Prostaglandin synthesis can be inhibited locally by infusion of NSAIDS through microdialysis catheters in human skeletal muscle

TY - JOUR

T1 - Prostaglandin synthesis can be inhibited locally by infusion of NSAIDS through microdialysis catheters in human skeletal muscle

AU - Mikkelsen, Ulla Ramer

AU - Helmark, Ida Carøe

AU - Kjaer, Michael

AU - Langberg, Henning

N1 - Times Cited: 0ArticleEnglishLangberg, HBispebjerg Hosp, Inst Sports Med, Bispebjerg Hosp, DK-2400 Copenhagen, DenmarkCited References Count: 18260POAMER PHYSIOLOGICAL SOC9650 ROCKVILLE PIKE, BETHESDA, MD 20814 USABETHESDA

PY - 2008

Y1 - 2008

N2 - Prostaglandins are known to be involved in the regulation of local blood flow within human skeletal muscles during exercise, and the concentration of prostaglandins increases locally and systemically in response to exercise. The systemic release of prostaglandins can be inhibited by oral intake of nonsteroidal anti-inflammatory drugs (NSAIDs). However, to study the local role of prostaglandins, the formation of prostaglandins within the tissue must be controlled. Microdialysis enables determination of local concentrations of water-soluble substances within the tissue. In the present study, the microdialysis method was used to infuse NSAIDs locally into human skeletal muscles producing a local block of prostaglandin formation. In addition, the graded blockade at various distances from the infusion site within the muscle during rest, exercise and recovery was determined. Microdialysis was performed in thigh muscles (vastus lateralis muscle) in six healthy men. One of the microdialysis catheters was used to block prostaglandin synthesis by infusion of the NSAID indomethacin. Additional catheters were placed 1 and 4 cm away from the infusion and in the contralateral leg (working control). Following 2 h of rest, the subjects performed 200 maximal eccentric contractions with each leg followed by 3 h of rest. The study revealed that infusion of NSAID reduced local prostaglandin E(2) concentration by approximately 30-50% (4 cm away from the infusion) and 85% (1 cm away from the infusion) compared with the contralateral (unblocked) thigh muscle. In conclusion, the present study shows that infusion of NSAIDs into human muscle via microdialysis catheters results in a graded blockade of prostaglandin synthesis.

AB - Prostaglandins are known to be involved in the regulation of local blood flow within human skeletal muscles during exercise, and the concentration of prostaglandins increases locally and systemically in response to exercise. The systemic release of prostaglandins can be inhibited by oral intake of nonsteroidal anti-inflammatory drugs (NSAIDs). However, to study the local role of prostaglandins, the formation of prostaglandins within the tissue must be controlled. Microdialysis enables determination of local concentrations of water-soluble substances within the tissue. In the present study, the microdialysis method was used to infuse NSAIDs locally into human skeletal muscles producing a local block of prostaglandin formation. In addition, the graded blockade at various distances from the infusion site within the muscle during rest, exercise and recovery was determined. Microdialysis was performed in thigh muscles (vastus lateralis muscle) in six healthy men. One of the microdialysis catheters was used to block prostaglandin synthesis by infusion of the NSAID indomethacin. Additional catheters were placed 1 and 4 cm away from the infusion and in the contralateral leg (working control). Following 2 h of rest, the subjects performed 200 maximal eccentric contractions with each leg followed by 3 h of rest. The study revealed that infusion of NSAID reduced local prostaglandin E(2) concentration by approximately 30-50% (4 cm away from the infusion) and 85% (1 cm away from the infusion) compared with the contralateral (unblocked) thigh muscle. In conclusion, the present study shows that infusion of NSAIDs into human muscle via microdialysis catheters results in a graded blockade of prostaglandin synthesis.

KW - Adult

KW - Anti-Inflammatory Agents, Non-Steroidal

KW - Catheters, Indwelling

KW - Cyclooxygenase Inhibitors

KW - Dinoprostone

KW - Down-Regulation

KW - Exercise

KW - Humans

KW - Indomethacin

KW - Infusions, Parenteral

KW - Male

KW - Microdialysis

KW - Muscle Contraction

KW - Quadriceps Muscle

KW - Recovery of Function

KW - Time Factors

U2 - 10.1152/japplphysiol.01016.2007

DO - 10.1152/japplphysiol.01016.2007

M3 - Journal article

C2 - 18079272

SN - 8750-7587

VL - 104

SP - 534

EP - 537

JO - Journal of Applied Physiology

JF - Journal of Applied Physiology

IS - 2

ER -