TY - JOUR
T1 - Protease and amylase stability in the presence of chelators used in laundry detergent applications
T2 - correlation between chelator properties and enzyme stability in liquid detergents
AU - Lund, Henrik
AU - Kaasgaard, Svend Gunnar
AU - Skagerlind, Peter
AU - Jørgensen, Lene
AU - Jørgensen, Christian Isak
AU - van de Weert, Marco
PY - 2012/5
Y1 - 2012/5
N2 - Chelators are a common ingredient in most laundry detergents. They have a number of different functions such as reducing water hardness, assisting in keeping particulate soil in suspension and the removal of certain stains, thus complementing the action of the anionic surfactants. Another important group of components in a modern liquid detergent is enzymes, mainly proteases and amylases. As the most commonly used enzymes within the detergent industry are dependent on bound calcium ions to maintain conformational stability and function, the presence of both chelators and enzymes in aliquid detergent presents a challenge. The three commonly used Ca 2+ chelators: citrate, DTPA (diethylene triamine pentaacetic acid) and HEDP (1-hydroxyethane-1,1-diyl)bis(phosphonic acid), were studied with regard to their impact on protease and amylase stability in buffer and in a model liquid detergent. Enzyme stability was characterized by differential scanning calorimetry (DSC) and activity studies, and correlated to the chelator-Ca 2+ interaction properties. The results show that a chela-tor's ability to reduce water hardness and its Ca 2+ affinity are in reality two separate aspects in the context of their use in liquid detergents. In the presence of DTPA, stoichiometric surplus of free Ca 2+ is required to maintain sufficient amy-lase and protease stability. In the presence of the weaker chelators, HEDP and citrate, the total Ca 2+ concentration is more important to protein stability than stoichiometric balancing between chelator and Ca 2+. Thus, for these chelators their total concentration only has a minor impact on the Ca + concentration required to maintain or improve enzyme storage stability. The results underline the importance of Ca + in liquid detergent formulations, and suggest how proper balancing of chelators and Ca 2+ can be used to improve overall enzyme stability.
AB - Chelators are a common ingredient in most laundry detergents. They have a number of different functions such as reducing water hardness, assisting in keeping particulate soil in suspension and the removal of certain stains, thus complementing the action of the anionic surfactants. Another important group of components in a modern liquid detergent is enzymes, mainly proteases and amylases. As the most commonly used enzymes within the detergent industry are dependent on bound calcium ions to maintain conformational stability and function, the presence of both chelators and enzymes in aliquid detergent presents a challenge. The three commonly used Ca 2+ chelators: citrate, DTPA (diethylene triamine pentaacetic acid) and HEDP (1-hydroxyethane-1,1-diyl)bis(phosphonic acid), were studied with regard to their impact on protease and amylase stability in buffer and in a model liquid detergent. Enzyme stability was characterized by differential scanning calorimetry (DSC) and activity studies, and correlated to the chelator-Ca 2+ interaction properties. The results show that a chela-tor's ability to reduce water hardness and its Ca 2+ affinity are in reality two separate aspects in the context of their use in liquid detergents. In the presence of DTPA, stoichiometric surplus of free Ca 2+ is required to maintain sufficient amy-lase and protease stability. In the presence of the weaker chelators, HEDP and citrate, the total Ca 2+ concentration is more important to protein stability than stoichiometric balancing between chelator and Ca 2+. Thus, for these chelators their total concentration only has a minor impact on the Ca + concentration required to maintain or improve enzyme storage stability. The results underline the importance of Ca + in liquid detergent formulations, and suggest how proper balancing of chelators and Ca 2+ can be used to improve overall enzyme stability.
U2 - 10.1007/s11743-011-1318-8
DO - 10.1007/s11743-011-1318-8
M3 - Journal article
SN - 1097-3958
VL - 15
SP - 265
EP - 276
JO - Journal of Surfactants and Detergents
JF - Journal of Surfactants and Detergents
IS - 3
ER -