TY - JOUR
T1 - Proteomic profiling of archaeological human bone
AU - Sawafuji, Rikai
AU - Cappellini, Enrico
AU - Nagaoka, Tomohito
AU - Fotakis, Anna Katerina
AU - Jersie-Christensen, Rosa Rakownikow
AU - Olsen, Jesper Velgaard
AU - Hirata, Kazuaki
AU - Ueda, Shintaroh
PY - 2017/6
Y1 - 2017/6
N2 - Ancient protein analysis provides clues to human life and diseases from ancient times. Here, we performed shotgun proteomics of human archeological bones for the first time, using rib bones from the Hitotsubashi site (AD 1657–1683) in Tokyo, called Edo in ancient times. The output data obtained were analysed using Gene Ontology and label-free quantification. We detected leucocyte-derived proteins, possibly originating from the bone marrow of the rib. Particularly prevalent and relatively high expression of eosinophil peroxidase suggests the influence of infectious diseases. This scenario is plausible, considering the overcrowding and unhygienic living conditions of the Edo city described in the historical literature. We also observed age-dependent differences in proteome profiles, particularly for proteins involved in developmental processes. Among them, alpha-2-HS-glycoprotein demonstrated a strong negative correlation with age. These results suggest that analysis of ancient proteins could provide a useful indicator of stress, disease, starvation, obesity and other kinds of physiological and pathological information.
AB - Ancient protein analysis provides clues to human life and diseases from ancient times. Here, we performed shotgun proteomics of human archeological bones for the first time, using rib bones from the Hitotsubashi site (AD 1657–1683) in Tokyo, called Edo in ancient times. The output data obtained were analysed using Gene Ontology and label-free quantification. We detected leucocyte-derived proteins, possibly originating from the bone marrow of the rib. Particularly prevalent and relatively high expression of eosinophil peroxidase suggests the influence of infectious diseases. This scenario is plausible, considering the overcrowding and unhygienic living conditions of the Edo city described in the historical literature. We also observed age-dependent differences in proteome profiles, particularly for proteins involved in developmental processes. Among them, alpha-2-HS-glycoprotein demonstrated a strong negative correlation with age. These results suggest that analysis of ancient proteins could provide a useful indicator of stress, disease, starvation, obesity and other kinds of physiological and pathological information.
KW - Age-related changes
KW - Archaeological bone
KW - Immune system
KW - Mass spectrometry
KW - Proteomics
UR - http://www.scopus.com/inward/record.url?scp=85020463061&partnerID=8YFLogxK
U2 - 10.1098/rsos.161004
DO - 10.1098/rsos.161004
M3 - Journal article
C2 - 28680659
AN - SCOPUS:85020463061
SN - 2054-5703
VL - 4
JO - Royal Society Open Science
JF - Royal Society Open Science
IS - 6
M1 - 161004
ER -
