Production of 2nd generation bioethanol from lucerne - Optimization of hydrothermal pretreatment

Sune T. Thomsen; Morten Jensen; Jens E. Schmidt

Production of 2^nd generation bioethanol from lucerne - Optimization of hydrothermal pretreatment

Sune T. Thomsen^*, Morten Jensen, Jens E. Schmidt

^*Corresponding author for this work

12 Citations (Scopus)

Abstract

Lucerne (Medicago sativa) has many qualities associated with sustainable agriculture such as nitrogen fixation and high biomass yield. Therefore, there is interest in whether lucerne is a suitable biomass substrate for bioethanol production, and if hydrothermal pretreatment (HTT) of lucerne improves enzymatic convertibility, providing sufficient enzymatic conversion of carbohydrate to simple sugars for ethanol production. The HTT process was optimised for lucerne hay, and the pretreated biomass was assessed by carbohydrate analysis, inhibitor characterisation of liquid phases, and by simultaneous saccharification and fermentation (SSF) of the whole slurry with Cellubrix enzymes and Saccharomyces cerevisiae yeast. The optimal HTT conditions were 205°C for 5 minutes, resulting in pentose recovery of 81%, and an enzymatic convertibility of glucan to monomeric glucose of 74%, facilitating a conversion of 6.2% w/w of untreated material into bioethanol in SSF, which is equivalent to 1,100 litre ethanol per hectare per year.

Original language	English
Journal	BioResources
Volume	7
Issue number	2
Pages (from-to)	1582-1593
Number of pages	12
ISSN	1930-2126
Publication status	Published - May 2012

Keywords

Alfalfa
Bioethanol
Biomass
Chemical composition
Lucerne
Pretreatment

Access to Document

Production of 2nd generation bioethanol from lucerne - Optimization of hydrothermal pretreatmentFinal published version, 135 KB

Cite this

@article{a76c3f30805c4b67b1d999a72e5979c9,

title = "Production of 2nd generation bioethanol from lucerne - Optimization of hydrothermal pretreatment",

abstract = "Lucerne (Medicago sativa) has many qualities associated with sustainable agriculture such as nitrogen fixation and high biomass yield. Therefore, there is interest in whether lucerne is a suitable biomass substrate for bioethanol production, and if hydrothermal pretreatment (HTT) of lucerne improves enzymatic convertibility, providing sufficient enzymatic conversion of carbohydrate to simple sugars for ethanol production. The HTT process was optimised for lucerne hay, and the pretreated biomass was assessed by carbohydrate analysis, inhibitor characterisation of liquid phases, and by simultaneous saccharification and fermentation (SSF) of the whole slurry with Cellubrix enzymes and Saccharomyces cerevisiae yeast. The optimal HTT conditions were 205°C for 5 minutes, resulting in pentose recovery of 81%, and an enzymatic convertibility of glucan to monomeric glucose of 74%, facilitating a conversion of 6.2% w/w of untreated material into bioethanol in SSF, which is equivalent to 1,100 litre ethanol per hectare per year.",

keywords = "Alfalfa, Bioethanol, Biomass, Chemical composition, Lucerne, Pretreatment",

author = "Thomsen, {Sune T.} and Morten Jensen and Schmidt, {Jens E.}",

year = "2012",

month = may,

language = "English",

volume = "7",

pages = "1582--1593",

journal = "BioResources",

issn = "1930-2126",

publisher = "North Carolina State University",

number = "2",

}

TY - JOUR

T1 - Production of 2nd generation bioethanol from lucerne - Optimization of hydrothermal pretreatment

AU - Thomsen, Sune T.

AU - Jensen, Morten

AU - Schmidt, Jens E.

PY - 2012/5

Y1 - 2012/5

N2 - Lucerne (Medicago sativa) has many qualities associated with sustainable agriculture such as nitrogen fixation and high biomass yield. Therefore, there is interest in whether lucerne is a suitable biomass substrate for bioethanol production, and if hydrothermal pretreatment (HTT) of lucerne improves enzymatic convertibility, providing sufficient enzymatic conversion of carbohydrate to simple sugars for ethanol production. The HTT process was optimised for lucerne hay, and the pretreated biomass was assessed by carbohydrate analysis, inhibitor characterisation of liquid phases, and by simultaneous saccharification and fermentation (SSF) of the whole slurry with Cellubrix enzymes and Saccharomyces cerevisiae yeast. The optimal HTT conditions were 205°C for 5 minutes, resulting in pentose recovery of 81%, and an enzymatic convertibility of glucan to monomeric glucose of 74%, facilitating a conversion of 6.2% w/w of untreated material into bioethanol in SSF, which is equivalent to 1,100 litre ethanol per hectare per year.

AB - Lucerne (Medicago sativa) has many qualities associated with sustainable agriculture such as nitrogen fixation and high biomass yield. Therefore, there is interest in whether lucerne is a suitable biomass substrate for bioethanol production, and if hydrothermal pretreatment (HTT) of lucerne improves enzymatic convertibility, providing sufficient enzymatic conversion of carbohydrate to simple sugars for ethanol production. The HTT process was optimised for lucerne hay, and the pretreated biomass was assessed by carbohydrate analysis, inhibitor characterisation of liquid phases, and by simultaneous saccharification and fermentation (SSF) of the whole slurry with Cellubrix enzymes and Saccharomyces cerevisiae yeast. The optimal HTT conditions were 205°C for 5 minutes, resulting in pentose recovery of 81%, and an enzymatic convertibility of glucan to monomeric glucose of 74%, facilitating a conversion of 6.2% w/w of untreated material into bioethanol in SSF, which is equivalent to 1,100 litre ethanol per hectare per year.

KW - Alfalfa

KW - Bioethanol

KW - Biomass

KW - Chemical composition

KW - Lucerne

KW - Pretreatment

M3 - Journal article

AN - SCOPUS:84863816879

SN - 1930-2126

VL - 7

SP - 1582

EP - 1593

JO - BioResources

JF - BioResources

IS - 2

ER -