Protracted, relapsing and demyelinating experimental autoimmune encephalomyelitis in DA rats immunized with syngeneic spinal cord and incomplete Freund's adjuvant.

J C Lorentzen; Shohreh Issazadeh-Navikas; M Storch; M I Mustafa; H Lassman; C Linington; L Klareskog; T Olsson

Protracted, relapsing and demyelinating experimental autoimmune encephalomyelitis in DA rats immunized with syngeneic spinal cord and incomplete Freund's adjuvant.

J C Lorentzen, Shohreh Issazadeh-Navikas, M Storch, M I Mustafa, H Lassman, C Linington, L Klareskog, T Olsson

121 Citations (Scopus)

Abstract

Experimental autoimmune encephalomyelitis (EAE) is a model for multiple sclerosis (MS). However, MS is a chronic, relapsing and demyelinating disease, whereas EAE in rats is typically a brief and monophasic disorder showing little demyelination. We demonstrate here that DA rats develop severe, protracted and relapsing EAE (SPR-EAE) after a subcutaneous immunization at the tail base with syngeneic spinal cord and incomplete Freund's adjuvant (IFA). The neurological deficits were accompanied by demyelinating inflammatory lesions in the spinal cord, with infiltrating T lymphocytes and perivascular deposition of immunoglobulins and complement. The induction of SPR-EAE was associated with humoral autoreactivity to myelin oligodendrocyte glycoprotein (MOG) and cellular autoreactivity to the rat myelin basic protein (MBP) peptides 69-87 and 87-101. These two peptides, as well as whole rat MBP, were encephalitogenic. In conclusion, we believe that the presently described demyelinating SPR-EAE represents a useful model for MS.

Original language	English
Journal	Journal of Neuroimmunology
Volume	63
Issue number	2
Pages (from-to)	193-205
Number of pages	12
ISSN	0165-5728
Publication status	Published - 1995

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Protracted, relapsing and demyelinating experimental autoimmune encephalomyelitis in DA rats immunized with syngeneic spinal cord and incomplete Freund's adjuvant. / Lorentzen, J C; Issazadeh-Navikas, Shohreh; Storch, M et al.

In: Journal of Neuroimmunology, Vol. 63, No. 2, 1995, p. 193-205.

Research output: Contribution to journal › Journal article › Research › peer-review

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title = "Protracted, relapsing and demyelinating experimental autoimmune encephalomyelitis in DA rats immunized with syngeneic spinal cord and incomplete Freund's adjuvant.",

abstract = "Experimental autoimmune encephalomyelitis (EAE) is a model for multiple sclerosis (MS). However, MS is a chronic, relapsing and demyelinating disease, whereas EAE in rats is typically a brief and monophasic disorder showing little demyelination. We demonstrate here that DA rats develop severe, protracted and relapsing EAE (SPR-EAE) after a subcutaneous immunization at the tail base with syngeneic spinal cord and incomplete Freund's adjuvant (IFA). The neurological deficits were accompanied by demyelinating inflammatory lesions in the spinal cord, with infiltrating T lymphocytes and perivascular deposition of immunoglobulins and complement. The induction of SPR-EAE was associated with humoral autoreactivity to myelin oligodendrocyte glycoprotein (MOG) and cellular autoreactivity to the rat myelin basic protein (MBP) peptides 69-87 and 87-101. These two peptides, as well as whole rat MBP, were encephalitogenic. In conclusion, we believe that the presently described demyelinating SPR-EAE represents a useful model for MS.",

author = "Lorentzen, {J C} and Shohreh Issazadeh-Navikas and M Storch and Mustafa, {M I} and H Lassman and C Linington and L Klareskog and T Olsson",

note = "Keywords: Amino Acid Sequence; Animals; Autoantibodies; Demyelinating Diseases; Disease Models, Animal; Encephalomyelitis, Autoimmune, Experimental; Freund's Adjuvant; Guinea Pigs; Immunization; Immunohistochemistry; Injections, Subcutaneous; Molecular Sequence Data; Myelin Basic Proteins; Myelin-Associated Glycoprotein; Peptides; Phenotype; Rats; Rats, Inbred Strains; Recurrence; Reproducibility of Results; Spinal Cord; Time Factors",

year = "1995",

language = "English",

volume = "63",

pages = "193--205",

journal = "Journal of Neuroimmunology",

issn = "0165-5728",

publisher = "Elsevier",

number = "2",

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TY - JOUR

T1 - Protracted, relapsing and demyelinating experimental autoimmune encephalomyelitis in DA rats immunized with syngeneic spinal cord and incomplete Freund's adjuvant.

AU - Lorentzen, J C

AU - Issazadeh-Navikas, Shohreh

AU - Storch, M

AU - Mustafa, M I

AU - Lassman, H

AU - Linington, C

AU - Klareskog, L

AU - Olsson, T

N1 - Keywords: Amino Acid Sequence; Animals; Autoantibodies; Demyelinating Diseases; Disease Models, Animal; Encephalomyelitis, Autoimmune, Experimental; Freund's Adjuvant; Guinea Pigs; Immunization; Immunohistochemistry; Injections, Subcutaneous; Molecular Sequence Data; Myelin Basic Proteins; Myelin-Associated Glycoprotein; Peptides; Phenotype; Rats; Rats, Inbred Strains; Recurrence; Reproducibility of Results; Spinal Cord; Time Factors

PY - 1995

Y1 - 1995

N2 - Experimental autoimmune encephalomyelitis (EAE) is a model for multiple sclerosis (MS). However, MS is a chronic, relapsing and demyelinating disease, whereas EAE in rats is typically a brief and monophasic disorder showing little demyelination. We demonstrate here that DA rats develop severe, protracted and relapsing EAE (SPR-EAE) after a subcutaneous immunization at the tail base with syngeneic spinal cord and incomplete Freund's adjuvant (IFA). The neurological deficits were accompanied by demyelinating inflammatory lesions in the spinal cord, with infiltrating T lymphocytes and perivascular deposition of immunoglobulins and complement. The induction of SPR-EAE was associated with humoral autoreactivity to myelin oligodendrocyte glycoprotein (MOG) and cellular autoreactivity to the rat myelin basic protein (MBP) peptides 69-87 and 87-101. These two peptides, as well as whole rat MBP, were encephalitogenic. In conclusion, we believe that the presently described demyelinating SPR-EAE represents a useful model for MS.

AB - Experimental autoimmune encephalomyelitis (EAE) is a model for multiple sclerosis (MS). However, MS is a chronic, relapsing and demyelinating disease, whereas EAE in rats is typically a brief and monophasic disorder showing little demyelination. We demonstrate here that DA rats develop severe, protracted and relapsing EAE (SPR-EAE) after a subcutaneous immunization at the tail base with syngeneic spinal cord and incomplete Freund's adjuvant (IFA). The neurological deficits were accompanied by demyelinating inflammatory lesions in the spinal cord, with infiltrating T lymphocytes and perivascular deposition of immunoglobulins and complement. The induction of SPR-EAE was associated with humoral autoreactivity to myelin oligodendrocyte glycoprotein (MOG) and cellular autoreactivity to the rat myelin basic protein (MBP) peptides 69-87 and 87-101. These two peptides, as well as whole rat MBP, were encephalitogenic. In conclusion, we believe that the presently described demyelinating SPR-EAE represents a useful model for MS.

M3 - Journal article

C2 - 8550817

SN - 0165-5728

VL - 63

SP - 193

EP - 205

JO - Journal of Neuroimmunology

JF - Journal of Neuroimmunology

IS - 2

ER -

Protracted, relapsing and demyelinating experimental autoimmune encephalomyelitis in DA rats immunized with syngeneic spinal cord and incomplete Freund's adjuvant.

Abstract

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