Production of a heterologous proteinase A by Saccharomyces kluyveri

K Møller; L D Tidemand; Jakob R. Winther; Lisbeth Olsson; Jure Piskur; J Nielsen

Production of a heterologous proteinase A by Saccharomyces kluyveri

K Møller, L D Tidemand, Jakob R. Winther, Lisbeth Olsson, Jure Piskur, J Nielsen

Biomolecular Sciences

5 Citations (Scopus)

Abstract

In order to evaluate the potential of Saccharomyces kluyveri for heterologous protein production, S. kluyveri Y159 was transformed with a S. cerevisiae-based multi-copy plasmid containing the S. cerevisiae PEP4 gene, which encodes proteinase A, under the control of its native promoter. As a reference, S. cerevisiae CEN.PK 113-5D was transformed with the same plasmid and the two strains were characterised in batch cultivations on glucose. The glucose metabolism was found to be less fermentative in S. kluyveri than in S. cerevisiae. The yield of ethanol on glucose was 0.11 g/g in S. kluyveri, compared to a yield of 0.40 g/g in S. cerevisiae. Overexpression of PEP4 led to the secretion of active proteinase A in both S. kluyveri and S. cerevisiae. The yield of active proteinase A during growth on glucose was found to be 3.6-fold higher in S. kluyveri than in the S. cerevisiae reference strain.

Original language	English
Journal	Applied Microbiology and Biotechnology
Volume	57
Issue number	1-2
Pages (from-to)	216-9
Number of pages	4
ISSN	0175-7598
Publication status	Published - 2001

Keywords

Aspartic Acid Endopeptidases
Ethanol
Glucose
Plasmids
Promoter Regions, Genetic
Saccharomyces

Cite this

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title = "Production of a heterologous proteinase A by Saccharomyces kluyveri",

abstract = "In order to evaluate the potential of Saccharomyces kluyveri for heterologous protein production, S. kluyveri Y159 was transformed with a S. cerevisiae-based multi-copy plasmid containing the S. cerevisiae PEP4 gene, which encodes proteinase A, under the control of its native promoter. As a reference, S. cerevisiae CEN.PK 113-5D was transformed with the same plasmid and the two strains were characterised in batch cultivations on glucose. The glucose metabolism was found to be less fermentative in S. kluyveri than in S. cerevisiae. The yield of ethanol on glucose was 0.11 g/g in S. kluyveri, compared to a yield of 0.40 g/g in S. cerevisiae. Overexpression of PEP4 led to the secretion of active proteinase A in both S. kluyveri and S. cerevisiae. The yield of active proteinase A during growth on glucose was found to be 3.6-fold higher in S. kluyveri than in the S. cerevisiae reference strain.",

keywords = "Aspartic Acid Endopeptidases, Ethanol, Glucose, Plasmids, Promoter Regions, Genetic, Saccharomyces",

author = "K M{\o}ller and Tidemand, {L D} and Winther, {Jakob R.} and Lisbeth Olsson and Jure Piskur and J Nielsen",

year = "2001",

language = "English",

volume = "57",

pages = "216--9",

journal = "Applied Microbiology and Biotechnology",

issn = "0175-7598",

publisher = "Springer",

number = "1-2",

}

TY - JOUR

T1 - Production of a heterologous proteinase A by Saccharomyces kluyveri

AU - Møller, K

AU - Tidemand, L D

AU - Winther, Jakob R.

AU - Olsson, Lisbeth

AU - Piskur, Jure

AU - Nielsen, J

PY - 2001

Y1 - 2001

N2 - In order to evaluate the potential of Saccharomyces kluyveri for heterologous protein production, S. kluyveri Y159 was transformed with a S. cerevisiae-based multi-copy plasmid containing the S. cerevisiae PEP4 gene, which encodes proteinase A, under the control of its native promoter. As a reference, S. cerevisiae CEN.PK 113-5D was transformed with the same plasmid and the two strains were characterised in batch cultivations on glucose. The glucose metabolism was found to be less fermentative in S. kluyveri than in S. cerevisiae. The yield of ethanol on glucose was 0.11 g/g in S. kluyveri, compared to a yield of 0.40 g/g in S. cerevisiae. Overexpression of PEP4 led to the secretion of active proteinase A in both S. kluyveri and S. cerevisiae. The yield of active proteinase A during growth on glucose was found to be 3.6-fold higher in S. kluyveri than in the S. cerevisiae reference strain.

AB - In order to evaluate the potential of Saccharomyces kluyveri for heterologous protein production, S. kluyveri Y159 was transformed with a S. cerevisiae-based multi-copy plasmid containing the S. cerevisiae PEP4 gene, which encodes proteinase A, under the control of its native promoter. As a reference, S. cerevisiae CEN.PK 113-5D was transformed with the same plasmid and the two strains were characterised in batch cultivations on glucose. The glucose metabolism was found to be less fermentative in S. kluyveri than in S. cerevisiae. The yield of ethanol on glucose was 0.11 g/g in S. kluyveri, compared to a yield of 0.40 g/g in S. cerevisiae. Overexpression of PEP4 led to the secretion of active proteinase A in both S. kluyveri and S. cerevisiae. The yield of active proteinase A during growth on glucose was found to be 3.6-fold higher in S. kluyveri than in the S. cerevisiae reference strain.

KW - Aspartic Acid Endopeptidases

KW - Ethanol

KW - Glucose

KW - Plasmids

KW - Promoter Regions, Genetic

KW - Saccharomyces

M3 - Journal article

C2 - 11693924

SN - 0175-7598

VL - 57

SP - 216

EP - 219

JO - Applied Microbiology and Biotechnology

JF - Applied Microbiology and Biotechnology

IS - 1-2

ER -

Production of a heterologous proteinase A by Saccharomyces kluyveri

Abstract

Keywords

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