Preservation of beta cell function in adult human pancreatic islets for several months in vitro

J Brunstedt; A Andersson; C Frimodt-Møller; Jens Høiriis Nielsen

Preservation of beta cell function in adult human pancreatic islets for several months in vitro

J Brunstedt, A Andersson, C Frimodt-Møller, Jens Høiriis Nielsen

Inflammation, Metabolism and Oxidation

34 Citations (Scopus)

Abstract

Islets of Langerhans were isolated from four human kidney donors, aged 16 to 21 years by the collagenase method described for isolation of rodent islets. So far the human islets have been kept in tissue culture, without attachment, in medium RPMI 1640 supplemented with 10% calf serum for more than 9 months, with preservation of the ability to release insulin in response to glucose stimulation. Replacement of calf serum with serum from normal human subjects did not affect B-cell survival, but resulted in elevated insulin values partly due to lower insulin degrading activity. Thus the described technique presents a valuable tool for studying chronic effects of metabolites and hormones on islet function, as well as for islet storage prior to transplantation into humans.

Original language	English
Journal	Diabetologia
Volume	16
Issue number	2
Pages (from-to)	97-100
Number of pages	4
ISSN	0012-186X
Publication status	Published - Feb 1979

Keywords

Adolescent
Adult
Culture Media
Culture Techniques
Glucose
Humans
Insulin
Islets of Langerhans
Tissue Preservation

Cite this

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title = "Preservation of beta cell function in adult human pancreatic islets for several months in vitro",

abstract = "Islets of Langerhans were isolated from four human kidney donors, aged 16 to 21 years by the collagenase method described for isolation of rodent islets. So far the human islets have been kept in tissue culture, without attachment, in medium RPMI 1640 supplemented with 10% calf serum for more than 9 months, with preservation of the ability to release insulin in response to glucose stimulation. Replacement of calf serum with serum from normal human subjects did not affect B-cell survival, but resulted in elevated insulin values partly due to lower insulin degrading activity. Thus the described technique presents a valuable tool for studying chronic effects of metabolites and hormones on islet function, as well as for islet storage prior to transplantation into humans.",

keywords = "Adolescent, Adult, Culture Media, Culture Techniques, Glucose, Humans, Insulin, Islets of Langerhans, Tissue Preservation",

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T1 - Preservation of beta cell function in adult human pancreatic islets for several months in vitro

AU - Brunstedt, J

AU - Andersson, A

AU - Frimodt-Møller, C

AU - Nielsen, Jens Høiriis

PY - 1979/2

Y1 - 1979/2

N2 - Islets of Langerhans were isolated from four human kidney donors, aged 16 to 21 years by the collagenase method described for isolation of rodent islets. So far the human islets have been kept in tissue culture, without attachment, in medium RPMI 1640 supplemented with 10% calf serum for more than 9 months, with preservation of the ability to release insulin in response to glucose stimulation. Replacement of calf serum with serum from normal human subjects did not affect B-cell survival, but resulted in elevated insulin values partly due to lower insulin degrading activity. Thus the described technique presents a valuable tool for studying chronic effects of metabolites and hormones on islet function, as well as for islet storage prior to transplantation into humans.

AB - Islets of Langerhans were isolated from four human kidney donors, aged 16 to 21 years by the collagenase method described for isolation of rodent islets. So far the human islets have been kept in tissue culture, without attachment, in medium RPMI 1640 supplemented with 10% calf serum for more than 9 months, with preservation of the ability to release insulin in response to glucose stimulation. Replacement of calf serum with serum from normal human subjects did not affect B-cell survival, but resulted in elevated insulin values partly due to lower insulin degrading activity. Thus the described technique presents a valuable tool for studying chronic effects of metabolites and hormones on islet function, as well as for islet storage prior to transplantation into humans.

KW - Adolescent

KW - Adult

KW - Culture Media

KW - Culture Techniques

KW - Glucose

KW - Humans

KW - Insulin

KW - Islets of Langerhans

KW - Tissue Preservation

M3 - Journal article

C2 - 365659

SN - 0012-186X

VL - 16

SP - 97

EP - 100

JO - Diabetologia

JF - Diabetologia

IS - 2

ER -

Preservation of beta cell function in adult human pancreatic islets for several months in vitro

Abstract

Keywords

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