Post mortem survival of gallibacterium anatis in a laying hen experimental infection model

TY - JOUR

T1 - Post mortem survival of gallibacterium anatis in a laying hen experimental infection model

AU - Wang, Chong

AU - Pors, Susanne Elisabeth

AU - Bojesen, Anders Miki

PY - 2018

Y1 - 2018

N2 - To assess the survival of Gallibacterium anatis in dead laying hens, 21-wk-old laying hens were injected intraperitoneally with 0.5 ml brain hearth infusion broth containing 108 colony-forming units (CFU) of G. anatis 12656-12 liver (n = 16), Escherichia coli ST141 (n = 16), or a mix of G. anatis 12656-12 liver and E. coli ST141 (n = 16), respectively. Birds were euthanatized 24 hr post injection. From each group eight dead birds were kept at 4 C and eight at room temperature. Swab samples were taken at different time points post euthanatization and streaked on blood agar plates. From the birds kept at 4 C, G. anatis was reisolated from the G. anatis and the G. anatis-E. coli co-injected groups at least 12 days post euthanization. From birds kept at room temperature, G. anatis was reisolated up to 2 days post euthanatization. When using the gyrB-based G. anatis-specific quantitative PCR (qPCR), G. anatis was detected within at least 5 days, and up to 5 days post euthanatization, from birds kept at room temperature and 4 C, respectively. Escherichia coli was reisolated from all the time points independent of how the birds were kept. No difference was observed between the reisolation rates for G. anatis or E. coli when comparing similar detection methods. For birds kept at 4 C, bacterial cultivation was a more sensitive method for detecting G. anatis (P < 0.05), whereas for birds kept at room temperature, the G. anatis-specific qPCR outperformed bacterial culture (P < 0.05). In conclusion, we demonstrated that G. anatis has a poorer survival rate than does E. coli in dead chickens kept at room temperature. That finding may affect the overall diagnostic sensitivity and lead to underdiagnosis of G. anatis in a normal production setting.

AB - To assess the survival of Gallibacterium anatis in dead laying hens, 21-wk-old laying hens were injected intraperitoneally with 0.5 ml brain hearth infusion broth containing 108 colony-forming units (CFU) of G. anatis 12656-12 liver (n = 16), Escherichia coli ST141 (n = 16), or a mix of G. anatis 12656-12 liver and E. coli ST141 (n = 16), respectively. Birds were euthanatized 24 hr post injection. From each group eight dead birds were kept at 4 C and eight at room temperature. Swab samples were taken at different time points post euthanatization and streaked on blood agar plates. From the birds kept at 4 C, G. anatis was reisolated from the G. anatis and the G. anatis-E. coli co-injected groups at least 12 days post euthanization. From birds kept at room temperature, G. anatis was reisolated up to 2 days post euthanatization. When using the gyrB-based G. anatis-specific quantitative PCR (qPCR), G. anatis was detected within at least 5 days, and up to 5 days post euthanatization, from birds kept at room temperature and 4 C, respectively. Escherichia coli was reisolated from all the time points independent of how the birds were kept. No difference was observed between the reisolation rates for G. anatis or E. coli when comparing similar detection methods. For birds kept at 4 C, bacterial cultivation was a more sensitive method for detecting G. anatis (P < 0.05), whereas for birds kept at room temperature, the G. anatis-specific qPCR outperformed bacterial culture (P < 0.05). In conclusion, we demonstrated that G. anatis has a poorer survival rate than does E. coli in dead chickens kept at room temperature. That finding may affect the overall diagnostic sensitivity and lead to underdiagnosis of G. anatis in a normal production setting.

KW - Escherichia coli

KW - Gallibacterium anatis

KW - laying hen infection model

KW - survival

U2 - 10.1637/11809-020818-Reg.1

DO - 10.1637/11809-020818-Reg.1

M3 - Journal article

C2 - 29613813

AN - SCOPUS:85049154686

SN - 0005-2086

VL - 62

SP - 195

EP - 200

JO - Avian Diseases

JF - Avian Diseases

IS - 2

ER -