Probing cytochrome c in living mitochondria with surface-enhanced Raman spectroscopy

Nadezda A. Brazhe; Andrey B. Evlyukhin; Eugene A. Goodilin; Anna A. Semenova; Sergey M. Novikov; Sergey I. Bozhevolnyi; Boris N. Chichkov; Asya S. Sarycheva; Adil A. Baizhumanov; Evelina I. Nikelshparg; Leonid I. Deev; Eugene G. Maksimov; Georgy V. Maksimov; Olga Sosnovtseva

doi:10.1038/srep13793

Probing cytochrome c in living mitochondria with surface-enhanced Raman spectroscopy

Nadezda A. Brazhe, Andrey B. Evlyukhin, Eugene A. Goodilin, Anna A. Semenova, Sergey M. Novikov, Sergey I. Bozhevolnyi, Boris N. Chichkov, Asya S. Sarycheva, Adil A. Baizhumanov, Evelina I. Nikelshparg, Leonid I. Deev, Eugene G. Maksimov, Georgy V. Maksimov, Olga Sosnovtseva

49 Citations (Scopus)

Abstract

Selective study of the electron transport chain components in living mitochondria is essential for fundamental biophysical research and for the development of new medical diagnostic methods. However, many important details of inter- and intramembrane mitochondrial processes have remained in shadow due to the lack of non-invasive techniques. Here we suggest a novel label-free approach based on the surface-enhanced Raman spectroscopy (SERS) to monitor the redox state and conformation of cytochrome c in the electron transport chain in living mitochondria. We demonstrate that SERS spectra of living mitochondria placed on hierarchically structured silver-ring substrates provide exclusive information about cytochrome c behavior under modulation of inner mitochondrial membrane potential, proton gradient and the activity of ATP-synthetase. Mathematical simulation explains the observed enhancement of Raman scattering due to high concentration of electric near-field and large contact area between mitochondria and nanostructured surfaces.

Original language	English
Article number	13793
Journal	Scientific Reports
Volume	5
Pages (from-to)	1-13
Number of pages	13
ISSN	2045-2322
DOIs	https://doi.org/10.1038/srep13793
Publication status	Published - 8 Sept 2015

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10.1038/srep13793Licence: CC BY

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Brazhe, N. A., Evlyukhin, A. B., Goodilin, E. A., Semenova, A. A., Novikov, S. M., Bozhevolnyi, S. I., Chichkov, B. N., Sarycheva, A. S., Baizhumanov, A. A., Nikelshparg, E. I., Deev, L. I., Maksimov, E. G., Maksimov, G. V., & Sosnovtseva, O. (2015). Probing cytochrome c in living mitochondria with surface-enhanced Raman spectroscopy. Scientific Reports, 5, 1-13. Article 13793 . https://doi.org/10.1038/srep13793

Brazhe, NA, Evlyukhin, AB, Goodilin, EA, Semenova, AA, Novikov, SM, Bozhevolnyi, SI, Chichkov, BN, Sarycheva, AS, Baizhumanov, AA, Nikelshparg, EI, Deev, LI, Maksimov, EG, Maksimov, GV & Sosnovtseva, O 2015, 'Probing cytochrome c in living mitochondria with surface-enhanced Raman spectroscopy', Scientific Reports, vol. 5, 13793 , pp. 1-13. https://doi.org/10.1038/srep13793

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title = "Probing cytochrome c in living mitochondria with surface-enhanced Raman spectroscopy",

abstract = "Selective study of the electron transport chain components in living mitochondria is essential for fundamental biophysical research and for the development of new medical diagnostic methods. However, many important details of inter- and intramembrane mitochondrial processes have remained in shadow due to the lack of non-invasive techniques. Here we suggest a novel label-free approach based on the surface-enhanced Raman spectroscopy (SERS) to monitor the redox state and conformation of cytochrome c in the electron transport chain in living mitochondria. We demonstrate that SERS spectra of living mitochondria placed on hierarchically structured silver-ring substrates provide exclusive information about cytochrome c behavior under modulation of inner mitochondrial membrane potential, proton gradient and the activity of ATP-synthetase. Mathematical simulation explains the observed enhancement of Raman scattering due to high concentration of electric near-field and large contact area between mitochondria and nanostructured surfaces.",

author = "Brazhe, {Nadezda A.} and Evlyukhin, {Andrey B.} and Goodilin, {Eugene A.} and Semenova, {Anna A.} and Novikov, {Sergey M.} and Bozhevolnyi, {Sergey I.} and Chichkov, {Boris N.} and Sarycheva, {Asya S.} and Baizhumanov, {Adil A.} and Nikelshparg, {Evelina I.} and Deev, {Leonid I.} and Maksimov, {Eugene G.} and Maksimov, {Georgy V.} and Olga Sosnovtseva",

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AU - Brazhe, Nadezda A.

AU - Evlyukhin, Andrey B.

AU - Goodilin, Eugene A.

AU - Semenova, Anna A.

AU - Novikov, Sergey M.

AU - Bozhevolnyi, Sergey I.

AU - Chichkov, Boris N.

AU - Sarycheva, Asya S.

AU - Baizhumanov, Adil A.

AU - Nikelshparg, Evelina I.

AU - Deev, Leonid I.

AU - Maksimov, Eugene G.

AU - Maksimov, Georgy V.

AU - Sosnovtseva, Olga

PY - 2015/9/8

Y1 - 2015/9/8

N2 - Selective study of the electron transport chain components in living mitochondria is essential for fundamental biophysical research and for the development of new medical diagnostic methods. However, many important details of inter- and intramembrane mitochondrial processes have remained in shadow due to the lack of non-invasive techniques. Here we suggest a novel label-free approach based on the surface-enhanced Raman spectroscopy (SERS) to monitor the redox state and conformation of cytochrome c in the electron transport chain in living mitochondria. We demonstrate that SERS spectra of living mitochondria placed on hierarchically structured silver-ring substrates provide exclusive information about cytochrome c behavior under modulation of inner mitochondrial membrane potential, proton gradient and the activity of ATP-synthetase. Mathematical simulation explains the observed enhancement of Raman scattering due to high concentration of electric near-field and large contact area between mitochondria and nanostructured surfaces.

AB - Selective study of the electron transport chain components in living mitochondria is essential for fundamental biophysical research and for the development of new medical diagnostic methods. However, many important details of inter- and intramembrane mitochondrial processes have remained in shadow due to the lack of non-invasive techniques. Here we suggest a novel label-free approach based on the surface-enhanced Raman spectroscopy (SERS) to monitor the redox state and conformation of cytochrome c in the electron transport chain in living mitochondria. We demonstrate that SERS spectra of living mitochondria placed on hierarchically structured silver-ring substrates provide exclusive information about cytochrome c behavior under modulation of inner mitochondrial membrane potential, proton gradient and the activity of ATP-synthetase. Mathematical simulation explains the observed enhancement of Raman scattering due to high concentration of electric near-field and large contact area between mitochondria and nanostructured surfaces.

U2 - 10.1038/srep13793

DO - 10.1038/srep13793

M3 - Journal article

C2 - 26346634

SN - 2045-2322

VL - 5

SP - 1

EP - 13

JO - Scientific Reports

JF - Scientific Reports

M1 - 13793

ER -

Probing cytochrome c in living mitochondria with surface-enhanced Raman spectroscopy

Abstract

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