Probing glycolytic and membrane potential oscillations in Saccharomyces cerevisiae

Allan K Poulsen; Ann Zahle Andersen; Jens Christian Brasen; Anne Marie Scharff-Poulsen; Lars Folke Olsen

doi:10.1021/bi800396e

Probing glycolytic and membrane potential oscillations in Saccharomyces cerevisiae

Allan K Poulsen, Ann Zahle Andersen, Jens Christian Brasen, Anne Marie Scharff-Poulsen, Lars Folke Olsen

Renal and Vascular Research

24 Citations (Scopus)

Abstract

We have investigated glycolytic oscillations under semi-anaerobic conditions in Saccharomyces cerevisiae by means of NADH fluorescence, measurements of intracellular glucose concentration, and mitochondrial membrane potential. The glucose concentration was measured using an optical nanosensor, while mitochondrial membrane potential was measured using the fluorescent dye DiOC 2(3). The results show that, as opposed to NADH and other intermediates in glycolysis, intracellular glucose is not oscillating. Furthermore, oscillations in NADH and membrane potential are inhibited by the ATP/ADP antiporter inhibitor atractyloside and high concentrations of the ATPase inhibitor N, N'-dicyclohexylcarbodiimide, suggesting that there is a strong coupling between oscillations in mitochondrial membrane potential and oscillations in NADH mediated by the ATP/ADP antiporter and possibly also other respiratory components.

Original language	English
Journal	Biochemistry
Volume	47
Issue number	28
Pages (from-to)	7477-84
Number of pages	8
ISSN	1520-4995
DOIs	https://doi.org/10.1021/bi800396e
Publication status	Published - 15 Jul 2008

Keywords

Aerobiosis
Anaerobiosis
Biosensing Techniques
Electroporation
Fluorescent Dyes
Glucose
Glycolysis
Kinetics
Membrane Potentials
Nanotechnology
Oscillometry
Oxygen Consumption
Saccharomyces cerevisiae

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10.1021/bi800396e

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title = "Probing glycolytic and membrane potential oscillations in Saccharomyces cerevisiae",

abstract = "We have investigated glycolytic oscillations under semi-anaerobic conditions in Saccharomyces cerevisiae by means of NADH fluorescence, measurements of intracellular glucose concentration, and mitochondrial membrane potential. The glucose concentration was measured using an optical nanosensor, while mitochondrial membrane potential was measured using the fluorescent dye DiOC 2(3). The results show that, as opposed to NADH and other intermediates in glycolysis, intracellular glucose is not oscillating. Furthermore, oscillations in NADH and membrane potential are inhibited by the ATP/ADP antiporter inhibitor atractyloside and high concentrations of the ATPase inhibitor N, N'-dicyclohexylcarbodiimide, suggesting that there is a strong coupling between oscillations in mitochondrial membrane potential and oscillations in NADH mediated by the ATP/ADP antiporter and possibly also other respiratory components.",

keywords = "Aerobiosis, Anaerobiosis, Biosensing Techniques, Electroporation, Fluorescent Dyes, Glucose, Glycolysis, Kinetics, Membrane Potentials, Nanotechnology, Oscillometry, Oxygen Consumption, Saccharomyces cerevisiae",

author = "Poulsen, {Allan K} and Andersen, {Ann Zahle} and Brasen, {Jens Christian} and Scharff-Poulsen, {Anne Marie} and Olsen, {Lars Folke}",

year = "2008",

month = jul,

day = "15",

doi = "10.1021/bi800396e",

language = "English",

volume = "47",

pages = "7477--84",

journal = "Biochemistry",

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publisher = "American Chemical Society",

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TY - JOUR

T1 - Probing glycolytic and membrane potential oscillations in Saccharomyces cerevisiae

AU - Poulsen, Allan K

AU - Andersen, Ann Zahle

AU - Brasen, Jens Christian

AU - Scharff-Poulsen, Anne Marie

AU - Olsen, Lars Folke

PY - 2008/7/15

Y1 - 2008/7/15

N2 - We have investigated glycolytic oscillations under semi-anaerobic conditions in Saccharomyces cerevisiae by means of NADH fluorescence, measurements of intracellular glucose concentration, and mitochondrial membrane potential. The glucose concentration was measured using an optical nanosensor, while mitochondrial membrane potential was measured using the fluorescent dye DiOC 2(3). The results show that, as opposed to NADH and other intermediates in glycolysis, intracellular glucose is not oscillating. Furthermore, oscillations in NADH and membrane potential are inhibited by the ATP/ADP antiporter inhibitor atractyloside and high concentrations of the ATPase inhibitor N, N'-dicyclohexylcarbodiimide, suggesting that there is a strong coupling between oscillations in mitochondrial membrane potential and oscillations in NADH mediated by the ATP/ADP antiporter and possibly also other respiratory components.

AB - We have investigated glycolytic oscillations under semi-anaerobic conditions in Saccharomyces cerevisiae by means of NADH fluorescence, measurements of intracellular glucose concentration, and mitochondrial membrane potential. The glucose concentration was measured using an optical nanosensor, while mitochondrial membrane potential was measured using the fluorescent dye DiOC 2(3). The results show that, as opposed to NADH and other intermediates in glycolysis, intracellular glucose is not oscillating. Furthermore, oscillations in NADH and membrane potential are inhibited by the ATP/ADP antiporter inhibitor atractyloside and high concentrations of the ATPase inhibitor N, N'-dicyclohexylcarbodiimide, suggesting that there is a strong coupling between oscillations in mitochondrial membrane potential and oscillations in NADH mediated by the ATP/ADP antiporter and possibly also other respiratory components.

KW - Aerobiosis

KW - Anaerobiosis

KW - Biosensing Techniques

KW - Electroporation

KW - Fluorescent Dyes

KW - Glucose

KW - Glycolysis

KW - Kinetics

KW - Membrane Potentials

KW - Nanotechnology

KW - Oscillometry

KW - Oxygen Consumption

KW - Saccharomyces cerevisiae

U2 - 10.1021/bi800396e

DO - 10.1021/bi800396e

M3 - Journal article

C2 - 18558716

SN - 0006-2960

VL - 47

SP - 7477

EP - 7484

JO - Biochemistry

JF - Biochemistry

IS - 28

ER -

Probing glycolytic and membrane potential oscillations in Saccharomyces cerevisiae

Abstract

Keywords

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