Abstract
Homologous recombination repair (HRR) is an evolutionarily conserved cellular process that is important for the maintenance of genome stability during S-phase. Inactivation of the Saccharomyces cerevisiae Sgs1-Top3-Rmi1 complex leads to the accumulation of unprocessed, X-shaped HRR intermediates (X-structures) following replicative stress. Further characterization of these X-structures may reveal why loss of BLM (the human Sgs1 ortholog) leads to the human cancer-predisposition disorder, Bloom's syndrome. In two recent complementary studies, we examined the nature of the X-structures arising in yeast strains lacking Sgs1, Top3 or Rmi1, by identifying which proteins could process these structures in vivo. We revealed that the unprocessed X-structures that accumulate in these strains could be resolved by the ectopic overexpression of two different Holliday junction (HJ) resolvases, and that the endogenous Mus81-Mms4 endonuclease could also remove them, albeit slowly. In this review, we discuss the implications of these results and review the putative roles for the Sgs1-Top3-Rmi1 and Mus81-Mms4 complexes in the processing of various types of HRR intermediates during S phase.
| Original language | English |
|---|---|
| Journal | Cell Cycle |
| Volume | 10 |
| Issue number | 18 |
| Pages (from-to) | 3078-85 |
| Number of pages | 8 |
| ISSN | 1538-4101 |
| Publication status | Published - 15 Sept 2011 |
Keywords
- DNA Breaks, Single-Stranded
- DNA Replication
- DNA, Cruciform
- DNA-Binding Proteins
- Endonucleases
- Holliday Junction Resolvases
- Humans
- RecQ Helicases
- Recombinational DNA Repair
- Saccharomyces cerevisiae
- Saccharomyces cerevisiae Proteins
