Prevention of hyperglycemia in Zucker diabetic fatty rats by exercise training: effects on gene expression in insulin-sensitive tissues determined by high-density oligonucleotide microarray analysis.

Michele Colombo; Soeren Gregersen; Mogens Kruhoeffer; Andreas Agger; Jianzhong Xiao; Per Bendix Jeppesen; Torben Orntoft; Thorkil Ploug; Henrik Galbo; Kjeld Hermansen

doi:10.1016/j.metabol.2005.06.003

Prevention of hyperglycemia in Zucker diabetic fatty rats by exercise training: effects on gene expression in insulin-sensitive tissues determined by high-density oligonucleotide microarray analysis.

Michele Colombo, Soeren Gregersen, Mogens Kruhoeffer, Andreas Agger, Jianzhong Xiao, Per Bendix Jeppesen, Torben Orntoft, Thorkil Ploug, Henrik Galbo, Kjeld Hermansen

25 Citations (Scopus)

Abstract

Exercise training (ET) causes metabolic improvement in the prediabetic and diabetic states. However, only little information exists on the changes to ET at the transcriptional level in insulin-sensitive tissues. We have investigated the gene expression changes in skeletal muscle, liver, fat, and pancreatic islets after ET in male Zucker diabetic fatty (ZDF) rats. Eighteen ZDF rats (7 weeks old) were divided in a control and ET group. Exercise was performed using a motorized treadmill (20 m/min 1 hour daily for 6 days a week). Blood glucose, weight, and food intake were measured weekly. After 5 weeks, blood samples, soleus muscle, liver, visceral fat (epididymal fat pads), and islet tissue were collected. Gene expression was quantified with Affymetrix RG-U34A array (16 chips). Exercise training ameliorates the development of hyperglycemia and reduces plasma free fatty acid and the level of glucagon-insulin ratio (P < .05). In skeletal muscle, the expression of 302 genes increased, whereas that of 119 genes decreased. These changes involved genes related to skeletal muscle plasticity, Ca(2+) signals, energy metabolism (eg, glucose transporter 1, phosphorylase kinase), and other signaling pathways as well as genes with unknown functions (expressed sequence tags). In the liver, expression of 148 genes increased, whereas that of 199 genes decreased. These were primarily genes involved in lipogenesis and detoxification. Genes coding for transcription factors were changed in parallel in skeletal muscle and liver tissue. Training did not markedly influence the gene expression in islets. In conclusion, ET changes the expression of multiple genes in the soleus muscle and liver tissue and counteracts the development of diabetes, indicating that ET-induced changes in gene transcription may play an important role en the prevention of diabetes.

Original language	English
Journal	Metabolism - Clinical and Experimental
Volume	54
Issue number	12
Pages (from-to)	1571-81
Number of pages	10
ISSN	0026-0495
DOIs	https://doi.org/10.1016/j.metabol.2005.06.003
Publication status	Published - 2005

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Colombo, M., Gregersen, S., Kruhoeffer, M., Agger, A., Xiao, J., Jeppesen, P. B., Orntoft, T., Ploug, T., Galbo, H., & Hermansen, K. (2005). Prevention of hyperglycemia in Zucker diabetic fatty rats by exercise training: effects on gene expression in insulin-sensitive tissues determined by high-density oligonucleotide microarray analysis. Metabolism - Clinical and Experimental, 54(12), 1571-81. https://doi.org/10.1016/j.metabol.2005.06.003

Prevention of hyperglycemia in Zucker diabetic fatty rats by exercise training: effects on gene expression in insulin-sensitive tissues determined by high-density oligonucleotide microarray analysis. / Colombo, Michele; Gregersen, Soeren; Kruhoeffer, Mogens et al.
In: Metabolism - Clinical and Experimental, Vol. 54, No. 12, 2005, p. 1571-81.

Research output: Contribution to journal › Journal article › Research › peer-review

Colombo, M, Gregersen, S, Kruhoeffer, M, Agger, A, Xiao, J, Jeppesen, PB, Orntoft, T, Ploug, T , Galbo, H & Hermansen, K 2005, 'Prevention of hyperglycemia in Zucker diabetic fatty rats by exercise training: effects on gene expression in insulin-sensitive tissues determined by high-density oligonucleotide microarray analysis.', Metabolism - Clinical and Experimental, vol. 54, no. 12, pp. 1571-81. https://doi.org/10.1016/j.metabol.2005.06.003

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title = "Prevention of hyperglycemia in Zucker diabetic fatty rats by exercise training: effects on gene expression in insulin-sensitive tissues determined by high-density oligonucleotide microarray analysis.",

abstract = "Exercise training (ET) causes metabolic improvement in the prediabetic and diabetic states. However, only little information exists on the changes to ET at the transcriptional level in insulin-sensitive tissues. We have investigated the gene expression changes in skeletal muscle, liver, fat, and pancreatic islets after ET in male Zucker diabetic fatty (ZDF) rats. Eighteen ZDF rats (7 weeks old) were divided in a control and ET group. Exercise was performed using a motorized treadmill (20 m/min 1 hour daily for 6 days a week). Blood glucose, weight, and food intake were measured weekly. After 5 weeks, blood samples, soleus muscle, liver, visceral fat (epididymal fat pads), and islet tissue were collected. Gene expression was quantified with Affymetrix RG-U34A array (16 chips). Exercise training ameliorates the development of hyperglycemia and reduces plasma free fatty acid and the level of glucagon-insulin ratio (P < .05). In skeletal muscle, the expression of 302 genes increased, whereas that of 119 genes decreased. These changes involved genes related to skeletal muscle plasticity, Ca(2+) signals, energy metabolism (eg, glucose transporter 1, phosphorylase kinase), and other signaling pathways as well as genes with unknown functions (expressed sequence tags). In the liver, expression of 148 genes increased, whereas that of 199 genes decreased. These were primarily genes involved in lipogenesis and detoxification. Genes coding for transcription factors were changed in parallel in skeletal muscle and liver tissue. Training did not markedly influence the gene expression in islets. In conclusion, ET changes the expression of multiple genes in the soleus muscle and liver tissue and counteracts the development of diabetes, indicating that ET-induced changes in gene transcription may play an important role en the prevention of diabetes.",

author = "Michele Colombo and Soeren Gregersen and Mogens Kruhoeffer and Andreas Agger and Jianzhong Xiao and Jeppesen, {Per Bendix} and Torben Orntoft and Thorkil Ploug and Henrik Galbo and Kjeld Hermansen",

note = "Keywords: Adipose Tissue; Animals; Blood Glucose; Diabetes Mellitus, Type 2; Gene Expression Profiling; Hyperglycemia; Islets of Langerhans; Liver; Male; Muscle, Skeletal; Oligonucleotide Array Sequence Analysis; Physical Conditioning, Animal; RNA, Messenger; Rats; Rats, Zucker; Signal Transduction; Viscera",

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doi = "10.1016/j.metabol.2005.06.003",

language = "English",

volume = "54",

pages = "1571--81",

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T1 - Prevention of hyperglycemia in Zucker diabetic fatty rats by exercise training: effects on gene expression in insulin-sensitive tissues determined by high-density oligonucleotide microarray analysis.

AU - Colombo, Michele

AU - Gregersen, Soeren

AU - Kruhoeffer, Mogens

AU - Agger, Andreas

AU - Xiao, Jianzhong

AU - Jeppesen, Per Bendix

AU - Orntoft, Torben

AU - Ploug, Thorkil

AU - Galbo, Henrik

AU - Hermansen, Kjeld

N1 - Keywords: Adipose Tissue; Animals; Blood Glucose; Diabetes Mellitus, Type 2; Gene Expression Profiling; Hyperglycemia; Islets of Langerhans; Liver; Male; Muscle, Skeletal; Oligonucleotide Array Sequence Analysis; Physical Conditioning, Animal; RNA, Messenger; Rats; Rats, Zucker; Signal Transduction; Viscera

PY - 2005

Y1 - 2005

N2 - Exercise training (ET) causes metabolic improvement in the prediabetic and diabetic states. However, only little information exists on the changes to ET at the transcriptional level in insulin-sensitive tissues. We have investigated the gene expression changes in skeletal muscle, liver, fat, and pancreatic islets after ET in male Zucker diabetic fatty (ZDF) rats. Eighteen ZDF rats (7 weeks old) were divided in a control and ET group. Exercise was performed using a motorized treadmill (20 m/min 1 hour daily for 6 days a week). Blood glucose, weight, and food intake were measured weekly. After 5 weeks, blood samples, soleus muscle, liver, visceral fat (epididymal fat pads), and islet tissue were collected. Gene expression was quantified with Affymetrix RG-U34A array (16 chips). Exercise training ameliorates the development of hyperglycemia and reduces plasma free fatty acid and the level of glucagon-insulin ratio (P < .05). In skeletal muscle, the expression of 302 genes increased, whereas that of 119 genes decreased. These changes involved genes related to skeletal muscle plasticity, Ca(2+) signals, energy metabolism (eg, glucose transporter 1, phosphorylase kinase), and other signaling pathways as well as genes with unknown functions (expressed sequence tags). In the liver, expression of 148 genes increased, whereas that of 199 genes decreased. These were primarily genes involved in lipogenesis and detoxification. Genes coding for transcription factors were changed in parallel in skeletal muscle and liver tissue. Training did not markedly influence the gene expression in islets. In conclusion, ET changes the expression of multiple genes in the soleus muscle and liver tissue and counteracts the development of diabetes, indicating that ET-induced changes in gene transcription may play an important role en the prevention of diabetes.

AB - Exercise training (ET) causes metabolic improvement in the prediabetic and diabetic states. However, only little information exists on the changes to ET at the transcriptional level in insulin-sensitive tissues. We have investigated the gene expression changes in skeletal muscle, liver, fat, and pancreatic islets after ET in male Zucker diabetic fatty (ZDF) rats. Eighteen ZDF rats (7 weeks old) were divided in a control and ET group. Exercise was performed using a motorized treadmill (20 m/min 1 hour daily for 6 days a week). Blood glucose, weight, and food intake were measured weekly. After 5 weeks, blood samples, soleus muscle, liver, visceral fat (epididymal fat pads), and islet tissue were collected. Gene expression was quantified with Affymetrix RG-U34A array (16 chips). Exercise training ameliorates the development of hyperglycemia and reduces plasma free fatty acid and the level of glucagon-insulin ratio (P < .05). In skeletal muscle, the expression of 302 genes increased, whereas that of 119 genes decreased. These changes involved genes related to skeletal muscle plasticity, Ca(2+) signals, energy metabolism (eg, glucose transporter 1, phosphorylase kinase), and other signaling pathways as well as genes with unknown functions (expressed sequence tags). In the liver, expression of 148 genes increased, whereas that of 199 genes decreased. These were primarily genes involved in lipogenesis and detoxification. Genes coding for transcription factors were changed in parallel in skeletal muscle and liver tissue. Training did not markedly influence the gene expression in islets. In conclusion, ET changes the expression of multiple genes in the soleus muscle and liver tissue and counteracts the development of diabetes, indicating that ET-induced changes in gene transcription may play an important role en the prevention of diabetes.

U2 - 10.1016/j.metabol.2005.06.003

DO - 10.1016/j.metabol.2005.06.003

M3 - Journal article

C2 - 16311088

SN - 0026-0495

VL - 54

SP - 1571

EP - 1581

JO - Metabolism - Clinical and Experimental

JF - Metabolism - Clinical and Experimental

IS - 12

ER -

Prevention of hyperglycemia in Zucker diabetic fatty rats by exercise training: effects on gene expression in insulin-sensitive tissues determined by high-density oligonucleotide microarray analysis.

Abstract

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