Potential metabolic markers in glaucoma and their regulation in response to hypoxia

Rupali Vohra; Line Marie Dalgaard; Jeppe Vibaek; Mia Aggergaard Langbøl; Linda Hildegaard Bergersen; Niels Vidiendal Olsen; Bjørnar Hassel; Farrukh Abbas Chaudhry; Miriam Kolko

doi:10.1111/aos.14021

Potential metabolic markers in glaucoma and their regulation in response to hypoxia

Rupali Vohra, Line Marie Dalgaard, Jeppe Vibaek, Mia Aggergaard Langbøl, Linda Hildegaard Bergersen, Niels Vidiendal Olsen, Bjørnar Hassel, Farrukh Abbas Chaudhry, Miriam Kolko

9 Citations (Scopus)

Abstract

PURPOSE: To assess novel differences in serum levels of glucose, lactate and amino acids in patients with normal-tension glaucoma (NTG) compared to age-matched controls, at baseline and in response to universal hypoxia.

METHODS: Twelve patients diagnosed with NTG and eleven control subjects underwent normobaric hypoxia for 2 hr. Peripheral venous blood samples were taken at baseline, during hypoxia and in the recovery phase. Serum glucose and lactate levels were measured by a blood gas analyser. Amino acids were analysed by high-performance liquid chromatography.

RESULTS: Baseline levels of lactate and total amino acids were significantly lower in patients with NTG compared to healthy controls. No differences were seen in blood glucose levels between the two groups. Lactate levels remained unchanged during hypoxia in the control group, but increased in patients with NTG. In the recovery phase, total amino acid levels were reduced in the control group, whereas no changes were found in patients with NTG.

CONCLUSION: Reduced serum levels of lactate and total amino acids were identified as potential markers for NTG. Moreover, significant differential regulatory patterns of certain amino acids were found in patients with NTG compared to control subjects. Overall, our results suggest a link between systemic energy metabolites and NTG and support a novel understanding of glaucoma as an inner retinal manifestation of a systemic condition.

Original language	English
Journal	Acta Ophthalmologica
Volume	97
Issue number	6
Pages (from-to)	567-576
ISSN	1755-375X
DOIs	https://doi.org/10.1111/aos.14021
Publication status	Published - 28 Jan 2019

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@article{a942738a6cbb45c0bd349fcc0353e687,

title = "Potential metabolic markers in glaucoma and their regulation in response to hypoxia",

abstract = "PURPOSE: To assess novel differences in serum levels of glucose, lactate and amino acids in patients with normal-tension glaucoma (NTG) compared to age-matched controls, at baseline and in response to universal hypoxia.METHODS: Twelve patients diagnosed with NTG and eleven control subjects underwent normobaric hypoxia for 2 hr. Peripheral venous blood samples were taken at baseline, during hypoxia and in the recovery phase. Serum glucose and lactate levels were measured by a blood gas analyser. Amino acids were analysed by high-performance liquid chromatography.RESULTS: Baseline levels of lactate and total amino acids were significantly lower in patients with NTG compared to healthy controls. No differences were seen in blood glucose levels between the two groups. Lactate levels remained unchanged during hypoxia in the control group, but increased in patients with NTG. In the recovery phase, total amino acid levels were reduced in the control group, whereas no changes were found in patients with NTG.CONCLUSION: Reduced serum levels of lactate and total amino acids were identified as potential markers for NTG. Moreover, significant differential regulatory patterns of certain amino acids were found in patients with NTG compared to control subjects. Overall, our results suggest a link between systemic energy metabolites and NTG and support a novel understanding of glaucoma as an inner retinal manifestation of a systemic condition.",

author = "Rupali Vohra and Dalgaard, {Line Marie} and Jeppe Vibaek and Langb{\o}l, {Mia Aggergaard} and Bergersen, {Linda Hildegaard} and Olsen, {Niels Vidiendal} and Bj{\o}rnar Hassel and Chaudhry, {Farrukh Abbas} and Miriam Kolko",

note = "{\textcopyright} 2019 Acta Ophthalmologica Scandinavica Foundation. Published by John Wiley & Sons Ltd.",

year = "2019",

month = jan,

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doi = "10.1111/aos.14021",

language = "English",

volume = "97",

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TY - JOUR

T1 - Potential metabolic markers in glaucoma and their regulation in response to hypoxia

AU - Vohra, Rupali

AU - Dalgaard, Line Marie

AU - Vibaek, Jeppe

AU - Langbøl, Mia Aggergaard

AU - Bergersen, Linda Hildegaard

AU - Olsen, Niels Vidiendal

AU - Hassel, Bjørnar

AU - Chaudhry, Farrukh Abbas

AU - Kolko, Miriam

PY - 2019/1/28

Y1 - 2019/1/28

N2 - PURPOSE: To assess novel differences in serum levels of glucose, lactate and amino acids in patients with normal-tension glaucoma (NTG) compared to age-matched controls, at baseline and in response to universal hypoxia.METHODS: Twelve patients diagnosed with NTG and eleven control subjects underwent normobaric hypoxia for 2 hr. Peripheral venous blood samples were taken at baseline, during hypoxia and in the recovery phase. Serum glucose and lactate levels were measured by a blood gas analyser. Amino acids were analysed by high-performance liquid chromatography.RESULTS: Baseline levels of lactate and total amino acids were significantly lower in patients with NTG compared to healthy controls. No differences were seen in blood glucose levels between the two groups. Lactate levels remained unchanged during hypoxia in the control group, but increased in patients with NTG. In the recovery phase, total amino acid levels were reduced in the control group, whereas no changes were found in patients with NTG.CONCLUSION: Reduced serum levels of lactate and total amino acids were identified as potential markers for NTG. Moreover, significant differential regulatory patterns of certain amino acids were found in patients with NTG compared to control subjects. Overall, our results suggest a link between systemic energy metabolites and NTG and support a novel understanding of glaucoma as an inner retinal manifestation of a systemic condition.

AB - PURPOSE: To assess novel differences in serum levels of glucose, lactate and amino acids in patients with normal-tension glaucoma (NTG) compared to age-matched controls, at baseline and in response to universal hypoxia.METHODS: Twelve patients diagnosed with NTG and eleven control subjects underwent normobaric hypoxia for 2 hr. Peripheral venous blood samples were taken at baseline, during hypoxia and in the recovery phase. Serum glucose and lactate levels were measured by a blood gas analyser. Amino acids were analysed by high-performance liquid chromatography.RESULTS: Baseline levels of lactate and total amino acids were significantly lower in patients with NTG compared to healthy controls. No differences were seen in blood glucose levels between the two groups. Lactate levels remained unchanged during hypoxia in the control group, but increased in patients with NTG. In the recovery phase, total amino acid levels were reduced in the control group, whereas no changes were found in patients with NTG.CONCLUSION: Reduced serum levels of lactate and total amino acids were identified as potential markers for NTG. Moreover, significant differential regulatory patterns of certain amino acids were found in patients with NTG compared to control subjects. Overall, our results suggest a link between systemic energy metabolites and NTG and support a novel understanding of glaucoma as an inner retinal manifestation of a systemic condition.

U2 - 10.1111/aos.14021

DO - 10.1111/aos.14021

M3 - Journal article

C2 - 30690927

SN - 1755-375X

VL - 97

SP - 567

EP - 576

JO - Acta Ophthalmologica

JF - Acta Ophthalmologica

IS - 6

ER -

Potential metabolic markers in glaucoma and their regulation in response to hypoxia

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