Quality assessment of a placental perfusion protocol

Line Mathiesen; Tina Mose; Thit Juul Mørck; Jeanette Kolstrup Søgaard Nielsen; Leif Kofoed Nielsen; Lisa Leth Maroun; Morten Hanefeld Dziegiel; Lise Grupe Larsen; Lisbeth E. Knudsen; Line Mathiesen; Tina Mose; Thit Juul Mørck; Jeanette Kolstrup Søgaard Nielsen; Leif Kofoed Nielsen; Lisa Leth Maroun; Morten Hanefeld Dziegiel; Lise Grupe Larsen; Lisbeth Ehlert Knudsen

doi:10.1016/j.reprotox.2010.01.006

Quality assessment of a placental perfusion protocol

Line Mathiesen, Tina Mose, Thit Juul Mørck, Jeanette Kolstrup Søgaard Nielsen, Leif Kofoed Nielsen, Lisa Leth Maroun, Morten Hanefeld Dziegiel, Lise Grupe Larsen, Lisbeth E. Knudsen, Line Mathiesen, Tina Mose, Thit Juul Mørck, Jeanette Kolstrup Søgaard Nielsen, Leif Kofoed Nielsen, Lisa Leth Maroun, Morten Hanefeld Dziegiel, Lise Grupe Larsen, Lisbeth Ehlert Knudsen

77 Citations (Scopus)

Abstract

Validation of in vitro test systems using the modular approach with steps addressing reliability and relevance is an important aim when developing in vitro tests in e.g. reproductive toxicology. The ex vivo human placental perfusion system may be used for such validation, here presenting the placental perfusion model in Copenhagen including control substances. The positive control substance antipyrine shows no difference in transport regardless of perfusion media used or of terms of delivery (n=59, p<0.05). Negative control studies with FITC marked dextran correspond with leakage criteria (<3mlh^-1 from the fetal reservoir) when adding 2 (n=7) and 20mg (n=9) FITC-dextran/100ml fetal perfusion media. Success rate of the Copenhagen placental perfusions is provided in this study, including considerations and quality control parameters. Three checkpoints suggested to determine success rate revealed that 15% of the cannulated placentae received in one year (n=202) were successfully perfused.

Original language	English
Journal	Reproductive Toxicology
Volume	30
Issue number	1
Pages (from-to)	138-46
Number of pages	9
ISSN	0890-6238
DOIs	https://doi.org/10.1016/j.reprotox.2010.01.006
Publication status	Published - 1 Aug 2010

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Mathiesen, L., Mose, T., Mørck, T. J., Nielsen, J. K. S., Nielsen, L. K., Maroun, L. L., Dziegiel, M. H., Larsen, L. G., Knudsen, L. E., Mathiesen, L., Mose, T., Mørck, T. J., Nielsen, J. K. S., Nielsen, L. K., Maroun, L. L., Dziegiel, M. H., Larsen, L. G., & Knudsen, L. E. (2010). Quality assessment of a placental perfusion protocol. Reproductive Toxicology, 30(1), 138-46. https://doi.org/10.1016/j.reprotox.2010.01.006

Mathiesen, L, Mose, T, Mørck, TJ, Nielsen, JKS, Nielsen, LK, Maroun, LL, Dziegiel, MH, Larsen, LG, Knudsen, LE, Mathiesen, L, Mose, T, Mørck, TJ, Nielsen, JKS, Nielsen, LK, Maroun, LL, Dziegiel, MH, Larsen, LG & Knudsen, LE 2010, 'Quality assessment of a placental perfusion protocol', Reproductive Toxicology, vol. 30, no. 1, pp. 138-46. https://doi.org/10.1016/j.reprotox.2010.01.006

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title = "Quality assessment of a placental perfusion protocol",

abstract = "Validation of in vitro test systems using the modular approach with steps addressing reliability and relevance is an important aim when developing in vitro tests in e.g. reproductive toxicology. The ex vivo human placental perfusion system may be used for such validation, here presenting the placental perfusion model in Copenhagen including control substances. The positive control substance antipyrine shows no difference in transport regardless of perfusion media used or of terms of delivery (n=59, p<0.05). Negative control studies with FITC marked dextran correspond with leakage criteria (<3mlh-1 from the fetal reservoir) when adding 2 (n=7) and 20mg (n=9) FITC-dextran/100ml fetal perfusion media. Success rate of the Copenhagen placental perfusions is provided in this study, including considerations and quality control parameters. Three checkpoints suggested to determine success rate revealed that 15% of the cannulated placentae received in one year (n=202) were successfully perfused.",

author = "Line Mathiesen and Tina Mose and M{\o}rck, {Thit Juul} and Nielsen, {Jeanette Kolstrup S{\o}gaard} and Nielsen, {Leif Kofoed} and Maroun, {Lisa Leth} and Dziegiel, {Morten Hanefeld} and Larsen, {Lise Grupe} and Knudsen, {Lisbeth E.} and Line Mathiesen and Tina Mose and M{\o}rck, {Thit Juul} and Nielsen, {Jeanette Kolstrup S{\o}gaard} and Nielsen, {Leif Kofoed} and Maroun, {Lisa Leth} and Dziegiel, {Morten Hanefeld} and Larsen, {Lise Grupe} and Knudsen, {Lisbeth Ehlert}",

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AU - Maroun, Lisa Leth

AU - Dziegiel, Morten Hanefeld

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AU - Mose, Tina

AU - Mørck, Thit Juul

AU - Nielsen, Jeanette Kolstrup Søgaard

AU - Nielsen, Leif Kofoed

AU - Maroun, Lisa Leth

AU - Dziegiel, Morten Hanefeld

AU - Larsen, Lise Grupe

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AB - Validation of in vitro test systems using the modular approach with steps addressing reliability and relevance is an important aim when developing in vitro tests in e.g. reproductive toxicology. The ex vivo human placental perfusion system may be used for such validation, here presenting the placental perfusion model in Copenhagen including control substances. The positive control substance antipyrine shows no difference in transport regardless of perfusion media used or of terms of delivery (n=59, p<0.05). Negative control studies with FITC marked dextran correspond with leakage criteria (<3mlh-1 from the fetal reservoir) when adding 2 (n=7) and 20mg (n=9) FITC-dextran/100ml fetal perfusion media. Success rate of the Copenhagen placental perfusions is provided in this study, including considerations and quality control parameters. Three checkpoints suggested to determine success rate revealed that 15% of the cannulated placentae received in one year (n=202) were successfully perfused.

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DO - 10.1016/j.reprotox.2010.01.006

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C2 - 20096346

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VL - 30

SP - 138

EP - 146

JO - Reproductive Toxicology

JF - Reproductive Toxicology

IS - 1

ER -

Quality assessment of a placental perfusion protocol

Abstract

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