Potassium and calcium channel gene expression in small arteries in porcine and rat models of diet-induced obesity (Poster)

Lars Jørn Jensen; Max Salomonsson; Charlotte Mehlin Sørensen; Niels-Henrik von Holstein-Rathlou; Henrik Duelund Pedersen; Berit Ø. Christoffersen; Lisbeth Høier Olsen; Trine Pagh Ludvigsen

doi:10.1096/fj.1530-6860

Potassium and calcium channel gene expression in small arteries in porcine and rat models of diet-induced obesity (Poster)

Lars Jørn Jensen, Max Salomonsson, Charlotte Mehlin Sørensen, Niels-Henrik von Holstein-Rathlou, Henrik Duelund Pedersen, Berit Ø. Christoffersen, Lisbeth Høier Olsen, Trine Pagh Ludvigsen

Abstract

Obesity is an increasing problem worldwide leading to cardiovascular morbidity. Only limited information exists on the transcriptional regulation of arterial K+ and Ca2+ channels in obesity. We quantified, by real-time PCR, mRNA expression of K+ channels and L-type Ca2+ channels (LTCC) in small mesenteric (MA), middle cerebral (MCA), and left coronary arteries (LCA) of lean vs. obese rats and minipigs. Male Sprague Dawley rats were fed a high-fat (FAT; N=5), high-fructose (FRUC; N=7), high-fat/high-fructose (FAT/FRUC; N=7) or standard diet (STD; N=7-11) for 28 Weeks. FAT and FAT/FRUC became obese, whereas FRUC and STD were lean. Systolic blood pressure (SBP) averaged over 14 weeks was increased (P<0.001) in FRUC and FAT/FRUC but not in FAT. Kir, Kv1.2, Kv1.5, and Kv7.4 channels were up-regulated in FAT, whereas SKca and IKca channels were down-regulated in FAT/FRUC (P<0.05). There were no transcriptional changes in FRUC. Castrated male Göttingen minipigs with or without diabetes were fed a diet rich in fat, cholesterol and fructose (OB+DIAB; N=3 vs. OB; N=2, respectively) or a standard diet (STD; N=3) for 22-45 weeks. Body weight, total body fat content and plasma triglyceride levels were increased in OB and OB+DIAB. BKca, IKca, SKca and/or LTCC mRNA was up-regulated in LCA from OB and OB+DIAB (n.s.). Expression of BKca mRNA was increased, whereas IKca mRNA decreased in MCA from OB (n.s.). SKca mRNA was decreased in MA from OB (n.s.). Diet-induced obesity in rats and minipigs lead to complex changes in K+ and Ca2+ channel gene expression, which, in rats, did not seem to be linked with changes in SBP. These transcriptional changes may lead to disturbances in microvascular flow patterns in obesity.

Original language	English
Article number	676.4
Journal	FASEB journal : official publication of the Federation of American Societies for Experimental Biology
Volume	28
Issue number	Supplement 1
Number of pages	1
ISSN	0892-6638
DOIs	https://doi.org/10.1096/fj.1530-6860
Publication status	Published - 27 Apr 2014
Event	Experimental Biology - San Diego, United States Duration: 26 Apr 2014 → 30 Apr 2014

Conference

Conference	Experimental Biology
Country/Territory	United States
City	San Diego
Period	26/04/2014 → 30/04/2014

Keywords

Faculty of Health and Medical Sciences
Obesity
Arteries
Potassium Channels
Calcium Channels
Gene expression
metabolic syndrome
Rat
Pig

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1096/fj.1530-6860

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Jensen, L. J., Salomonsson, M., Sørensen, C. M., von Holstein-Rathlou, N-H., Pedersen, H. D., Christoffersen, B. Ø., Olsen, L. H., & Ludvigsen, T. P. (2014). Potassium and calcium channel gene expression in small arteries in porcine and rat models of diet-induced obesity (Poster). FASEB journal : official publication of the Federation of American Societies for Experimental Biology, 28(Supplement 1), [676.4]. https://doi.org/10.1096/fj.1530-6860

Potassium and calcium channel gene expression in small arteries in porcine and rat models of diet-induced obesity (Poster). / Jensen, Lars Jørn ; Salomonsson, Max ; Sørensen, Charlotte Mehlin et al.

In: FASEB journal : official publication of the Federation of American Societies for Experimental Biology, Vol. 28, No. Supplement 1, 676.4, 27.04.2014.

Research output: Contribution to journal › Conference abstract in journal › Research › peer-review

Jensen, LJ , Salomonsson, M , Sørensen, CM , von Holstein-Rathlou, N-H, Pedersen, HD, Christoffersen, BØ, Olsen, LH & Ludvigsen, TP 2014, 'Potassium and calcium channel gene expression in small arteries in porcine and rat models of diet-induced obesity (Poster)', FASEB journal : official publication of the Federation of American Societies for Experimental Biology, vol. 28, no. Supplement 1, 676.4. https://doi.org/10.1096/fj.1530-6860

Jensen LJ , Salomonsson M , Sørensen CM , von Holstein-Rathlou N-H, Pedersen HD, Christoffersen BØ et al. Potassium and calcium channel gene expression in small arteries in porcine and rat models of diet-induced obesity (Poster). FASEB journal : official publication of the Federation of American Societies for Experimental Biology. 2014 Apr 27;28(Supplement 1):676.4. doi: 10.1096/fj.1530-6860

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abstract = "Obesity is an increasing problem worldwide leading to cardiovascular morbidity. Only limited information exists on the transcriptional regulation of arterial K+ and Ca2+ channels in obesity. We quantified, by real-time PCR, mRNA expression of K+ channels and L-type Ca2+ channels (LTCC) in small mesenteric (MA), middle cerebral (MCA), and left coronary arteries (LCA) of lean vs. obese rats and minipigs. Male Sprague Dawley rats were fed a high-fat (FAT; N=5), high-fructose (FRUC; N=7), high-fat/high-fructose (FAT/FRUC; N=7) or standard diet (STD; N=7-11) for 28 Weeks. FAT and FAT/FRUC became obese, whereas FRUC and STD were lean. Systolic blood pressure (SBP) averaged over 14 weeks was increased (P<0.001) in FRUC and FAT/FRUC but not in FAT. Kir, Kv1.2, Kv1.5, and Kv7.4 channels were up-regulated in FAT, whereas SKca and IKca channels were down-regulated in FAT/FRUC (P<0.05). There were no transcriptional changes in FRUC. Castrated male G{\"o}ttingen minipigs with or without diabetes were fed a diet rich in fat, cholesterol and fructose (OB+DIAB; N=3 vs. OB; N=2, respectively) or a standard diet (STD; N=3) for 22-45 weeks. Body weight, total body fat content and plasma triglyceride levels were increased in OB and OB+DIAB. BKca, IKca, SKca and/or LTCC mRNA was up-regulated in LCA from OB and OB+DIAB (n.s.). Expression of BKca mRNA was increased, whereas IKca mRNA decreased in MCA from OB (n.s.). SKca mRNA was decreased in MA from OB (n.s.). Diet-induced obesity in rats and minipigs lead to complex changes in K+ and Ca2+ channel gene expression, which, in rats, did not seem to be linked with changes in SBP. These transcriptional changes may lead to disturbances in microvascular flow patterns in obesity.",

keywords = "Faculty of Health and Medical Sciences, Obesity, Arteries, Potassium Channels, Calcium Channels, Gene expression, metabolic syndrome, Rat, Pig",

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T1 - Potassium and calcium channel gene expression in small arteries in porcine and rat models of diet-induced obesity (Poster)

AU - Jensen, Lars Jørn

AU - Salomonsson, Max

AU - Sørensen, Charlotte Mehlin

AU - von Holstein-Rathlou, Niels-Henrik

AU - Pedersen, Henrik Duelund

AU - Christoffersen, Berit Ø.

AU - Olsen, Lisbeth Høier

AU - Ludvigsen, Trine Pagh

N1 - Faseb Journal, Vol. 28, No. 1 Suppl. 676.4

PY - 2014/4/27

Y1 - 2014/4/27

N2 - Obesity is an increasing problem worldwide leading to cardiovascular morbidity. Only limited information exists on the transcriptional regulation of arterial K+ and Ca2+ channels in obesity. We quantified, by real-time PCR, mRNA expression of K+ channels and L-type Ca2+ channels (LTCC) in small mesenteric (MA), middle cerebral (MCA), and left coronary arteries (LCA) of lean vs. obese rats and minipigs. Male Sprague Dawley rats were fed a high-fat (FAT; N=5), high-fructose (FRUC; N=7), high-fat/high-fructose (FAT/FRUC; N=7) or standard diet (STD; N=7-11) for 28 Weeks. FAT and FAT/FRUC became obese, whereas FRUC and STD were lean. Systolic blood pressure (SBP) averaged over 14 weeks was increased (P<0.001) in FRUC and FAT/FRUC but not in FAT. Kir, Kv1.2, Kv1.5, and Kv7.4 channels were up-regulated in FAT, whereas SKca and IKca channels were down-regulated in FAT/FRUC (P<0.05). There were no transcriptional changes in FRUC. Castrated male Göttingen minipigs with or without diabetes were fed a diet rich in fat, cholesterol and fructose (OB+DIAB; N=3 vs. OB; N=2, respectively) or a standard diet (STD; N=3) for 22-45 weeks. Body weight, total body fat content and plasma triglyceride levels were increased in OB and OB+DIAB. BKca, IKca, SKca and/or LTCC mRNA was up-regulated in LCA from OB and OB+DIAB (n.s.). Expression of BKca mRNA was increased, whereas IKca mRNA decreased in MCA from OB (n.s.). SKca mRNA was decreased in MA from OB (n.s.). Diet-induced obesity in rats and minipigs lead to complex changes in K+ and Ca2+ channel gene expression, which, in rats, did not seem to be linked with changes in SBP. These transcriptional changes may lead to disturbances in microvascular flow patterns in obesity.

AB - Obesity is an increasing problem worldwide leading to cardiovascular morbidity. Only limited information exists on the transcriptional regulation of arterial K+ and Ca2+ channels in obesity. We quantified, by real-time PCR, mRNA expression of K+ channels and L-type Ca2+ channels (LTCC) in small mesenteric (MA), middle cerebral (MCA), and left coronary arteries (LCA) of lean vs. obese rats and minipigs. Male Sprague Dawley rats were fed a high-fat (FAT; N=5), high-fructose (FRUC; N=7), high-fat/high-fructose (FAT/FRUC; N=7) or standard diet (STD; N=7-11) for 28 Weeks. FAT and FAT/FRUC became obese, whereas FRUC and STD were lean. Systolic blood pressure (SBP) averaged over 14 weeks was increased (P<0.001) in FRUC and FAT/FRUC but not in FAT. Kir, Kv1.2, Kv1.5, and Kv7.4 channels were up-regulated in FAT, whereas SKca and IKca channels were down-regulated in FAT/FRUC (P<0.05). There were no transcriptional changes in FRUC. Castrated male Göttingen minipigs with or without diabetes were fed a diet rich in fat, cholesterol and fructose (OB+DIAB; N=3 vs. OB; N=2, respectively) or a standard diet (STD; N=3) for 22-45 weeks. Body weight, total body fat content and plasma triglyceride levels were increased in OB and OB+DIAB. BKca, IKca, SKca and/or LTCC mRNA was up-regulated in LCA from OB and OB+DIAB (n.s.). Expression of BKca mRNA was increased, whereas IKca mRNA decreased in MCA from OB (n.s.). SKca mRNA was decreased in MA from OB (n.s.). Diet-induced obesity in rats and minipigs lead to complex changes in K+ and Ca2+ channel gene expression, which, in rats, did not seem to be linked with changes in SBP. These transcriptional changes may lead to disturbances in microvascular flow patterns in obesity.

KW - Faculty of Health and Medical Sciences

KW - Obesity

KW - Arteries

KW - Potassium Channels

KW - Calcium Channels

KW - Gene expression

KW - metabolic syndrome

KW - Rat

KW - Pig

U2 - 10.1096/fj.1530-6860

DO - 10.1096/fj.1530-6860

M3 - Conference abstract in journal

SN - 0892-6638

VL - 28

JO - F A S E B Journal

JF - F A S E B Journal

IS - Supplement 1

M1 - 676.4

T2 - Experimental Biology

Y2 - 26 April 2014 through 30 April 2014

ER -

Potassium and calcium channel gene expression in small arteries in porcine and rat models of diet-induced obesity (Poster)

Abstract

Conference

Keywords

UN SDGs

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