Pulmonary effects of nanofibrillated celluloses in mice suggest that carboxylation lowers the inflammatory and acute phase responses

Niels Hadrup; Kristina Bram Knudsen; Trine Berthing; Henrik Wolff; Stefan Bengtson; Christian Kofoed; Roall Espersen; Casper Højgaard; Jakob Rahr Winther; Martin Willemoës; Irene Wedin; Markus Nuopponen; Harri Alenius; Hannu Norppa; Håkan Wallin; Ulla Vogel

doi:10.1016/j.etap.2019.01.003

Pulmonary effects of nanofibrillated celluloses in mice suggest that carboxylation lowers the inflammatory and acute phase responses

Niels Hadrup, Kristina Bram Knudsen, Trine Berthing, Henrik Wolff, Stefan Bengtson, Christian Kofoed, Roall Espersen, Casper Højgaard, Jakob Rahr Winther, Martin Willemoës, Irene Wedin, Markus Nuopponen, Harri Alenius, Hannu Norppa, Håkan Wallin, Ulla Vogel^*

^*Corresponding author for this work

Biomolecular Sciences

17 Citations (Scopus)

Abstract

We studied if the pulmonary and systemic toxicity of nanofibrillated celluloses can be reduced by carboxylation. Nanofibrillated celluloses administered at 6 or 18 μg to mice by intratracheal instillation were: 1) FINE NFC, 2–20 μm in length, 2–15 nm in width, 2) AS (−COOH), carboxylated, 0.5–10 μm in length, 4–10 nm in width, containing the biocide BIM MC4901 and 3) BIOCID FINE NFC: as (1) but containing BIM MC4901. FINE NFC administration increased neutrophil influx in BAL and induced SAA3 in plasma. AS (−COOH) produced lower neutrophil influx and systemic SAA3 levels than FINE NFC. Results obtained with BIOCID FINE NFC suggested that BIM MC4901 biocide did not explain the lowered response. Increased DNA damage levels were observed across materials, doses and time points. In conclusion, carboxylation of nanofibrillated cellulose was associated with reduced pulmonary and systemic toxicity, suggesting involvement of OH groups in the inflammatory and acute phase responses.

Original language	English
Journal	Environmental Toxicology and Pharmacology
Volume	66
Pages (from-to)	116-125
Number of pages	10
ISSN	1382-6689
DOIs	https://doi.org/10.1016/j.etap.2019.01.003
Publication status	Published - 2019

Keywords

Genotoxicity
Nanocellulose
Nanomaterial
Nanoparticle
Neutrophils
Serum amyloid A

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10.1016/j.etap.2019.01.003

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Hadrup, N., Knudsen, K. B., Berthing, T., Wolff, H., Bengtson, S., Kofoed, C., Espersen, R., Højgaard, C., Winther, J. R., Willemoës, M., Wedin, I., Nuopponen, M., Alenius, H., Norppa, H., Wallin, H., & Vogel, U. (2019). Pulmonary effects of nanofibrillated celluloses in mice suggest that carboxylation lowers the inflammatory and acute phase responses. Environmental Toxicology and Pharmacology, 66, 116-125. https://doi.org/10.1016/j.etap.2019.01.003

Hadrup, N, Knudsen, KB, Berthing, T, Wolff, H, Bengtson, S, Kofoed, C, Espersen, R, Højgaard, C, Winther, JR , Willemoës, M, Wedin, I, Nuopponen, M, Alenius, H, Norppa, H, Wallin, H & Vogel, U 2019, 'Pulmonary effects of nanofibrillated celluloses in mice suggest that carboxylation lowers the inflammatory and acute phase responses', Environmental Toxicology and Pharmacology, vol. 66, pp. 116-125. https://doi.org/10.1016/j.etap.2019.01.003

@article{cd564dc66c3442958f509c2364f32a52,

title = "Pulmonary effects of nanofibrillated celluloses in mice suggest that carboxylation lowers the inflammatory and acute phase responses",

abstract = "We studied if the pulmonary and systemic toxicity of nanofibrillated celluloses can be reduced by carboxylation. Nanofibrillated celluloses administered at 6 or 18 μg to mice by intratracheal instillation were: 1) FINE NFC, 2–20 μm in length, 2–15 nm in width, 2) AS (−COOH), carboxylated, 0.5–10 μm in length, 4–10 nm in width, containing the biocide BIM MC4901 and 3) BIOCID FINE NFC: as (1) but containing BIM MC4901. FINE NFC administration increased neutrophil influx in BAL and induced SAA3 in plasma. AS (−COOH) produced lower neutrophil influx and systemic SAA3 levels than FINE NFC. Results obtained with BIOCID FINE NFC suggested that BIM MC4901 biocide did not explain the lowered response. Increased DNA damage levels were observed across materials, doses and time points. In conclusion, carboxylation of nanofibrillated cellulose was associated with reduced pulmonary and systemic toxicity, suggesting involvement of OH groups in the inflammatory and acute phase responses.",

keywords = "Genotoxicity, Nanocellulose, Nanomaterial, Nanoparticle, Neutrophils, Serum amyloid A",

author = "Niels Hadrup and Knudsen, {Kristina Bram} and Trine Berthing and Henrik Wolff and Stefan Bengtson and Christian Kofoed and Roall Espersen and Casper H{\o}jgaard and Winther, {Jakob Rahr} and Martin Willemo{\"e}s and Irene Wedin and Markus Nuopponen and Harri Alenius and Hannu Norppa and H{\aa}kan Wallin and Ulla Vogel",

year = "2019",

doi = "10.1016/j.etap.2019.01.003",

language = "English",

volume = "66",

pages = "116--125",

journal = "Environmental Toxicology and Pharmacology",

issn = "1382-6689",

publisher = "Elsevier",

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TY - JOUR

T1 - Pulmonary effects of nanofibrillated celluloses in mice suggest that carboxylation lowers the inflammatory and acute phase responses

AU - Hadrup, Niels

AU - Knudsen, Kristina Bram

AU - Berthing, Trine

AU - Wolff, Henrik

AU - Bengtson, Stefan

AU - Kofoed, Christian

AU - Espersen, Roall

AU - Højgaard, Casper

AU - Winther, Jakob Rahr

AU - Willemoës, Martin

AU - Wedin, Irene

AU - Nuopponen, Markus

AU - Alenius, Harri

AU - Norppa, Hannu

AU - Wallin, Håkan

AU - Vogel, Ulla

PY - 2019

Y1 - 2019

N2 - We studied if the pulmonary and systemic toxicity of nanofibrillated celluloses can be reduced by carboxylation. Nanofibrillated celluloses administered at 6 or 18 μg to mice by intratracheal instillation were: 1) FINE NFC, 2–20 μm in length, 2–15 nm in width, 2) AS (−COOH), carboxylated, 0.5–10 μm in length, 4–10 nm in width, containing the biocide BIM MC4901 and 3) BIOCID FINE NFC: as (1) but containing BIM MC4901. FINE NFC administration increased neutrophil influx in BAL and induced SAA3 in plasma. AS (−COOH) produced lower neutrophil influx and systemic SAA3 levels than FINE NFC. Results obtained with BIOCID FINE NFC suggested that BIM MC4901 biocide did not explain the lowered response. Increased DNA damage levels were observed across materials, doses and time points. In conclusion, carboxylation of nanofibrillated cellulose was associated with reduced pulmonary and systemic toxicity, suggesting involvement of OH groups in the inflammatory and acute phase responses.

AB - We studied if the pulmonary and systemic toxicity of nanofibrillated celluloses can be reduced by carboxylation. Nanofibrillated celluloses administered at 6 or 18 μg to mice by intratracheal instillation were: 1) FINE NFC, 2–20 μm in length, 2–15 nm in width, 2) AS (−COOH), carboxylated, 0.5–10 μm in length, 4–10 nm in width, containing the biocide BIM MC4901 and 3) BIOCID FINE NFC: as (1) but containing BIM MC4901. FINE NFC administration increased neutrophil influx in BAL and induced SAA3 in plasma. AS (−COOH) produced lower neutrophil influx and systemic SAA3 levels than FINE NFC. Results obtained with BIOCID FINE NFC suggested that BIM MC4901 biocide did not explain the lowered response. Increased DNA damage levels were observed across materials, doses and time points. In conclusion, carboxylation of nanofibrillated cellulose was associated with reduced pulmonary and systemic toxicity, suggesting involvement of OH groups in the inflammatory and acute phase responses.

KW - Genotoxicity

KW - Nanocellulose

KW - Nanomaterial

KW - Nanoparticle

KW - Neutrophils

KW - Serum amyloid A

U2 - 10.1016/j.etap.2019.01.003

DO - 10.1016/j.etap.2019.01.003

M3 - Journal article

C2 - 30665014

AN - SCOPUS:85060087147

SN - 1382-6689

VL - 66

SP - 116

EP - 125

JO - Environmental Toxicology and Pharmacology

JF - Environmental Toxicology and Pharmacology

ER -

Pulmonary effects of nanofibrillated celluloses in mice suggest that carboxylation lowers the inflammatory and acute phase responses

Abstract

Keywords

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