Protein-containing nutrient supplementation following strength training enhances the effect on muscle mass, strength, and bone formation in postmenopausal women

Lars Holm; J.L. Olesen; K. Matsumoto; Tatsuya Doi; Masao Mizuno; Thomas Junker Alsted; Abigail Mackey; P Schwarz; Michael Kjær

doi:http://dx.doi.org/10.1152/japplphysiol.00935.2007

Protein-containing nutrient supplementation following strength training enhances the effect on muscle mass, strength, and bone formation in postmenopausal women

Lars Holm, J.L. Olesen, K. Matsumoto, Tatsuya Doi, Masao Mizuno, Thomas Junker Alsted, Abigail Mackey, P Schwarz, Michael Kjær

89 Citations (Scopus)

Abstract

We evaluated the response of various muscle and bone adaptation parameters with 24 wk of strength training in healthy, early postmenopausal women when a nutrient supplement (protein, carbohydrate, calcium, and vitamin D) or a placebo supplement (a minimum of energy) was ingested immediately following each training session. At inclusion, each woman was randomly and double-blindedly assigned to a nutrient group or a placebo (control) group. Muscle hypertrophy was evaluated from biopsies, MRI, and dual-energy X-ray absorptiometry (DEXA) scans, and muscle strength was determined in a dynamometer. Bone mineral density (BMD) was measured using DEXA scans, and bone turnover was determined from serum osteocalcin and collagen type I cross-linked carboxyl terminal peptide. The nutrient group improved concentric and isokinetic (60°/s) muscle strength from 6 to 24 wk by 9 ± 3% (P < 0.01), whereas controls showed no change (1 ± 2%, P > 0.05). Only the nutrient group improved lean body mass (P < 0.05) over the 24 wk. BMD responded similarly at the lumbar spine but changed differently in the two groups at the femoral neck (P < 0.05) [control: 0.943 ± 0.028 to 0.930 ± 0.024 g/mm3 (−1.0 ± 1.4%); nutrient group: 0.953 ± 0.051 to 0.978 ± 0.043 g/mm3 (3.8 ± 3.4%)] when adjusted for age, body mass index, and BMD at inclusion. Bone formation displayed an interaction (P < 0.05), mainly caused by increased osteocalcin at 24 wk in the nutrient group. In conclusion, we report that nutrient supplementation results in superior improvements in muscle mass, muscle strength, femoral neck BMD, and bone formation during 24 wk of strength training. The observed differences following such a short intervention emphasize the significance of postexercise nutrient supply on musculoskeletal maintenance.

Original language	Danish
Journal	Journal of Applied Physiology
Volume	105
Issue number	1
Pages (from-to)	274-281
Number of pages	9
ISSN	8750-7587
DOIs	https://doi.org/10.1152/japplphysiol.00935.2007
Publication status	Published - 7 Jan 2008

Access to Document

http://dx.doi.org/10.1152/japplphysiol.00935.2007

Cite this

Holm, L., Olesen, J. L., Matsumoto, K., Doi, T., Mizuno, M., Alsted, T. J., Mackey, A., Schwarz, P., & Kjær, M. (2008). Protein-containing nutrient supplementation following strength training enhances the effect on muscle mass, strength, and bone formation in postmenopausal women. Journal of Applied Physiology, 105(1), 274-281. https://doi.org/10.1152/japplphysiol.00935.2007

Protein-containing nutrient supplementation following strength training enhances the effect on muscle mass, strength, and bone formation in postmenopausal women. / Holm, Lars; Olesen, J.L.; Matsumoto, K. et al.

In: Journal of Applied Physiology, Vol. 105, No. 1, 07.01.2008, p. 274-281.

Research output: Contribution to journal › Journal article › Research › peer-review

Holm, L, Olesen, JL, Matsumoto, K, Doi, T, Mizuno, M, Alsted, TJ, Mackey, A, Schwarz, P & Kjær, M 2008, 'Protein-containing nutrient supplementation following strength training enhances the effect on muscle mass, strength, and bone formation in postmenopausal women', Journal of Applied Physiology, vol. 105, no. 1, pp. 274-281. https://doi.org/10.1152/japplphysiol.00935.2007

@article{5ffac030ea5f459bac1c289db6366651,

title = "Protein-containing nutrient supplementation following strength training enhances the effect on muscle mass, strength, and bone formation in postmenopausal women",

abstract = "We evaluated the response of various muscle and bone adaptation parameters with 24 wk of strength training in healthy, early postmenopausal women when a nutrient supplement (protein, carbohydrate, calcium, and vitamin D) or a placebo supplement (a minimum of energy) was ingested immediately following each training session. At inclusion, each woman was randomly and double-blindedly assigned to a nutrient group or a placebo (control) group. Muscle hypertrophy was evaluated from biopsies, MRI, and dual-energy X-ray absorptiometry (DEXA) scans, and muscle strength was determined in a dynamometer. Bone mineral density (BMD) was measured using DEXA scans, and bone turnover was determined from serum osteocalcin and collagen type I cross-linked carboxyl terminal peptide. The nutrient group improved concentric and isokinetic (60°/s) muscle strength from 6 to 24 wk by 9 ± 3% (P < 0.01), whereas controls showed no change (1 ± 2%, P > 0.05). Only the nutrient group improved lean body mass (P < 0.05) over the 24 wk. BMD responded similarly at the lumbar spine but changed differently in the two groups at the femoral neck (P < 0.05) [control: 0.943 ± 0.028 to 0.930 ± 0.024 g/mm3 (−1.0 ± 1.4%); nutrient group: 0.953 ± 0.051 to 0.978 ± 0.043 g/mm3 (3.8 ± 3.4%)] when adjusted for age, body mass index, and BMD at inclusion. Bone formation displayed an interaction (P < 0.05), mainly caused by increased osteocalcin at 24 wk in the nutrient group. In conclusion, we report that nutrient supplementation results in superior improvements in muscle mass, muscle strength, femoral neck BMD, and bone formation during 24 wk of strength training. The observed differences following such a short intervention emphasize the significance of postexercise nutrient supply on musculoskeletal maintenance.",

author = "Lars Holm and J.L. Olesen and K. Matsumoto and Tatsuya Doi and Masao Mizuno and Alsted, {Thomas Junker} and Abigail Mackey and P Schwarz and Michael Kj{\ae}r",

year = "2008",

month = jan,

day = "7",

doi = "http://dx.doi.org/10.1152/japplphysiol.00935.2007",

language = "Dansk",

volume = "105",

pages = "274--281",

journal = "Journal of Applied Physiology",

issn = "8750-7587",

publisher = "American Physiological Society",

number = "1",

}

TY - JOUR

T1 - Protein-containing nutrient supplementation following strength training enhances the effect on muscle mass, strength, and bone formation in postmenopausal women

AU - Holm, Lars

AU - Olesen, J.L.

AU - Matsumoto, K.

AU - Doi, Tatsuya

AU - Mizuno, Masao

AU - Alsted, Thomas Junker

AU - Mackey, Abigail

AU - Schwarz, P

AU - Kjær, Michael

PY - 2008/1/7

Y1 - 2008/1/7

N2 - We evaluated the response of various muscle and bone adaptation parameters with 24 wk of strength training in healthy, early postmenopausal women when a nutrient supplement (protein, carbohydrate, calcium, and vitamin D) or a placebo supplement (a minimum of energy) was ingested immediately following each training session. At inclusion, each woman was randomly and double-blindedly assigned to a nutrient group or a placebo (control) group. Muscle hypertrophy was evaluated from biopsies, MRI, and dual-energy X-ray absorptiometry (DEXA) scans, and muscle strength was determined in a dynamometer. Bone mineral density (BMD) was measured using DEXA scans, and bone turnover was determined from serum osteocalcin and collagen type I cross-linked carboxyl terminal peptide. The nutrient group improved concentric and isokinetic (60°/s) muscle strength from 6 to 24 wk by 9 ± 3% (P < 0.01), whereas controls showed no change (1 ± 2%, P > 0.05). Only the nutrient group improved lean body mass (P < 0.05) over the 24 wk. BMD responded similarly at the lumbar spine but changed differently in the two groups at the femoral neck (P < 0.05) [control: 0.943 ± 0.028 to 0.930 ± 0.024 g/mm3 (−1.0 ± 1.4%); nutrient group: 0.953 ± 0.051 to 0.978 ± 0.043 g/mm3 (3.8 ± 3.4%)] when adjusted for age, body mass index, and BMD at inclusion. Bone formation displayed an interaction (P < 0.05), mainly caused by increased osteocalcin at 24 wk in the nutrient group. In conclusion, we report that nutrient supplementation results in superior improvements in muscle mass, muscle strength, femoral neck BMD, and bone formation during 24 wk of strength training. The observed differences following such a short intervention emphasize the significance of postexercise nutrient supply on musculoskeletal maintenance.

AB - We evaluated the response of various muscle and bone adaptation parameters with 24 wk of strength training in healthy, early postmenopausal women when a nutrient supplement (protein, carbohydrate, calcium, and vitamin D) or a placebo supplement (a minimum of energy) was ingested immediately following each training session. At inclusion, each woman was randomly and double-blindedly assigned to a nutrient group or a placebo (control) group. Muscle hypertrophy was evaluated from biopsies, MRI, and dual-energy X-ray absorptiometry (DEXA) scans, and muscle strength was determined in a dynamometer. Bone mineral density (BMD) was measured using DEXA scans, and bone turnover was determined from serum osteocalcin and collagen type I cross-linked carboxyl terminal peptide. The nutrient group improved concentric and isokinetic (60°/s) muscle strength from 6 to 24 wk by 9 ± 3% (P < 0.01), whereas controls showed no change (1 ± 2%, P > 0.05). Only the nutrient group improved lean body mass (P < 0.05) over the 24 wk. BMD responded similarly at the lumbar spine but changed differently in the two groups at the femoral neck (P < 0.05) [control: 0.943 ± 0.028 to 0.930 ± 0.024 g/mm3 (−1.0 ± 1.4%); nutrient group: 0.953 ± 0.051 to 0.978 ± 0.043 g/mm3 (3.8 ± 3.4%)] when adjusted for age, body mass index, and BMD at inclusion. Bone formation displayed an interaction (P < 0.05), mainly caused by increased osteocalcin at 24 wk in the nutrient group. In conclusion, we report that nutrient supplementation results in superior improvements in muscle mass, muscle strength, femoral neck BMD, and bone formation during 24 wk of strength training. The observed differences following such a short intervention emphasize the significance of postexercise nutrient supply on musculoskeletal maintenance.

U2 - http://dx.doi.org/10.1152/japplphysiol.00935.2007

DO - http://dx.doi.org/10.1152/japplphysiol.00935.2007

M3 - Tidsskriftartikel

SN - 8750-7587

VL - 105

SP - 274

EP - 281

JO - Journal of Applied Physiology

JF - Journal of Applied Physiology

IS - 1

ER -