia coli biosensor used has a detection limit of 0.03 mg/kg CTC in pig feces. The tetracycline concentration was correlated with the appearance and maintenance of fecal coliform bacteria resistant to tetracycline. Initially, large quantities of water-extractable CTC were excreted from the pigs and measurable amounts were detected even at 30 days after treatment cessation. This led to a sharp rise in the number of tetracycline resistant coliform bacteria in the feces, to within the same order of magnitude as the total coliform count. The high level of tetracycline resistance was maintained in spite of the declining concentration of tetracycline.

Original language	English
Journal	Veterinary Microbiology
Volume	87
Issue number	1
Pages (from-to)	51-57
ISSN	0378-1135
DOIs	https://doi.org/10.1016/S0378-1135(02)00029-9
Publication status	Published - 2002

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title = "Quantification of bioavailable chlortetracycline in pig feces using a bacterial whole-cell biosensor",

abstract = "Bacterial whole-cell biosensors were used to measure the concentration of chlortetracycline (CTC) in the feces of pigs. In this study, the Escherichia coli biosensor used has a detection limit of 0.03 mg/kg CTC in pig feces. The tetracycline concentration was correlated with the appearance and maintenance of fecal coliform bacteria resistant to tetracycline. Initially, large quantities of water-extractable CTC were excreted from the pigs and measurable amounts were detected even at 30 days after treatment cessation. This led to a sharp rise in the number of tetracycline resistant coliform bacteria in the feces, to within the same order of magnitude as the total coliform count. The high level of tetracycline resistance was maintained in spite of the declining concentration of tetracycline.",

author = "Hansen, {Lars Hestbjerg} and Frank Aarestrup and S{\o}rensen, {S{\o}ren Johannes}",

note = "Author Keywords: Tetracycline; Pig-bacteria; Whole-cell biosensors; Antibiotic resistance; lacZ",

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T1 - Quantification of bioavailable chlortetracycline in pig feces using a bacterial whole-cell biosensor

AU - Hansen, Lars Hestbjerg

AU - Aarestrup, Frank

AU - Sørensen, Søren Johannes

N1 - Author Keywords: Tetracycline; Pig-bacteria; Whole-cell biosensors; Antibiotic resistance; lacZ

PY - 2002

Y1 - 2002

N2 - Bacterial whole-cell biosensors were used to measure the concentration of chlortetracycline (CTC) in the feces of pigs. In this study, the Escherichia coli biosensor used has a detection limit of 0.03 mg/kg CTC in pig feces. The tetracycline concentration was correlated with the appearance and maintenance of fecal coliform bacteria resistant to tetracycline. Initially, large quantities of water-extractable CTC were excreted from the pigs and measurable amounts were detected even at 30 days after treatment cessation. This led to a sharp rise in the number of tetracycline resistant coliform bacteria in the feces, to within the same order of magnitude as the total coliform count. The high level of tetracycline resistance was maintained in spite of the declining concentration of tetracycline.

AB - Bacterial whole-cell biosensors were used to measure the concentration of chlortetracycline (CTC) in the feces of pigs. In this study, the Escherichia coli biosensor used has a detection limit of 0.03 mg/kg CTC in pig feces. The tetracycline concentration was correlated with the appearance and maintenance of fecal coliform bacteria resistant to tetracycline. Initially, large quantities of water-extractable CTC were excreted from the pigs and measurable amounts were detected even at 30 days after treatment cessation. This led to a sharp rise in the number of tetracycline resistant coliform bacteria in the feces, to within the same order of magnitude as the total coliform count. The high level of tetracycline resistance was maintained in spite of the declining concentration of tetracycline.

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DO - 10.1016/S0378-1135(02)00029-9

M3 - Journal article

SN - 0378-1135

VL - 87

SP - 51

EP - 57

JO - Veterinary Microbiology

JF - Veterinary Microbiology

IS - 1

ER -

Quantification of bioavailable chlortetracycline in pig feces using a bacterial whole-cell biosensor

Abstract

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