Purification and characterization of bioactive his6-tagged recombinant human tissue inhibitor of metalloproteinases-1 (TIMP-1) protein expressed at high yields in mammalian cells

Lena Vinther Jensen; Ulrik Axel Lademann; Elisabeth Veyhe Andersen; Peter Højrup; Morten Thaysen-Andersen; Berit Olsen Krogh; Birgitte Martine Viuff; Nils Brünner; Jan Stenvang; José Moreira

doi:10.1016/j.pep.2014.06.013

Purification and characterization of bioactive his₆-tagged recombinant human tissue inhibitor of metalloproteinases-1 (TIMP-1) protein expressed at high yields in mammalian cells

Lena Vinther Jensen, Ulrik Axel Lademann, Elisabeth Veyhe Andersen, Peter Højrup, Morten Thaysen-Andersen, Berit Olsen Krogh, Birgitte Martine Viuff, Nils Brünner, Jan Stenvang, José Moreira

5 Citations (Scopus)

Original language	English
Journal	Protein Expression and Purification
Volume	101
Pages (from-to)	157-164
Number of pages	8
ISSN	1046-5928
DOIs	https://doi.org/10.1016/j.pep.2014.06.013
Publication status	Published - Sept 2014

Keywords

Tissue inhibitor of metalloproteinase-1 protein
His tagged
Bioactive
HEK293E expression system

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

Access to Document

10.1016/j.pep.2014.06.013

Cite this

Jensen, L. V., Lademann, U. A., Andersen, E. V., Højrup, P., Thaysen-Andersen, M., Krogh, B. O., Viuff, B. M., Brünner, N., Stenvang, J., & Moreira, J. (2014). Purification and characterization of bioactive his₆-tagged recombinant human tissue inhibitor of metalloproteinases-1 (TIMP-1) protein expressed at high yields in mammalian cells. Protein Expression and Purification, 101, 157-164. https://doi.org/10.1016/j.pep.2014.06.013

Purification and characterization of bioactive his₆-tagged recombinant human tissue inhibitor of metalloproteinases-1 (TIMP-1) protein expressed at high yields in mammalian cells. / Jensen, Lena Vinther; Lademann, Ulrik Axel; Andersen, Elisabeth Veyhe et al.
In: Protein Expression and Purification, Vol. 101, 09.2014, p. 157-164.

Research output: Contribution to journal › Journal article › Research › peer-review

Jensen, LV, Lademann, UA, Andersen, EV, Højrup, P, Thaysen-Andersen, M, Krogh, BO, Viuff, BM, Brünner, N , Stenvang, J & Moreira, J 2014, 'Purification and characterization of bioactive his₆-tagged recombinant human tissue inhibitor of metalloproteinases-1 (TIMP-1) protein expressed at high yields in mammalian cells', Protein Expression and Purification, vol. 101, pp. 157-164. https://doi.org/10.1016/j.pep.2014.06.013

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title = "Purification and characterization of bioactive his6-tagged recombinant human tissue inhibitor of metalloproteinases-1 (TIMP-1) protein expressed at high yields in mammalian cells",

keywords = "Tissue inhibitor of metalloproteinase-1 protein, His tagged, Bioactive, HEK293E expression system",

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AU - Jensen, Lena Vinther

AU - Lademann, Ulrik Axel

AU - Andersen, Elisabeth Veyhe

AU - Højrup, Peter

AU - Thaysen-Andersen, Morten

AU - Krogh, Berit Olsen

AU - Viuff, Birgitte Martine

AU - Brünner, Nils

AU - Stenvang, Jan

AU - Moreira, José

PY - 2014/9

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KW - Bioactive

KW - HEK293E expression system

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DO - 10.1016/j.pep.2014.06.013

M3 - Journal article

SN - 1046-5928

VL - 101

SP - 157

EP - 164

JO - Protein Expression and Purification

JF - Protein Expression and Purification