Proteoglycan isolation and analysis.

A Woods; J R Couchman

doi:10.1002/0471143030.cb1007s07

Proteoglycan isolation and analysis.

Nutritional Immunology

Abstract

Proteoglycans can be difficult molecules to isolate and analyze due to large mass, charge, and tendency to aggregate or form macromolecular complexes. This unit describes detailed methods for purification of matrix, cell surface, and cytoskeleton-linked proteoglycans. Methods for analysis of glycoaminoglycan size and type and of core protein species are described.

Original language	English
Journal	Current Protocols in Cell Biology
Volume	Chapter 10
Pages (from-to)	Unit 10.7
ISSN	1934-2500
DOIs	https://doi.org/10.1002/0471143030.cb1007s07
Publication status	Published - 2001

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title = "Proteoglycan isolation and analysis.",

abstract = "Proteoglycans can be difficult molecules to isolate and analyze due to large mass, charge, and tendency to aggregate or form macromolecular complexes. This unit describes detailed methods for purification of matrix, cell surface, and cytoskeleton-linked proteoglycans. Methods for analysis of glycoaminoglycan size and type and of core protein species are described.",

author = "A Woods and Couchman, {J R}",

note = "Keywords: Animals; Cells, Cultured; Extracellular Matrix; Glycosaminoglycans; Humans; Proteoglycans",

year = "2001",

doi = "10.1002/0471143030.cb1007s07",

language = "English",

volume = "Chapter 10",

pages = "Unit 10.7",

journal = "Current Protocols in Cell Biology",

issn = "1934-2500",

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T1 - Proteoglycan isolation and analysis.

AU - Woods, A

AU - Couchman, J R

N1 - Keywords: Animals; Cells, Cultured; Extracellular Matrix; Glycosaminoglycans; Humans; Proteoglycans

PY - 2001

Y1 - 2001

N2 - Proteoglycans can be difficult molecules to isolate and analyze due to large mass, charge, and tendency to aggregate or form macromolecular complexes. This unit describes detailed methods for purification of matrix, cell surface, and cytoskeleton-linked proteoglycans. Methods for analysis of glycoaminoglycan size and type and of core protein species are described.

AB - Proteoglycans can be difficult molecules to isolate and analyze due to large mass, charge, and tendency to aggregate or form macromolecular complexes. This unit describes detailed methods for purification of matrix, cell surface, and cytoskeleton-linked proteoglycans. Methods for analysis of glycoaminoglycan size and type and of core protein species are described.

U2 - 10.1002/0471143030.cb1007s07

DO - 10.1002/0471143030.cb1007s07

M3 - Journal article

C2 - 18228301

SN - 1934-2500

VL - Chapter 10

SP - Unit 10.7

JO - Current Protocols in Cell Biology

JF - Current Protocols in Cell Biology

ER -

Proteoglycan isolation and analysis.

Abstract

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