Punica granatum sarcotesta lectin (PgTeL) impairs growth, structure, viability, aggregation, and biofilm formation ability of Staphylococcus aureus clinical isolates

Pollyanna Michelle da Silva; Mara Baldry; Pai Peng; Juliane Nancy de Oliveira Silva; Tatiana Soares; Fábio André Brayner; Luiz Carlos Alves; Ana Paula Sampaio Feitosa; Patrícia Maria Guedes Paiva; Hanne Ingmer; Thiago Henrique Napoleão

doi:10.1016/j.ijbiomac.2018.11.030

Punica granatum sarcotesta lectin (PgTeL) impairs growth, structure, viability, aggregation, and biofilm formation ability of Staphylococcus aureus clinical isolates

Pollyanna Michelle da Silva, Mara Baldry, Pai Peng, Juliane Nancy de Oliveira Silva, Tatiana Soares, Fábio André Brayner, Luiz Carlos Alves, Ana Paula Sampaio Feitosa, Patrícia Maria Guedes Paiva, Hanne Ingmer, Thiago Henrique Napoleão^*

^*Corresponding author for this work

Food Safety and Zoonoses

5 Citations (Scopus)

Abstract

In this work, we evaluated the ability of Punica granatum sarcotesta lectin (PgTeL) to impair the growth and viability of the Staphylococcus aureus clinical isolates 8325–4 (non-resistant) and LAC USA300 (MRSA strain). The effects of this lectin on aggregating, hemolytic activity, biofilm-forming ability, and expression of virulence genes (hla, rnaIII, and spa) were also investigated. PgTeL showed antibacterial activity against 8325–4 and LAC USA300 strains by interfering with both the growth (MIC ₅₀ of 6.25 and 12.5 μg/mL, respectively) and survival (MBC values of 25.0 and 50.0 μg/mL, respectively). Culture growth started only at the ninth (8325–4) and tenth (LAC USA300) hour in the presence of PgTeL at MIC ₅₀ , while growth was detected since the first hour in the control. The lectin caused markedly altered cell morphology in both the strains. Although, at the MIC ₅₀ , PgTeL caused structural alterations, most cells were still viable, while at the MBC it promoted cell injury and death. PgTeL showed anti-aggregation effect and exhibited antibiofilm activity against both the isolates. However, the lectin did not interfere with the hemolytic activity of LAC USA300 and with the expression of hla, rnaIII, and spa genes. In conclusion, PgTeL is a lectin with multiple inhibitory effects on S. aureus clinical isolates.

Original language	English
Journal	International Journal of Biological Macromolecules
Volume	123
Pages (from-to)	600-608
Number of pages	9
ISSN	0141-8130
DOIs	https://doi.org/10.1016/j.ijbiomac.2018.11.030
Publication status	Published - 2019

Keywords

agr system
Antibacterial activity
Fruit lectin
MRSA isolate
Pomegranate

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10.1016/j.ijbiomac.2018.11.030

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da Silva, P. M., Baldry, M., Peng, P., de Oliveira Silva, J. N., Soares, T., Brayner, F. A., Alves, L. C., Feitosa, A. P. S., Paiva, P. M. G., Ingmer, H., & Napoleão, T. H. (2019). Punica granatum sarcotesta lectin (PgTeL) impairs growth, structure, viability, aggregation, and biofilm formation ability of Staphylococcus aureus clinical isolates. International Journal of Biological Macromolecules, 123, 600-608. https://doi.org/10.1016/j.ijbiomac.2018.11.030

Punica granatum sarcotesta lectin (PgTeL) impairs growth, structure, viability, aggregation, and biofilm formation ability of Staphylococcus aureus clinical isolates. / da Silva, Pollyanna Michelle; Baldry, Mara; Peng, Pai et al.
In: International Journal of Biological Macromolecules, Vol. 123, 2019, p. 600-608.

Research output: Contribution to journal › Journal article › Research › peer-review

da Silva, PM, Baldry, M, Peng, P, de Oliveira Silva, JN, Soares, T, Brayner, FA, Alves, LC, Feitosa, APS, Paiva, PMG, Ingmer, H & Napoleão, TH 2019, 'Punica granatum sarcotesta lectin (PgTeL) impairs growth, structure, viability, aggregation, and biofilm formation ability of Staphylococcus aureus clinical isolates', International Journal of Biological Macromolecules, vol. 123, pp. 600-608. https://doi.org/10.1016/j.ijbiomac.2018.11.030

@article{413e416c60a84b29910687c1e2d54d54,

title = "Punica granatum sarcotesta lectin (PgTeL) impairs growth, structure, viability, aggregation, and biofilm formation ability of Staphylococcus aureus clinical isolates",

abstract = " In this work, we evaluated the ability of Punica granatum sarcotesta lectin (PgTeL) to impair the growth and viability of the Staphylococcus aureus clinical isolates 8325–4 (non-resistant) and LAC USA300 (MRSA strain). The effects of this lectin on aggregating, hemolytic activity, biofilm-forming ability, and expression of virulence genes (hla, rnaIII, and spa) were also investigated. PgTeL showed antibacterial activity against 8325–4 and LAC USA300 strains by interfering with both the growth (MIC 50 of 6.25 and 12.5 μg/mL, respectively) and survival (MBC values of 25.0 and 50.0 μg/mL, respectively). Culture growth started only at the ninth (8325–4) and tenth (LAC USA300) hour in the presence of PgTeL at MIC 50 , while growth was detected since the first hour in the control. The lectin caused markedly altered cell morphology in both the strains. Although, at the MIC 50 , PgTeL caused structural alterations, most cells were still viable, while at the MBC it promoted cell injury and death. PgTeL showed anti-aggregation effect and exhibited antibiofilm activity against both the isolates. However, the lectin did not interfere with the hemolytic activity of LAC USA300 and with the expression of hla, rnaIII, and spa genes. In conclusion, PgTeL is a lectin with multiple inhibitory effects on S. aureus clinical isolates. ",

keywords = "agr system, Antibacterial activity, Fruit lectin, MRSA isolate, Pomegranate",

author = "{da Silva}, {Pollyanna Michelle} and Mara Baldry and Pai Peng and {de Oliveira Silva}, {Juliane Nancy} and Tatiana Soares and Brayner, {F{\'a}bio Andr{\'e}} and Alves, {Luiz Carlos} and Feitosa, {Ana Paula Sampaio} and Paiva, {Patr{\'i}cia Maria Guedes} and Hanne Ingmer and Napole{\~a}o, {Thiago Henrique}",

year = "2019",

doi = "10.1016/j.ijbiomac.2018.11.030",

language = "English",

volume = "123",

pages = "600--608",

journal = "International Journal of Biological Macromolecules",

issn = "0141-8130",

publisher = "Elsevier",

}

TY - JOUR

T1 - Punica granatum sarcotesta lectin (PgTeL) impairs growth, structure, viability, aggregation, and biofilm formation ability of Staphylococcus aureus clinical isolates

AU - da Silva, Pollyanna Michelle

AU - Baldry, Mara

AU - Peng, Pai

AU - de Oliveira Silva, Juliane Nancy

AU - Soares, Tatiana

AU - Brayner, Fábio André

AU - Alves, Luiz Carlos

AU - Feitosa, Ana Paula Sampaio

AU - Paiva, Patrícia Maria Guedes

AU - Ingmer, Hanne

AU - Napoleão, Thiago Henrique

PY - 2019

Y1 - 2019

N2 - In this work, we evaluated the ability of Punica granatum sarcotesta lectin (PgTeL) to impair the growth and viability of the Staphylococcus aureus clinical isolates 8325–4 (non-resistant) and LAC USA300 (MRSA strain). The effects of this lectin on aggregating, hemolytic activity, biofilm-forming ability, and expression of virulence genes (hla, rnaIII, and spa) were also investigated. PgTeL showed antibacterial activity against 8325–4 and LAC USA300 strains by interfering with both the growth (MIC 50 of 6.25 and 12.5 μg/mL, respectively) and survival (MBC values of 25.0 and 50.0 μg/mL, respectively). Culture growth started only at the ninth (8325–4) and tenth (LAC USA300) hour in the presence of PgTeL at MIC 50 , while growth was detected since the first hour in the control. The lectin caused markedly altered cell morphology in both the strains. Although, at the MIC 50 , PgTeL caused structural alterations, most cells were still viable, while at the MBC it promoted cell injury and death. PgTeL showed anti-aggregation effect and exhibited antibiofilm activity against both the isolates. However, the lectin did not interfere with the hemolytic activity of LAC USA300 and with the expression of hla, rnaIII, and spa genes. In conclusion, PgTeL is a lectin with multiple inhibitory effects on S. aureus clinical isolates.

AB - In this work, we evaluated the ability of Punica granatum sarcotesta lectin (PgTeL) to impair the growth and viability of the Staphylococcus aureus clinical isolates 8325–4 (non-resistant) and LAC USA300 (MRSA strain). The effects of this lectin on aggregating, hemolytic activity, biofilm-forming ability, and expression of virulence genes (hla, rnaIII, and spa) were also investigated. PgTeL showed antibacterial activity against 8325–4 and LAC USA300 strains by interfering with both the growth (MIC 50 of 6.25 and 12.5 μg/mL, respectively) and survival (MBC values of 25.0 and 50.0 μg/mL, respectively). Culture growth started only at the ninth (8325–4) and tenth (LAC USA300) hour in the presence of PgTeL at MIC 50 , while growth was detected since the first hour in the control. The lectin caused markedly altered cell morphology in both the strains. Although, at the MIC 50 , PgTeL caused structural alterations, most cells were still viable, while at the MBC it promoted cell injury and death. PgTeL showed anti-aggregation effect and exhibited antibiofilm activity against both the isolates. However, the lectin did not interfere with the hemolytic activity of LAC USA300 and with the expression of hla, rnaIII, and spa genes. In conclusion, PgTeL is a lectin with multiple inhibitory effects on S. aureus clinical isolates.

KW - agr system

KW - Antibacterial activity

KW - Fruit lectin

KW - MRSA isolate

KW - Pomegranate

U2 - 10.1016/j.ijbiomac.2018.11.030

DO - 10.1016/j.ijbiomac.2018.11.030

M3 - Journal article

C2 - 30414418

AN - SCOPUS:85056659281

SN - 0141-8130

VL - 123

SP - 600

EP - 608

JO - International Journal of Biological Macromolecules

JF - International Journal of Biological Macromolecules

ER -

Punica granatum sarcotesta lectin (PgTeL) impairs growth, structure, viability, aggregation, and biofilm formation ability of Staphylococcus aureus clinical isolates

Abstract

Keywords

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